Oceanobacillus polygoni sp. nov., a facultatively alkaliphile isolated from indigo fermentation fluid

Abstract

A facultatively alkaliphilic, lactic-acid-producing and halophilic strain, designated SA9T , was isolated from a fermented Polygonum indigo (Polygonum tinctorium Lour.) liquor sample prepared in a laboratory. The 16S rRNA gene sequence phylogeny suggested that strain SA9 T was a member of the genus Oceanobacillus with the closest relative being Oceanobacillus profundus KCCM 42318 T (99.3 % 16S rRNA gene sequence similarity). Cells of strain SA9 T stained Grampositive and were facultative anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 5 and 48 6C (optimum, 35 6C) and at pH 7-12 (optimum, pH 9). The isoprenoid quinone detected was menaquinone-7 (MK-7) and the DNA G+C content was 40.6±0.9 mol%. The whole-cell fatty acid profile mainly consisted of iso-C 15 : 0 , anteiso-C 15 : 0 , C 16 : 0 and anteiso-C 17 : 0 . DNA-DNA hybridization with Oceanobacillus profundus DSM 18246 T revealed a DNA-DNA relatedness value of 23±2 %. On the basis of the differences in phenotypic and chemotaxonomic characteristics, and the results of phylogenetic analyses based on 16S rRNA gene sequences and DNA-DNA relatedness data from recognized species of the genus Oceanobacillus, strain SA9 T merits classification as a representative of a novel species of the genus Oceanobacillus, for which the name Oceanobacillus polygoni sp. nov. is proposed. The type strain is SA9 T (5JCM 17252 T 5NCIMB 14684 T

Phylogenetic Profiling and Diversity of Bacterial Communities in the Death Valley, an Extreme Habitat in the Atacama Desert

“…At the time of writing, the genus Oceanobacillus comprises 13 species. Members of the genus Oceanobacillus are distributed in a wide range of environments including species isolated from deep-sea sediment, mural paintings, freshwater fish, algae, an insect, activated sludge bottom of a mould fermenter food, saline saltern and lakes, dune sand and fermentation liquor [29]. All Oceanobacillus clone sequences described in this study were related to halotolerant strains, a consistent result considering the concentration of salt detected in the site sampled.…”

Oceanobacillus halophilus sp. nov., a novel moderately halophilic bacterium from a hypersaline lake

“…The polar lipid pattern is also in line with those of other species of the genus Oceanobacillus in that phosphatidylglycerol and diphosphatidylglycerol are the two dominant compounds. However, the novel isolate contained some amino(glyco)lipids as the minor phospholipids, which were not detected in other phylogeneticaly related species (Kim et al, 2007;Namwong et al, 2009;Hirota et al, 2013).…”

Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

“…The genus Oceanobacillus was established by Lu et al (2001) and the description of the genus was emended by Yumoto et al (2005), Lee et al (2006) and Hirota et al (2013) on the basis of chemotaxonomic and phenotypic data. At the time of writing, this genus comprises 21 validly named species, including six recently described species, Oceanobacillus limi (Amoozegar et al 2014), Oceanobacillus luteolus (Wu et al 2014), Oceanobacillus arenosus (Kim et al 2015), Oceanobacillus bengalensis (Yongchang et al 2015), Oceanobacillus rekensis and Oceanobacillus damuensis (Long et al 2015).…”