Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms

McLaughlin, John; Padfield, Philip J.; Burt, Julian P.H.; O’Neill, CA

doi:10.1152/ajpcell.00007.2004

Cited by 124 publications

(78 citation statements)

References 27 publications

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“…Cldn3 function in the vertebrate intestine has yet to be clearly defined. Recent studies have reported that treatment of Caco-2 epithelia with a mycotoxin (ochratoxin A), caused a marked decrease (ϳ87%) in Cldn3 expression, which was coupled to a substantial reduction in TER and concomitant increase in paracellular permeability (29). These observations are in general accord with studies relating pathological dysfunction in the gastrointestinal tract to increased intestinal permeability, a phenomenon normally associated with reduced TJ protein expression and changes in TJ protein distribution (7).…”

Section: Discussionsupporting

confidence: 62%

Claudin-3 tight junction proteins inTetraodon nigroviridis: cloning, tissue-specific expression, and a role in hydromineral balance

Bagherie-Lachidan¹,

Wright²,

Kelly³

2008

American Journal of Physiology-Regulatory, Integrative and Comp

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Section: Discussionsupporting

confidence: 62%

Claudin-3 tight junction proteins inTetraodon nigroviridis: cloning, tissue-specific expression, and a role in hydromineral balance

Bagherie-Lachidan¹,

Wright²,

Kelly³

2008

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Protein was extracted from NHEK cells according to the method described by McLaughlin et al (19) ]) containing aprotinin (10 g/ml), leupeptin (10 g/ml), and pepstatin A (10 g/ml) and then incubated on ice for 30 min. Following centrifugation in a microcentrifuge, the supernatant was recovered in a clean Eppendorf tube and used for analysis of TJ protein expression.…”

Section: Methodsmentioning

confidence: 99%

“…The membranes were subsequently washed and incubated with horseradish peroxidase-conjugated secondary antibodies. The immunoblots were developed using enhanced chemiluminescence (Amersham, Bucks, United Kingdom), and densitometry was performed as described by McLaughlin et al (19).…”

Section: Methodsmentioning

confidence: 99%

Strain-Dependent Augmentation of Tight-Junction Barrier Function in Human Primary Epidermal Keratinocytes by Lactobacillus and Bifidobacterium Lysates

Sultana

McBain

O’Neill

2013

Appl Environ Microbiol

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128

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In this study, we investigated whether probiotic lysates can modify the tight-junction function of human primary keratinocytes. The keratinocytes were grown on cell culture inserts and treated with lysates from Bifidobacterium longum, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus fermentum, or Lactobacillus rhamnosus GG. With the exception of L. fermentum (which decreased cell viability), all strains markedly enhanced tight-junction barrier function within 24 h, as assessed by measurements of transepithelial electrical resistance (TEER). However, B. longum and L. rhamnosus GG were the most efficacious, producing dose-dependent increases in resistance that were maintained for 4 days. These increases in TEER correlated with elevated expression of tight-junction protein components. Neutralization of Toll-like receptor 2 abolished both the increase in TEER and expression of tight-junction proteins induced by B. longum, but not L. rhamnosus GG. These data suggest that some bacterial strains increase tight-junction function via modulation of protein components but the different pathways involved may vary depending on the bacterial strain.

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“…Sallee and Burridge (2009) reported that occludin and ZO-1 were dephosphorylated by DEP-1 in MCF10A human mammary epithelial cells treated with ochratoxin A. An increase of permeability was caused by the removal of claudin-3 and 4 by ochratoxin A treatment in Caco-2 cells (McLaughlin et al 2004). As such, we tested the effect of patulin and depletion of DEP-1 on the phosphorylation status of claudin-4.…”

Section: Effects Of Patulin or Knockdown Of Dep-1 On The Epithelial Bmentioning

confidence: 99%

The Mycotoxin Patulin Decreases Expression of Density-Enhanced Phosphatase-1 by Down-Regulating PPAR<i>γ</i> in Human Colon Cancer Cells

Katsuyama

Konno

Shimoyama

et al. 2014

Tohoku J. Exp. Med.

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Patulin is a mycotoxin that is found mainly in apple products and causes symptoms such as bleeding from the digestive tract and diarrhea. Efforts to elucidate the mechanism of its toxicity have focused on protein tyrosine phosphatases (PTPs), which regulate the function of tight junctions (TJs) in colon epithelial cells. Patulin reacts with the conserved cysteine residues in the catalytic domains of PTP isoforms. Treatment of Caco-2 human colon cancer cells, used as a colon epithelial model, with 50 µM patulin decreased the level of density-enhanced phosphatase-1 (DEP-1) protein to 30% of the control level after 6 h. The level of DEP-1 mRNA was also decreased during 24 h after treatment with patulin. Moreover, knockdown of DEP-1 increased the level of phosphorylated claudin-4. Destruction of TJs by patulin treatment was observed by immunostaining with an antibody against zonula occludens (ZO)-1. To better understand the mechanistic basis of the decrease in DEP-1 mRNA levels, we searched for a cis-element upstream of the DEP-1 gene and found an element responsive to the peroxisome proliferator-activated receptor gamma (PPARγ ) protein. Using a PPARγ-specific antibody, we showed a decrease in PPARγ abundance to 42% of the control level within 6 h after treatment with patulin. PPARγ has four cysteine residues that are involved in zinc finger formation. Our data suggest that DEP-1 affects TJ function and that PPARγ might control DEP-1 expression. Therefore, the toxicity of patulin to cellular functions might be attributable to its ability to down-regulate the expression of DEP-1 and PPARγ.

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Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms

Abstract: . Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms.

Cited by 124 publications

References 27 publications

Claudin-3 tight junction proteins inTetraodon nigroviridis: cloning, tissue-specific expression, and a role in hydromineral balance

Claudin-3 tight junction proteins inTetraodon nigroviridis: cloning, tissue-specific expression, and a role in hydromineral balance

Strain-Dependent Augmentation of Tight-Junction Barrier Function in Human Primary Epidermal Keratinocytes by Lactobacillus and Bifidobacterium Lysates

The Mycotoxin Patulin Decreases Expression of Density-Enhanced Phosphatase-1 by Down-Regulating PPAR<i>γ</i> in Human Colon Cancer Cells

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