Octupolar Plasmonic Nanosensor Based on Ordered Arrays of Triangular Au Nanopillars for Selective Rotavirus Detection

Rippa, Massimo; Castagna, Riccardo; Brandi, Sérgio Duarte; Fusco, Giovanna; Monini, Marina; Chen, Dong; Zhou, Jun; Zyss, Joseph; Petti, Lucia

doi:10.1021/acsanm.0c00872

Cited by 35 publications

(32 citation statements)

References 60 publications

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“…Nanostructured Au substrates were fabricated by following a procedure identical to the one already described. 19 Briefly, 300 μm × 300 μm Au nanostructures were fabricated by a high-resolution electron beam lithography (EBL) system (Raith 150 EBL system), using a 78 nm layer of styrene methyl acrylate (ZEP 520A) as positive resist. The resist was spin-coated on 2 × 2 cm glass slides covered with a 15 nm indium tin oxide (ITO) conductive layer and baked at 170 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

et al. 2022

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A long-term reusable sensor that provides the opportunity to easily regenerate the active surface and minimize the occurrence of undesired absorption events is an appealing solution that helps to cut down the costs and improve the device performances. Impressive advances have been made in the past years concerning the development of novel cutting-edge sensors, but the reusability can currently represent a challenge. Direct shielding of the sensor surface is not always applicable, because it can impact the device performance. This study reports an antiadhesive layer (AAL) made of 90 mg/mL DNA sodium salt from salmon testes (ssstDNA) for passivating gold plasmonic sensor surfaces. Our gold two-dimensional (2D) nanostructured plasmonic metasurfaces modified with AAL were used for DNA quantification. AAL is thin enough that the plasmonic sensor remains sensitive to subsequent deposition of DNA, which serves as an analyte. AAL protects the gold surface from unwanted nonspecific adsorption by enabling wash-off of the deposited analyte after analysis and thus recovery of the LSPR peak position (rLSPR). The calibration curve obtained on a single nanostructure (Achiral Octupolar, 100 nm pitch) gave an LOD = 105 ng/mL and an extraordinary dynamic range, performances comparable or superior to those of commercial UV–vis spectrometers for acid nucleic dosage. Two different analytes were tested: ssstDNA (∼2000 bp) in deionized water and double-strand DNA (dsDNA) of 546–1614 bp in 100 mM Tris buffer and 10 mM MgCl 2 . The two nanostructures (Achiral Octupolar 25 and 100) were found to have the same sensitivity to DNA in deionized water but different sensitivity to DNA in a salt/buffer solution, opening a potential for solute discrimination. To the best of our knowledge, this is the first report on the use of AAL made of several kilobase-pairs-long dsDNA to produce a reusable plasmonic sensor. The working principle and limitations are drawn based on the LSPR and SERS study.

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Section: Methodsmentioning

confidence: 99%

DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

et al. 2022

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“…Hence, different kinds of optical biosensors such as surface plasmon resonance (SPR), optical fibers, adsorption, fluorescent, and luminescence are currently available in the literature and on the market [ 98 ]. Nevertheless, the SPR strategy is the most known and used version of optical biosensors for the diagnosis of gastroenteritis viruses in publications [ 99 , 100 , 101 ].…”

Section: Investigation Of Various Biosensing Platforms For Detection ...mentioning

confidence: 99%

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

et al. 2022

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Gastroenteritis, as one of the main worldwide health challenges, especially in children, leads to 3–6 million deaths annually and causes nearly 20% of the total deaths of children aged ˂5 years, of which ~1.5 million gastroenteritis deaths occur in developing nations. Viruses are the main causative agent (~70%) of gastroenteritis episodes and their specific and early diagnosis via laboratory assays is very helpful for having successful antiviral therapy and reduction in infection burden. Regarding this importance, the present literature is the first review of updated improvements in the employing of different types of biosensors such as electrochemical, optical, and piezoelectric for sensitive, simple, cheap, rapid, and specific diagnosis of human gastroenteritis viruses. The Introduction section is a general discussion about the importance of viral gastroenteritis, types of viruses that cause gastroenteritis, and reasons for the combination of conventional diagnostic tests with biosensors for fast detection of viruses associated with gastroenteritis. Following the current laboratory detection tests for human gastroenteritis viruses and their limitations (with subsections: Electron Microscope (EM), Cell Culture, Immunoassay, and Molecular Techniques), structural features and significant aspects of various biosensing methods are discussed in the Biosensor section. In the next sections, basic information on viruses causing gastroenteritis and recent developments for fabrication and testing of different biosensors for each virus detection are covered, and the prospect of future developments in designing different biosensing platforms for gastroenteritis virus detection is discussed in the Conclusion and Future Directions section as well.

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“…SERS can be employed for direct spectroscopic characterisation, using non-functionalised nanostructured substrates. In 2001, Bao et al conducted a SERS study of insect nuclear polyhedrosis viruses in silver hydrogels [92] , while numerous other studies have used nanostructured metal substrates to study molecular signatures of, for example, respiratory viruses [93] , [94] , rotavirus [95] , [96] and poxviridae virions [97] . Fan et al demonstrated the use of SERS to detect and discriminate seven food and waterborne viruses in aqueous solution, including noro virus, adeno virus, parvo virus, rota virus, corona virus, paramyxo virus, and herpes virus [98] .…”

Section: Vibrational Spectroscopic Analysis Of Viruses and Viral Infe...mentioning

confidence: 99%

Contributions of vibrational spectroscopy to virology: A review

Chaudhary¹,

Jackson²,

Denning³

et al. 2022

Clinical Spectroscopy

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Octupolar Plasmonic Nanosensor Based on Ordered Arrays of Triangular Au Nanopillars for Selective Rotavirus Detection

Cited by 35 publications

References 60 publications

DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

Contributions of vibrational spectroscopy to virology: A review

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