Ocular and Systemic Safety Evaluation of Calcium Formate as a Dietary Supplement

Altaweel, Michael M.; Hanzlik, Robert P.; Hoeve, James N. Ver; Eells, Janis T.; Zhang, Baoyan

doi:10.1089/jop.2008.0128

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…In contrast, a few grams of formate per day are deemed safe [ 7 ]. A study supplementing healthy women with 1300 mg of calcium formate three times a day for 14 days reported no evidence for ocular toxicity [ 28 ]. In our study, we set the supplementation to 10% the daily one-carbon demand of mice.…”

Section: Discussionmentioning

confidence: 99%

Impact of Formate Supplementation on Body Weight and Plasma Amino Acids

et al. 2020

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Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM of sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology analyser. At the end of the study, blood and tissues were collected for metabolomics analysis and immune cell profiling. Formate supplementation had no significant physiological effect on male mice, except for a small decrease in body weight. Formate supplementation had no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice, however, the body weight and spleen wet weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of bifidobacteria, a probiotic bacterium, in the stools of female mice. We conclude that formate supplementation induces physiological changes in a gender-specific manner.

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Section: Discussionmentioning

confidence: 99%

Impact of Formate Supplementation on Body Weight and Plasma Amino Acids

et al. 2020

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“…In contrast, a few grams of formate per day are safe (7) . A study supplementing healthy women with 1,300 milligrams of calcium formate three times a day for 14 days reported no evidence for ocular toxicity (20) . We set the supplementation to 10% the daily one-carbon demand of mice.…”

Section: Discussionmentioning

confidence: 99%

Impact of formate supplementation on body weight and plasma amino acids

Dhayade

Pietzke

Wiesheu

et al. 2020

Preprint

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Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here we investigate the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology Analyser. At the end of the study blood and tissues were collected for metabolomics analysis and immune cell sorting. Formate supplementation has no significant physiological effect on male mice. Formate supplementation has no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice however, the body weight and spleen wet-weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of probiotic bacteria in the stools of female mice. We conclude that formate supplementation induces physiological changes in female mice.

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“…Calcium promotes blood clotting by activating the protein fibrin, and along with magnesium helps to regulate the heart beat, muscle tone, muscle contraction and nerve conduction. Ca 2+ supplementation is used in the treatment and prevention of osteoporosis in postmenopausal women (Reid et al , ; Altaweel et al , ; Sarr et al , , ). Furthermore, previous work demonstrated that Mg 2+ cations increased the statin passive diffusion (Sarr et al , , ).…”

Section: Introductionmentioning

confidence: 99%

H₂O₂: a Ca²⁺ or Mg²⁺‐sensing function in statin passive diffusion

Guillaume

Lethier

André

2015

Biomedical Chromatography

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In a previous paper Guillaume's group demonstrated that magnesium (Mg(2+) concentration range 0.00-2.60 mm) increased the passive diffusion of statins and thus played a role in their potential toxicity. In order to confirm an increase in this passive diffusion by divalent salt cations, the role of calcium chloride (CaCl2) on the statin-immobilized artificial membrane (IAM) association was studied. It was demonstrated that calcium supplementation (Ca(2+) concentration range 0.00-3.25 mm) increases the statin passive diffusion. In addition, it was shown that the Ca(2+) effect on the statin-IAM association is higher than that of Mg(2+). These results show that Ca(2+) enhances the passive diffusion of drugs into biological membranes and thus their potential toxicity. Also, addition of H2O2 to the medium showed a hyperbolic response for the statin passive diffusion and this effect was enhanced for the highest Ca(2+) or Mg(2+) concentrations in the medium. H2O2 is likely to interact with the polar head groups of the IAM through dipole-dipole interactions. The conformational changes in H2O2-IAM result in a higher degree of exposure of hydrophobic areas, thus explaining why the binding of pravastatin, which showed the lowest logP value, was less affected by H2O2. This result shows the significant contribution of H2O2 and thus the oxidative stress on the statin passive diffusion. Much of the sensitivity derives from the action of Ca(2+) or Mg(2+), in turn supported the idea that H2O2 may serve a Ca(2+) or Mg(2+) sensing function in statin passive diffusion.

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Ocular and Systemic Safety Evaluation of Calcium Formate as a Dietary Supplement

Cited by 5 publications

References 39 publications

Impact of Formate Supplementation on Body Weight and Plasma Amino Acids

Impact of Formate Supplementation on Body Weight and Plasma Amino Acids

Impact of formate supplementation on body weight and plasma amino acids

H₂O₂: a Ca²⁺ or Mg²⁺‐sensing function in statin passive diffusion

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Ocular and Systemic Safety Evaluation of Calcium Formate as a Dietary Supplement

Cited by 5 publications

References 39 publications

Impact of Formate Supplementation on Body Weight and Plasma Amino Acids

Impact of Formate Supplementation on Body Weight and Plasma Amino Acids

Impact of formate supplementation on body weight and plasma amino acids

H2O2: a Ca2+ or Mg2+‐sensing function in statin passive diffusion

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H₂O₂: a Ca²⁺ or Mg²⁺‐sensing function in statin passive diffusion