Ocular coherence tomography image data of the retinal laminar structure in a mouse model of oxygen-induced retinopathy

Dailey, Wendy; Drenser, Kimberly A.; Wong, Sui Chien; Cheng, Mei; Roumayah, Kevin; Feeney, Erin V.; Deshpande, Mrinalini; Guzmán, Alvaro; Trese, Michael T.; Mitton, Kenneth P.

doi:10.1016/j.dib.2017.09.075

Cited by 6 publications

(7 citation statements)

References 3 publications

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“…To view the entire span of the neural retina from the disc to the periphery, the stained sections were scanned using virtual microscopy (Dailey et al, 2017). While the progression of OIR induced laminar disorganization continued from age P21 to P42, some of the disorganization resolved by age P56 in both Norrin-treated and vehicle-treated OIR retinas (Dailey et al, 2017). Disruption in the laminar structure of the retina was most often located in the central half of the retina, and not the periphery, corresponding with the OIR-induced central avascular areas typical of the mouse OIR model.…”

Section: Resultsmentioning

confidence: 99%

“…Disruption in the laminar structure of the retina was most often located in the central half of the retina, and not the periphery, corresponding with the OIR-induced central avascular areas typical of the mouse OIR model. Disruption of the retinal layers involved displacement and mixing of photoreceptor nuclei with cell bodies of the inner nuclear retina (Dailey et al, 2017). …”

Section: Resultsmentioning

confidence: 99%

“…The disrupted architecture could partially resolve over several weeks but does persist. (Dailey et al, 2017). This is consistent with ERG observations, where significant decreases in the A-wave and B-wave amplitudes found in 4-week old OIR mice show partial recovery by 8-weeks (Nakamura et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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Norrin treatment improves ganglion cell survival in an oxygen-induced retinopathy model of retinal ischemia

Dailey

Drenser

Wong

et al. 2017

Experimental Eye Research

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Treatment of a mouse model of oxygen-induced retinopathy (OIR) with recombinant human Norrin (Norrie Disease Protein, gene: NDP) accelerates regrowth of the microvasculature into central ischemic regions of the neural retina, which are generated after treatment with 75% oxygen. While this reduces the average duration and severity of ischemia overall, we do not know if this accelerated recovery of the microvasculature results in any significant survival of retinal ganglion cells (RGCs). The purpose of this study was to investigate ganglion cell survival with and without the intravitreal injection of Norrin in the murine model of oxygen induced retinopathy (OIR), using two strains of mice: C57BL/6J and Thy1-YFP mice. Intravitreal injections of Norrin or vehicle were done after five days of exposure to 75% oxygen from ages P7 to P12. The C57BL/J mice were followed by Spectral-Domain Optical Coherence Tomography (SD-OCT), and the average nerve fiber layer (NFL) and inner-plexiform layer (IPL) thicknesses were measured at twenty-four locations per retina at P42. Additionally, some C57BL/J retinas were flat mounted and immunostained for the RGC marker, Brn3a, to compare the population density of surviving retinal ganglion cells. Using homozygous Thy1-YFP mice, single intrinsically fluorescent RGCs were imaged in live animals with a Micron-III imaging system at ages P21, 28 and P42. The relative percentage of YFP-fluorescent RGCs with dendritic arbors were compared. At age P42, the NFL was thicker in Norrin-injected OIR eyes, 14.4 μm, compared to Vehicle-injected OIR eyes, 13.3 μm (p=0.01). In the superior retina, the average thickness of the IPL was greater in Norrin-injected OIR eyes, 37.7 μm, compared to Vehicle-injected OIR eyes, 34.6 μm (p = 0.04). Retinas from Norrin injected OIR mice had significantly more surviving RGCs (p = 0.03) than vehicle-injected mice. Based upon NFL thickness and counts of RGCs, we conclude that Norrin treatment, early in the ischemic phase, increased the relative population density of surviving RGCs in the central retinas of OIR mice.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Norrin treatment improves ganglion cell survival in an oxygen-induced retinopathy model of retinal ischemia

Dailey

Drenser

Wong

et al. 2017

Experimental Eye Research

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“…In contrast, the tweezers group had a significant thicker total retina via SD-OCT measurements, but not after H&E staining at 8 days. This could be explained by the fact that disruption of the retina can be generated during dissection, embedding, cutting, and staining for H&E ( Dailey et al, 2017 ). Especially, processing the samples of the filter group could be worse through the attached filter.…”

Section: Discussionmentioning

confidence: 99%

Novel Porcine Retina Cultivation Techniques Provide Improved Photoreceptor Preservation

et al. 2020

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Age-related macular degeneration (AMD) is the leading cause of blindness in industrialized countries among people over 60 years. It has multiple triggers and risk factors, but despite intense research efforts, its pathomechanisms are currently not completely understood. AMD pathogenesis is characterized by soft drusen in Bruch’s membrane and involves the retinal pigment epithelium–Bruch’s membrane-choroid complex and adjacent structures, like photoreceptors. This study explores the potential of novel cultivation techniques to preserve photoreceptors in retinal explants to gain better insights in AMD pathology. The porcine retina explants were cultured for 4 and 8 days using three different explantation techniques, namely, control (photoreceptors facing down, touching the filter), filter (photoreceptors facing up, turned sample using a filter), and tweezers (photoreceptors facing up, turned sample using tweezers). Optical coherence tomography revealed that the tweezers method had the best capacity to limit thinning of the retinal explants. Both novel methods displayed advantages in maintaining outer segment thickness. Additionally, immunofluorescence evaluation revealed a better preservation of opsin + cells and rhodopsin signal intensity in both novel methods, especially the tweezers method. Furthermore, RT-qPCR analysis demonstrated an upregulation of OPSIN and RHODOPSIN mRNA expression in tweezers samples at 8 days. Amacrine and bipolar cell numbers were not altered at day 4 of cultivation, while cultivation until 8 days led to reduced bipolar cell numbers. At 4 days, CALRETININ mRNA was upregulated in filter samples, but protein kinase C alpha expression was downregulated. Retinal ganglion cells were diminished in both novel techniques due to a direct physical contact with the insert. Remarkably, no difference in TUBB3 mRNA expression was detected among the techniques. Nevertheless, both novel methods exhibited an improved retention of photoreceptor cells. In conclusion, the tweezers technique was the most promising one. Due to the high homology of the porcine to the human retina, it provides a reasonable alternative to in vivo rodent models. Consequently, an adapted coculture system based on the current findings may serve as an ex vivo model suitable to analyze AMD pathomechanisms and novel therapeutic approaches.

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“…Family members should also be screened, given that asymptomatic pathogenic features are commonly present, and asymptomatic family members are often identified. In the future, we may also be able to directly change the health and regeneration of human retinal endothelial cells through the application of Norrin protein, a therapy currently in development . Above all, patients and their family members must be informed that FEVR is a progressive lifelong disease that will require regular monitoring.…”

mentioning

confidence: 99%

Diagnosis and Management of Familial Exudative Vitreoretinopathy

2019

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Ocular coherence tomography image data of the retinal laminar structure in a mouse model of oxygen-induced retinopathy

Cited by 6 publications

References 3 publications

Norrin treatment improves ganglion cell survival in an oxygen-induced retinopathy model of retinal ischemia

Norrin treatment improves ganglion cell survival in an oxygen-induced retinopathy model of retinal ischemia

Novel Porcine Retina Cultivation Techniques Provide Improved Photoreceptor Preservation

Diagnosis and Management of Familial Exudative Vitreoretinopathy

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