Odontoblastic Differentiation, Inflammatory Response, and Angiogenic Potential of 4 Calcium Silicate–based Cements: Micromega MTA, ProRoot MTA, RetroMTA, and Experimental Calcium Silicate Cement

Chang, Seok Woo; Bae, Won Kyung; Yi, Jin-Kyu; Lee, Soo-Jung; Lee, Deok-Won; Kum, Kee‐Yeon; Kim, Eun-Cheol

doi:10.1016/j.joen.2015.04.018

Cited by 38 publications

(39 citation statements)

References 26 publications

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“…19−21 The results of the present study agree with previous descriptions of these biomaterials in terms of odontogenic activity. 21 Our results were also consistent with the study carried out by Chang et al, who reported similar ALP activity and up-regulation of odontoblastic markers such as ALP and DSPP between MTA and MM-MTA at 7 and 14 days. 32 In the present study, DSPP gene was significantly down-regulated after day 1 and again significantly up-regulated after day 7 by both biomaterials.…”

Section: Discussionsupporting

confidence: 93%

Odontogenic effects of two calcium silicate-based biomaterials in human dental pulp cells

et al. 2018

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Section: Discussionsupporting

confidence: 93%

Odontogenic effects of two calcium silicate-based biomaterials in human dental pulp cells

et al. 2018

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“…In vitro Angiogenesis Assay Kit (Bio-Rad) was used to test tube formation of HRECs according to the manufacturer's instruction [19]. Briefly, HRECs were seeded at 1.5 × 10 4 cells/well in 96-well plates pre-coated with matrigel.…”

Section: Angiogenesis Assaymentioning

confidence: 99%

MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy

Qin

Liu

et al. 2016

ABBS

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Diabetic retinopathy (DR) is the leading cause of new-onset blindness. The roles of microRNAs in diabetic retinopathy are largely unknown. The aim of this study is to investigate the role of miR20b in DR. Transfection of miR-20b mimic in high glucose (HG)-treated human retinal endothelial cells (HRECs) increased miR-20b expression and decreased the expression level of VEGF mRNA, while transfection of miR-20b inhibitor in control HRECs reduced the miR-20b expression with a corresponding increase of VEGF mRNA. In vitro functional assay showed that transfection of miR-20b mimic prevented HG-induced increase in transendothelial permeability and tube formation in HRECs. Transfection of miR-20b inhibitor or treatment of VEGF increased transendothelial permeability and tube formation in control HRECs. Luciferase reported assay showed that AKT3 is a target of miR-20b. Transfection of miR-20b mimic prevented the up-regulation of AKT3 induced by HG without changing the protein levels of other isoforms of AKT, and silencing of AKT3 caused decrease of VEGF mRNA and protein levels as well as prevented HG-induced increase in transendothelial permeability and tube formation. Finally, we showed that miR-20b was down-regulated in the retina and retinal endothelial cells in diabetic rats, with a correlated up-regulation of VEGF and AKT3. Intravitreal injection of miR-20b mimic in the diabetic rat significantly increased the miR-20b expression and decreased the expression levels of AKT3 and VEGF in the retina tissues, and intravitreal delivery of AKT3 siRNA in the diabetic rat significantly decreased the expressions of AKT3 and VEGF. Collectively, miR-20b is important for the regulation of VEGF-mediated changes in HRECs and rat retinal tissues under hyperglycemic conditions possibly via targeting AKT3.

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“…, Chang et al . ). In human pulp cell cultures placed in direct contact with MTA, vascular endothelial growth factor (VEGF) secretion increased by 30% compared to the level in cells grown without MTA (Paranjpe et al .…”

Section: Introductionmentioning

confidence: 97%

“…repair and pulp regeneration. ProRoot mineral trioxide aggregate (MTA; Dentsply Tulsa, Tulsa, OK, USA) has been applied successfully in various endodontic procedures; however, the possible angiogenic effect of this cement on surrounding cells and tissue has been examined only in a limited number of studies (Paranjpe et al 2011, Chou et al 2014, Chang et al 2015. In human pulp cell cultures placed in direct contact with MTA, vascular endothelial growth factor (VEGF) secretion increased by 30% compared to the level in cells grown without MTA (Paranjpe et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla

et al. 2015

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Odontoblastic Differentiation, Inflammatory Response, and Angiogenic Potential of 4 Calcium Silicate–based Cements: Micromega MTA, ProRoot MTA, RetroMTA, and Experimental Calcium Silicate Cement

Cited by 38 publications

References 26 publications

Odontogenic effects of two calcium silicate-based biomaterials in human dental pulp cells

Odontogenic effects of two calcium silicate-based biomaterials in human dental pulp cells

MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy

Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla

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