odr-10 Encodes a Seven Transmembrane Domain Olfactory Receptor Required for Responses to the Odorant Diacetyl

Abstract

Olfactory signaling is initiated by interactions between odorants and olfactory receptors. We show that the C. elegans odr-10 gene is likely to encode a receptor for the odorant diacetyl. odr-10 mutants have a specific defect in chemotaxis to diacetyl, one of several odorants detected by the AWA olfactory neurons. odr-10 encodes a predicted seven transmembrane domain receptor; a green fluorescent protein-tagged Odr-10 protein is localized to the AWA sensory cilia. odr-10 expression is regulated by odr-7, a transcription factor implicated in AWA sensory specification. Expression of odr-10 from a heterologous promoter directs behavioral responses to diacetyl, but not to another odorant detected by the AWA neurons. These results provide functional evidence for a specific interaction between an olfactory receptor protein and its odorant ligand.

ATP-2 Interacts with the PLAT Domain of LOV-1 and Is Involved inCaenorhabditis elegansPolycystin Signaling

“…ADPKD extrarenal manifestations include liver cysts and vascular -5(p813), and transgenic animals expressing Podr-10::ATP-2::GFP or Podr-10::atp-2-IR RNAi. Chemotaxis assays were performed as described in Sengupta et al (1996). Diacetyl was diluted 1:1000 in ethanol.…”

“…The AWA olfactory neurons sense attractive odorants, including diacetyl (Bargmann et al, 1993). The G protein-coupled odorant receptor ODR-10 is expressed and required in the complex, fork-shaped cilia of AWA neurons (Sengupta et al, 1996). To determine whether ATP-2 localizes to AWA cilia, we used the odr-10 promoter to drive the expression of ATP-2::GFP (Figure 8a) or ODR-10::GFP (Figure 8b).…”

Targeted gene alteration in Caenorhabditis elegans by gene conversion

“…Obtaining transposon-derived deletion alleles by the standard insertion-deletion approach seems to have been difficult, and some researchers have resorted to irradiation to encourage the formation of deletions after transposon excision 29 (M. Labouesse, personal communication). When we tested B200 populations of the otherwiseidentical nontransgenic mut-2; tkr-1 insertion strain by the standard Tc1 excision protocol, we isolated no germline deletions (data not shown).…”

Characterization of novel GPCR gene coding locus in amphioxus genome: Gene structure, expression, and phylogenetic analysis with implications for its involvement in chemoreception

“…Their gene structure contrasts sharply, however, with that of vertebrate ORs; their exons are interspersed among multiple introns (e.g., Robertson, 2000), and they show little sequence similarity with vertebrate olfactory receptors (Troemel et al, 1995;Sengupta et al, 1996;Clyne et al, 1999).…”