Oestrogen receptor alpha and beta in rat prostate and epididymis

Sar, Madhabananda; Welsch, Frank

doi:10.1046/j.1439-0272.2000.00396.x

Cited by 52 publications

(36 citation statements)

References 23 publications

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“…Testicular immunoexpression of ERa [28,29,49], ERb and AR [50,51] as well as seminal vesicle ERb and AR immunoexpression [30,52] and epididymal ERb [31,53] immunoexpression were all previously described and are consistent with what described in this study. A significant reduction in testicular ERb and ERb expression in pioglitazone-treated group as well as in epididymal and seminal vesicle ERb and AR expression in pioglitazone-and sitagliptin-treated groups were observed compared to the NC.…”

Section: Discussionsupporting

confidence: 90%

Impaired expression of sex hormone receptors in male reproductive organs of diabetic rat in response to oral antidiabetic drugs

Ayuob

Murad

Ali

2015

Folia Histochem Cytobiol.

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Introduction. Few oral antidiabetic drugs have been evaluated for their reproductive complication. This study aimed to evaluate the effect of metformin, pioglitazone and sitagliptin on the structure of male reproductive system through an immunohistopathological study. Material and methods. Sprague-Dawley male rats were injected with streptozotocin. The diabetic rats were divided into four groups (n = 8/each group); diabetic control, metformin-, pioglitazone-and sitagliptin-treated groups in addition to a normal control group (n = 8). At the end of the experiment, blood samples were collected for biochemical assessment. Testis, epididymis and seminal vesicle were dissected and processed for histopathological examination using routine and immune-staining. Results. All drugs significantly (p < 0.05) decreased fasting blood glucose, glycated hemoglobin, total cholesterol, triglycerides and malondialdehyde compared to the diabetic control group. Metformin has induced the least pathologic changes on the structure of the testis, epididymis and seminal vesicle among the studied drugs. Metformin succeeded to restore weights of testis, epididymis and seminal vesicle as well as testosterone hormone level back to values of the NC group while the pioglitazone and sitagliptin failed to do that. A significant reduction (p < 0.05) in testicular ERa and ERb immunoexpression of pioglitazone-treated group as well as suppression of ERb and AR immunoreactivity in in epididymus and seminal vesicles of pioglitazone-and sitagliptin-treated rats were observed compared to the control animals. Conclusions. Histological structure as well ER and AR expression in the system organs were negatively and significantly affected with all studied drugs. Metformin has the least effect on the structure of the studied male reproductive organs. Thus, pioglitazone and sitagliptin treatment should be avoided in young male diabetic patients.

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Section: Discussionsupporting

confidence: 90%

Impaired expression of sex hormone receptors in male reproductive organs of diabetic rat in response to oral antidiabetic drugs

Ayuob

Murad

Ali

2015

Folia Histochem Cytobiol.

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“…In the efferent ductules, small tubules transporting sperm from the rete testis to the caput epididymis, ERa is the predominant receptor subtype expressed (Hess et al 1997). In other reproductive organs of rats, like the epididymis, both the ERs are detectable, whereas in prostate ERb is the predominant form expressed (Sar & Welsch 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of estrogen receptor-subtype-specific ligands on fertility in adult male rats

Dumasia

Kumar

Kadam

et al. 2015

Journal of Endocrinology

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Maintenance of normal male fertility relies on the process of spermatogenesis which is under complex endocrine control by mechanisms involving gonadotropin and steroid hormones. Although testosterone is the primary sex steroid in males, estrogen is locally produced in the testis and plays a very crucial role in male fertility. This is evident from presence of both the estrogen receptors alpha (ERa) and beta (ERb) in the testis and their absence, as in the case of knockout mice models, leads to sterility. The present study was undertaken to understand individual roles of the two ERs in spermatogenesis and their direct contribution towards the maintenance of male fertility using receptor-subtype-specific ligands. Administration of ERa and b agonists to adult male rats for 60 days results in a significant decrease in fertility, mainly due to an increase in pre-and post-implantation loss and a concomitant decrease in litter size and sperm counts. Our results indicate that ERa is mainly involved in negative feedback regulation of gonadotropin hormones, whereas both ERs are involved in regulation of prolactin and testosterone production. Histological examinations of the testis reveal that ERb could be involved in the process of spermiation since many failed spermatids were observed in stages IX-XI following ERb agonist treatment. Our results indicate that overactivation of estrogen signaling through either of its receptors can have detrimental effects on the fertility parameters and that the two ERs have both overlapping and distinct roles in maintenance of male fertility.

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“…Both ER types are widely distributed in the male reproductive system, although ERb seems to be prevalent. Both ER types are present in the epididymis of pigs, rats and monkeys [9][10][11], however, in humans, ERb seems to be more prevalent [12]. The prostate in humans and rats contains mainly ERb, while ERa is rarely detectable [10,13].…”

Section: Introductionmentioning

confidence: 99%

Estrogen receptor alpha localization in the testes of men with normal spermatogenesis

Filipiak¹,

Suliborska²,

Walczak-Jędrzejowska³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:It is known that estrogens act on the male reproductive tract by binding to estrogen receptors (ER) a and b. However, studies on ER localization in the human testis are discordant. The aim of this study was to investigate the localization of ERa in the testes of adult men with normal spermatogenesis. Semen analysis of ten adult men revealed azoospermia. FSH, LH and testosterone serum concentrations were within normal values, and the volume of the testes was normal, hence obstructive azoospermia was suspected. The tissues from testicular surgical biopsies were fixed in Bouin's fluid and embedded in paraffin. Assessments of the seminiferous epithelium (scoring 10 to -1), the number of Leydig cells (scoring 1 to 5), the areal fraction of intertubular space (IS), measurements of seminiferous tubule diameter, and the thickness of the tubular wall, were performed on microscopic sections. Immunohistochemical staining was applied with monoclonal antibodies against ERa. The mean spermatogenesis score was 10 points; IS -30.6 ± 8.1%; seminiferous tubule diameter -193.9 ± 19.4 μm; thickness of tubular wall -7.44 ± 1.1 μm; number of Leydig cells -1.6 ± 1.1 points. Immunohistochemical staining showed the localization of ERa to be in the Sertoli and Leydig cell cytoplasm, while ERa was absent in germ cells. The results of testicular tissue analysis confirmed its normal structure and normal, full spermatogenesis. The presence of ERa in Sertoli and Leydig cells in normal human testis demonstrated in this study suggests that estrogens may affect testicular function.

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Oestrogen receptor alpha and beta in rat prostate and epididymis

Cited by 52 publications

References 23 publications

Impaired expression of sex hormone receptors in male reproductive organs of diabetic rat in response to oral antidiabetic drugs

Impaired expression of sex hormone receptors in male reproductive organs of diabetic rat in response to oral antidiabetic drugs

Effect of estrogen receptor-subtype-specific ligands on fertility in adult male rats

Estrogen receptor alpha localization in the testes of men with normal spermatogenesis

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