Oil biosynthesis and transcriptome profiles in developing endosperm and oil characteristic analyses in Paeonia ostii var. lishizhenii

Xiu, Yu; Wu, Guodong; Tang, Wensi; Peng, Zhengfeng; Bu, Xiangpan; Chao, Longjun; Yin, Xue; Xiong, Jing; Zhang, Haiwu; Zhao, Xiaoqing; Ding, Jing; Ma, Lvyi; Wang, Huafang; Staden, J. Van

doi:10.1016/j.jplph.2018.05.011

Cited by 26 publications

(21 citation statements)

References 75 publications

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“…Fan et al 53 reported that overexpression of oleosin promotes the clustering of small oil droplets in Arabidopsis seeds. The results of our current study indicate that 53–88 DAP is a period of rapid fatty acid biosynthesis in P. ostii seeds, which is consistent with the findings of Xiu et al 66 .…”

Section: Discussionsupporting

confidence: 93%

Integrated analysis of transcriptomic and proteomic data from tree peony (P. ostii) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism

et al. 2019

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Tree peony (Paeonia section Moutan DC.) seeds are an excellent source of beneficial natural compounds that promote health, and they contain high levels of alpha-linolenic acid (ALA). In recent years, tree peony has been emerging as an oil crop. Therefore, combined analysis of the transcriptome and proteome of tree peony (P. ostii) seeds at 25, 32, 39, 53, 67, 81, 88, 95, and 109 days after pollination (DAP) was conducted to better understand the transcriptional and translational regulation of seed development and oil biosynthesis. A total of 38,482 unigenes and 2841 proteins were identified. A total of 26,912 differentially expressed genes (DEGs) and 592 differentially expressed proteins (DEPs) were clustered into three groups corresponding to the rapid growth, seed inclusion enrichment and conversion, and late dehydration and mature stages of seed development. Fifteen lipid metabolism pathways were identified at both the transcriptome and proteome levels. Pathway enrichment analysis revealed that a period of rapid fatty acid biosynthesis occurred at 53–88 DAP. Furthermore, 211 genes and 35 proteins associated with the fatty acid metabolism pathway, 63 genes and 11 proteins associated with the biosynthesis of unsaturated fatty acids (UFAs), and 115 genes and 24 proteins associated with ALA metabolism were identified. Phylogenetic analysis revealed that 16 putative fatty acid desaturase (FAD)-encoding genes clustered into four FAD groups, eight of which exhibited the highest expression at 53 DAP, suggesting that they play an important role in ALA accumulation. RT-qPCR analysis indicated that the temporal expression patterns of oil biosynthesis genes were largely similar to the RNA-seq results. The expression patterns of fatty acid metabolism- and seed development-related proteins determined by MRM were also highly consistent with the results obtained in the proteomic analysis. Correlation analysis indicated significant differences in the number and abundance of DEGs and DEPs but a high level of consistency in expression patterns and metabolic pathways. The results of the present study represent the first combined transcriptomic and proteomic analysis of tree peony seeds and provide insight into tree peony seed development and oil accumulation.

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Section: Discussionsupporting

confidence: 93%

Integrated analysis of transcriptomic and proteomic data from tree peony (P. ostii) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism

et al. 2019

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“…Transcriptomes have previously been used to study the mechanisms underlying ALA synthesis in tree peony seeds [10][11][12][13][14]. Here, we identified genes associated with the lipid metabolism that were differentially expressed during the development of the seed kernel, testa, and pericarp.…”

Section: Discussionmentioning

confidence: 99%

“…There are nine wild species in section Moutan DC, which differ with respect to preferred habitat, but all rich in unsaturated FAs (>90%) and ALA (26.7-50%) [6,9]. To date, transcriptomic, proteomic, and microRNA sequencing have been used to investigate the mechanisms underlying ALA synthesis in tree peony seeds [10][11][12][13][14]. Previous studies have focused on enzymes that are key to ALA biosynthesis in various tree peony species, including FAD3 in P. rockii, P. potaninii and P. lutea seed [15]; FAD2 and FAD8 in P. ostii [10]; and ACCase, FATA, LPCAT, FADs, and DGAT in the developing endosperm of P. ostii var.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have focused on enzymes that are key to ALA biosynthesis in various tree peony species, including FAD3 in P. rockii, P. potaninii and P. lutea seed [15]; FAD2 and FAD8 in P. ostii [10]; and ACCase, FATA, LPCAT, FADs, and DGAT in the developing endosperm of P. ostii var. lishizhenii [11]. Wang et al [14] identified 115 genes and 24 proteins associated with the ALA metabolism in P. ostii seeds.…”

Section: Introductionmentioning

confidence: 99%

“…Zhang et al [13,15] compared FAD transcription (i.e., SAD, FAD2, and FAD3) among three species of tree peonies, and found that FAD transcription was greater in P. rockii seeds than in P. potaninii and P. lutea seeds. In addition, various transcriptomes of P. ostii seeds or endosperms are also available [10,11,14]. However, to date, no integrated analysis of the developing pericarp, seed coat, and endosperm in P. ostii has been performed.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of a-linolenic acid content and biosynthesis in Paeonia ostii fruits and seeds

Zhang

et al. 2020

Preprint

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Background Paeonia ostii is a potentially important oilseed crop because its seed yield is high and the seeds are rich in α-linolenic acid (ALA). However, the molecular mechanisms underlying ALA biosynthesis during seed kernel, seed testa, and fruit pericarp development in this plant are unclear. We used transcriptome data to address this knowledge gap. Results Gas chromatograph-mass spectrometry indicated that ALA content was highest in the kernel, moderate in the testa, and lowest in the pericarp. Therefore, we used RNA-sequencing to compare ALA synthesis among these three tissues. We identified 227,837 unigenes, with an average length of 755 bp. Of these, 1371 unigenes were associated with lipid metabolism. The fatty acid (FA) biosynthesis and metabolism pathways were significantly enriched during the early stages of oil accumulation in the kernel. ALA biosynthesis was significantly enriched in parallel with increasing ALA content in the testa, but these metabolic pathways were not significantly enriched during pericarp development. By comparing unigene transcription profiles with patterns of ALA accumulation, specific unigenes encoding crucial enzymes and transcription factors (TFs) involved in de novo FA biosynthesis and oil accumulation were identified. Specifically, the bell-shaped expression patterns of genes encoding SAD, FAD2, FAD3, PDCT, PDAT, OLE, CLE, and SLE in the kernel were similar to the patterns of ALA accumulation in this tissue. Genes encoding BCCP, BC, KAS I– III, and FATA were also upregulated during the early stages of oil accumulation in the kernel. In the testa, the upregulation of the genes encoding SAD, FAD2, and FAD3 was followed by a sharp increase in the concentrations of ALA. In contrast, these genes were minimally expressed (and ALA content was low) throughout pericarp development. Conclusions In this study, we used three tissues with high, moderate, and low ALA concentrations as an exemplar system in which to compare tissue-specific ALA accumulation mechanism in P. ostii. The genes and TFs identified herein might be useful targets for future studies of ALA accumulation in the tree peony. This study also provides a framework for future studies of FA biosynthesis in other oilseed plants.

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Expression profiles of genes involved in fatty acid and lipid biosynthesis in developing seeds of Paeonia ostii

Que

et al. 2021

Genes Genom

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Oil biosynthesis and transcriptome profiles in developing endosperm and oil characteristic analyses in Paeonia ostii var. lishizhenii

Cited by 26 publications

References 75 publications

Integrated analysis of transcriptomic and proteomic data from tree peony (P. ostii) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism

Integrated analysis of transcriptomic and proteomic data from tree peony (P. ostii) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism

Transcriptomic analysis of a-linolenic acid content and biosynthesis in Paeonia ostii fruits and seeds

Expression profiles of genes involved in fatty acid and lipid biosynthesis in developing seeds of Paeonia ostii

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