Okadaic acid: a potential defense molecule for the sponge Suberites domuncula

Wiens, Matthias; Luckas, Bernd; Brümmer, Franz; Shokry, M.; Ammar, Achraf; Steffen, Renate; Batel, Renato; Diehl‐Seifert, Bärbel; Schröder, Heinz C.; Müller, Werner

doi:10.1007/s00227-002-0886-6

Cited by 27 publications

(42 citation statements)

References 47 publications

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“…leukemia cells (Nervi et al, 1998). In a recent study it was established that in the sponge system caspases are also involved in the induction of apoptosis (Wiens et al, 2003). Surprisingly it was found that in the presence of retinoic acid the expression level of the caspase gene does not alter, and this finding was also supported by a series of experiments which showed that there is no parallel increase in the formation of free nucleosomes during the retinoic acid-mediated regression of tissue.…”

Section: Discussionmentioning

confidence: 92%

“…We investigated whether the morphogenetic effect of retinoic acid in the primmorph system occurs via caspasemediated apoptosis or via a controlled expression of homeotic genes, by studying expression of the S. domuncula caspase gene (SDCASPR; accession number AJ426651; Wiens et al, 2003a) and the LIM/homeobox protein (SDLIM4; AJ493059) in parallel. The S. domuncula caspase is also involved in apoptosis (Wiens et al, 2003).…”

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confidence: 99%

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Retinoid X receptor and retinoic acid response in the marine sponge Suberites domuncula

Wiens

Batel

Korzhev

et al. 2003

Journal of Experimental Biology

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SUMMARY To date no nuclear receptors have been identified or cloned from the phylogenetically oldest metazoan phylum, the Porifera (sponges). We show that retinoic acid causes tissue regression in intact individuals of the demosponge Suberites domuncula and in primmorphs, special three-dimensional cell aggregates. Primmorphs were cultivated on a galectin/poly-L-lysine matrix in order to induce canal formation. In the presence of 1 or 50 μmol l–1 retinoic acid these canals undergo regression, a process that is reversible. We also cloned the cDNA from S. domunculaencoding the retinoid X receptor (RXR), which displays the two motifs of nuclear hormone receptors, the ligand-binding and the DNA-binding domains, and performed phylogenetic analyses of this receptor. RXR expression undergoes strong upregulation in response to treatment with retinoic acid, whereas the expression of the sponge caspase is not increased. The gene encoding the LIM homeodomain protein was found to be strongly upregulated in response to retinoic acid treatment. These data indicate that the RXR and its ligand retinoic acid play a role in the control of morphogenetic events in sponges.

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Section: Discussionmentioning

confidence: 92%

mentioning

confidence: 99%

Retinoid X receptor and retinoic acid response in the marine sponge Suberites domuncula

Wiens

Batel

Korzhev

et al. 2003

Journal of Experimental Biology

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“…No reports have identified the biosynthetic pathway of any secondary metabolite of dinoflagellate origin at the genomic level (Snyder et al, 2005). Although immunological evidence suggests that bacteria inside the sponge Suberites dumuncula as well as sponge cells and intracellular vacuoles all contain OA (Wiens et al, 2003;Schröder et al, 2006), no bacteria that produce a polyketide toxin have been isolated from a dinoflagellate (Wiens et al, 2003;Snyder et al, 2005).…”

Section: Okadaic Acidmentioning

confidence: 99%

“…Studies by Wiens et al (2003) have provided evidence for at least two putative roles of OA within the sponge Suberites domuncula. At low concentrations (Ͻ100 nmol l -1 ), OA triggers a MAP kinase p38-regulated defense system against bacteria.…”

Section: Okadaic Acidmentioning

confidence: 99%

Chemical Defenses: From Compounds to Communities

Paul

Arthur

Ritson‐Williams

et al. 2007

The Biological Bulletin

180

152

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“…Since the rate of predation is higher in temperate habitats, tropical sponges have evolved more effective secondary metabolites to deter predators (Becerro et al, 2003). Natural spatiotemporal variations of secondary metabolites from marine sponges have been reported in several papers (Page et al, 2005;Abdo et al, 2007), however, only fewer studies observed correlations between fluctuations of isolated bioactive compounds and environmental stressors in their habitats (Thompson et al, 1987;Wiens et al, 2003;Abdo et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides

Dewi

Hadi²,

Januar³

et al. 2013

Squalen Bull. Marine Fisheries Postharvest Biotech.

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This experiment was conducted to study the effects of anthropogenic stressor on the spatial variability of secondary metabolites from marine sponge Aaptos suberitoides. Samplings were conducted at 7 sites in Marine National Park of Thousand Islands that are extended within 30 km off Jakarta bay on late February 2011. Sponges were collected and quantified by means of liquid chromatography coupled with photo-diode array detection, whereas, cytotoxicity of sponges extracts was determined against T47D (breast) cancer cell lines. Results showed that the spatial production of aaptamine and isoaaptamine did not correlate with the quality of their surrounding habitat, despite nitrite and nitrate levels significantly affected the bioactivity of crude extracts.Keywords: A. suberitoides, aaptamines, spatial production, cytotoxicity, pollutants ABSTRAK Penelitian ini dilakukan untuk mempelajari efek tekanan antropogenik terhadap variabilitas spasial metabolit sekunder dari spons laut A. suberitoides. Kegiatan sampling dilakukan di tujuh titik yang berada di area Taman Nasional Kepulauan Seribu yang tersebar sepanjang 30 km dari wilayah Teluk Jakarta pada bulan Februari 2011. Spons dikoleksi dan dikuantifikasi menggunakan kromatografi cair yang dilengkapi deteksi photo-diode array sedangkan sitotoksisitas ekstrak spons diukur terhadap sel kanker T47D (payudara). Hasil penelitian menunjukkan bahwa produksi spasial dari aaptamin dan isoaaptamin tidak berkorelasi dengan kualitas dari habitat disekitarnya, meskipun kadar nitrit dan nitrat mempengaruhi bioaktivitas ekstrak pekat secara signifikan.

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Okadaic acid: a potential defense molecule for the sponge Suberites domuncula

Cited by 27 publications

References 47 publications

Retinoid X receptor and retinoic acid response in the marine sponge Suberites domuncula

Retinoid X receptor and retinoic acid response in the marine sponge Suberites domuncula

Chemical Defenses: From Compounds to Communities

STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides

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