OL‐protocadherin expression in the visual system of the chicken embryo

Abstract

The expression of OL-protocadherin, a homotypically binding cell adhesion molecule, was mapped in the visual system of the chicken embryo at intermediate to late stages of development (11-19 days of incubation). The expression was compared with that of four classic cadherins, described previously. OL-protocadherin is expressed by the isthmooptic nucleus, its retinopetal projection, and possibly its retinal target neurons, the amacrine cells. Ganglion cells begin to express OL-protocadherin at relatively late stages of development. The layers of the optic tectum, the projection neurons in the stratum griseum centrale, and the tectofugal pathways show differential OL-protocadherin immunoreactivity. Several of the diencephalic target nuclei of the tectothalamic projection, such as the principal pretectal nucleus, subpretectal nucleus, and nucleus rotundus, contain distinct subregions or populations of neurons expressing OL-protocadherin. In these centers, the expression pattern of OL-protocadherin differs from that of the four classic cadherins, though it shows partial overlap with them. Other retinorecipient and/or tectorecipient nuclei (ventral geniculate nucleus, lateral dorsolateral nucleus, superficial synencephalic nucleus, pretectal area, and griseum tectale) also show a differential immunoreactivity for OL-protocadherin and other cadherins. Some of these nuclei and the optic tectum display a similar sequence of cadherin expression from superficial to deep layers, in a pattern that may reflect mutual interconnections. This result indicates a partial conservation of cadherin expression across interconnected embryonic divisions, from the mesencephalon to the ventral thalamus. In conclusion, OL-protocadherin is a marker for specific functional gray matter structures and neural circuits in the chicken visual system. J. Comp. Neurol. 470:240-255, 2004.

Expression of delta‐protocadherins in the spinal cord of the chicken embryo

“…In the vertebrate brain, Pcdh10 is expressed in neural circuits of several functional systems (Hirano et al, 1999;Müller et al, 2004). Our results confirm findings by Nakao et al (2005).…”

“…In addition, the following plasmids were used for probe synthesis: OL-protocadherin (nucleotides 70-352 and 2,259-3,899 from the cDNA of chicken Pcdh10; Genbank accession no. AF334802; Müller et al, 2004;Nakao et al, 2005; gift of Dr. S. Hirano, RIKEN for Developmental Biology, Kobe, Japan); Cadherin-7 (full-length sequence of Cdh7, Genbank accession no. NM_204187.2; Nakagawa and Takeichi, 1995; gift of Drs.…”

Embryonic genoarchitecture of the pretectum in Xenopus laevis: A conserved pattern in tetrapods

“…Two recent studies also supported this hypothesis for amphibians (Milán and Puelles, 2000;Morona and González, 2008), and substantial evidence has been reported for amniote tetrapods (Martínez-de-la-Torre, 1985;Medina et al, 1992Medina et al, , 1993Puelles, 1995Puelles, , 2001Redies et al, 1997Redies et al, , 2000De Castro et al, 1998;Dávila et al, 2000;Martínez and Puelles, 2000;Yoon et al, 2000;GarciaCalero et al, 2002;Puelles and Rubenstein, 2003;Müller et al, 2004;García-López et al, 2004;Ferran et al, 2007Ferran et al, , 2009). Thus, the data presented here confirm the suggested subdivisions in amphibians (Puelles et al 1996;Milán and Puelles, 2000;Morona and González, 2008) and corroborate the model proposed for amniotes (Ferran et al, 2007(Ferran et al, , 2008.…”

High-resolution melting analysis of PCDH10 methylation levels in gastric, colorectal and pancreatic cancers

“…protocadherin 10 (PCDH10/OL-PCDH/KIAA1400) is a nonclustered-type protocadherin with a unique cytoplasmic domain [5]. It is reported that there is a correlation between the expression pattern of PCDH10 and functional systems of the brain such as the visual and limbic systems [6][7][8]. Unlike other protocadherin family members, PCDH10 was discovered to have widespread expression [9].…”