Old antibiotics for multidrug-resistant pathogens: from in vitro activity to clinical outcomes

Kaye, Keith S.; Gales, Ana Cristina; Dubourg, G

doi:10.1016/j.ijantimicag.2016.11.020

Cited by 33 publications

(15 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, aminopenicillins cannot be administered in case of allergy to β-lactams, and gentamicin has a poor intracellular penetration, does not cross the BBB efficiently, and is nephrotoxic or ototoxic [10, 69, 70]. Due to its bactericidal activity and synergistic action with many antimicrobials including β-lactams, aminoglycosides, meropenem, linezolid, daptomycin and vancomycin, the “old” antibiotic fosfomycin [14] is currently resurging as a therapeutic option for the treatment of critically ill patients with invasive or systemic bacterial infections [12, 13, 71]. Rapid, effective bactericidal action is paramount in neuromeningeal, bacteremic or materno-fetal listeriosis to limit disease severity, relapses and fatal outcomes [2, 9, 70, 72, 73].…”

Section: Discussionmentioning

confidence: 99%

Epistatic control of intrinsic resistance by virulence genes in Listeria

et al. 2018

View full text Add to dashboard Cite

Elucidating the relationships between antimicrobial resistance and virulence is key to understanding the evolution and population dynamics of resistant pathogens. Here, we show that the susceptibility of the gram-positive bacterium Listeria monocytogenes to the antibiotic fosfomycin is a complex trait involving interactions between resistance and virulence genes and the environment. We found that a FosX enzyme encoded in the listerial core genome confers intrinsic fosfomycin resistance to both pathogenic and non-pathogenic Listeria spp. However, in the genomic context of the pathogenic L. monocytogenes, FosX-mediated resistance is epistatically suppressed by two members of the PrfA virulence regulon, hpt and prfA, which upon activation by host signals induce increased fosfomycin influx into the bacterial cell. Consequently, in infection conditions, most L. monocytogenes isolates become susceptible to fosfomycin despite possessing a gene that confers high-level resistance to the drug. Our study establishes the molecular basis of an epistatic interaction between virulence and resistance genes controlling bacterial susceptibility to an antibiotic. The reported findings provide the rationale for the introduction of fosfomycin in the treatment of Listeria infections even though these bacteria are intrinsically resistant to the antibiotic in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

Epistatic control of intrinsic resistance by virulence genes in Listeria

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In France, it is the only first-line antimicrobial drug recommended for treatment of cystitis (97% susceptibility) and is used in 20%–30% of such treatments ( 2 ). However, it is receiving renewed worldwide attention as one of the most active agents for sparing carbapenems in extended spectrum β-lactamase (ESBL)–producing isolates and for treatment of carbapeneme-resistant Enterobacteriaceae (CRE) in combination with colistin ( 3 ). In France, intravenous fosfomycin (3–4 g 4×/d) is used in combination with other drugs for the treatment of multidrug-resistant infections.…”

mentioning

confidence: 99%

Emergence of Plasmid-Mediated Fosfomycin-Resistance Genes among Escherichia coli Isolates, France

Benzerara¹,

Gallah²,

Hommeril³

et al. 2017

Emerg. Infect. Dis.

View full text Add to dashboard Cite

show abstract

“…Antibiotics and germicides effectively control infective pathogens and have been widely applied in agricultural production [39]. However, drug resistance accompanies the use of antibiotics and germicides.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Antioxidant and antimicrobial properties ofIsodon amethystoides(Benth.) CY Wu et Hsuan leaf extracts against agriculturally important pathogenic fungi

Zhao

Zhang

Xue

et al. 2019

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

In this study, we investigated the antioxidant and antimicrobial activities of Isodon amethystoides (Benth.) CY Wu et Hsuan leaf extracts. Leaves were subjected to extraction with acetone, chloroform, ethanol and ethyl acetate. The extraction efficiency of these four solvents ranked as follows: acetone > chloroform > ethanol > ethyl acetate; highest extraction yield of 18.27% was obtained using acetone as the solvent. Total phenolic content and flavonoid content were determined using Folin-Ciocalteau and aluminium chloride (AlCl 3) methods, respectively. The acetone extract had the highest total phenolic content (146.77 mg GAE/g dry extract) and flavonoid content (81.57 mg RE/g dry extract) among the four types of extracts. Further, the antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the antimicrobial activity was evaluated using disc-diffusion and broth microdilution assay. The strongest antioxidant activity was observed in the acetone extract with a half minimal inhibitory concentration (IC 50) of 0.4024 mg/mL in the DPPH scavenging assay. The acetone and ethyl acetate extracts exhibited strong antimicrobial activity. The strongest antibacterial activity of the acetone extract was observed against Bacillus subtilis and Escherichia coli with a minimum inhibitory concentration (MIC) of 0.10 mg/mL and 0.05 mg/mL, respectively. The acetone extract had high antifungal activity against three of the six agriculturally important pathogenic fungi with MIC ranging from 0.5 mg/mL to 0.8 mg/mL. The antioxidant and antimicrobial activities may be attributed to the high total phenolic and flavonoid contents in the acetone extract. Our findings suggest I. amethystoides leaves as an important source of natural compounds with potential use in agriculture.

show abstract

Old antibiotics for multidrug-resistant pathogens: from in vitro activity to clinical outcomes

Cited by 33 publications

References 51 publications

Epistatic control of intrinsic resistance by virulence genes in Listeria

Epistatic control of intrinsic resistance by virulence genes in Listeria

Emergence of Plasmid-Mediated Fosfomycin-Resistance Genes among Escherichia coli Isolates, France

Antioxidant and antimicrobial properties ofIsodon amethystoides(Benth.) CY Wu et Hsuan leaf extracts against agriculturally important pathogenic fungi

Contact Info

Product

Resources

About