Oleaginous yeast biomass flocculation using bioflocculant produced in wastewater sludge and transesterification using petroleum diesel as a co-solvent

Yellapu, Sravan Kumar; Klai, Nouha; Kaur, Rajwinder; Tyagi, Rajeshwar Dayal; Surampalli, Rao Y.

doi:10.1016/j.renene.2018.06.066

Cited by 34 publications

(27 citation statements)

References 30 publications

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“…The fermentation section is similar to that described above. Fermentation was followed by biomass settling which was performed using 70 mM calcium chloride followed by doses of EPS (119 mg/g biomass) (Yellapu et al, 2018b) (Fig. 2).…”

Section: Microbial Lipid Production Using Pure Substrate (Glucose Andmentioning

confidence: 99%

“…2). The concentration of EPS and calcium chloride is dependent on the biomass concentration (more than 60 g/L biomass) obtained in fermented broth (Yellapu et al, 2018b). The settled biomass (166 g/L) was treated by N-lauryl sarcosine (40 mg N-LS/g biomass) (Yellapu et al, 2018b).…”

Section: Microbial Lipid Production Using Pure Substrate (Glucose Andmentioning

confidence: 99%

“…The concentration of EPS and calcium chloride is dependent on the biomass concentration (more than 60 g/L biomass) obtained in fermented broth (Yellapu et al, 2018b). The settled biomass (166 g/L) was treated by N-lauryl sarcosine (40 mg N-LS/g biomass) (Yellapu et al, 2018b). The lipid was recovered using petroleum-diesel (PD) as solvent (14.33 mL PD/g lipid) where the N-LS treated biomass was treated with PD at 70 °C for 20 min (Yellapu et al, 2018b).…”

Section: Microbial Lipid Production Using Pure Substrate (Glucose Andmentioning

confidence: 99%

“…Recently in INRS lab, biomass settling has been performed using chemical coagulant, Calcium chloride and bio-flocculant (extra-polymeric substances or EPS) that will eliminate the requirement of centrifugation. The cell wall disruption was successfully performed by utilizing free nitrous acid or bio-surfactant n-lauryl sarcosine (Yellapu et al, 2016;Yellapu et al, 2018b). The released lipid after cell wall disruption was separated by phase separation with the aid of petroleum-diesel as solvent, which eliminated the requirement of organic solvents like chloroform, methanol and hexane (Yellapu et al, 2018b).…”

mentioning

confidence: 99%

“…The cell wall disruption was successfully performed by utilizing free nitrous acid or bio-surfactant n-lauryl sarcosine (Yellapu et al, 2016;Yellapu et al, 2018b). The released lipid after cell wall disruption was separated by phase separation with the aid of petroleum-diesel as solvent, which eliminated the requirement of organic solvents like chloroform, methanol and hexane (Yellapu et al, 2018b). Moreover, there will be no requirement of blending of petroleum diesel with biodiesel.…”

mentioning

confidence: 99%

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Energy balance for biodiesel production processes using microbial oil and scum

Kumar

Yellapu

Zhang

et al. 2019

Bioresource Technology

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Continuous use of fossil fuels is the major contributor of greenhouse gas emissions, which requires developing the alternate green sources of energy that should be sustainable and environmentally friendly. Biodiesel, fatty acid methyl esters (FAMEs), has grabbed great attention due to the advantages such as: it is renewable, sustainable, environmentally friendly (burns much cleaner than petroleum diesel), compatible with current commercial diesel engines, as well as has excellent lubricity and could provide energy density similar to diesel. As a feasible energy source, biodiesel production should be a sustainable and energetically favourable process (the difference between the energy output and the energy input is positive) (Thamsiriroj and Murphy, 2011; Zhang et al., 2013). Current biodiesel feedstock like agriculture, fossils and wood cause environmental concerns like global warming, greenhouse gas emission and also leads to fast depletion of natural resources like deforestation. The soaring price of edible oil leads to biodiesel production unaffordable and present feedstock sources for fuel production. Moreover, some researchers have reported that biodiesel produced from two major raw materials, soybean and sunflower oils, was energetically unfavorable due to the low oil yield of the crops (energy loss of 32% for soybean and 118% for sunflower) (Pimentel and Patzek, 2005). Therefore, it has forced the researchers and engineers to search for replacement of the traditional oils and lipids as raw materials, which should be abundant, sustainable, and energetically favorable (positive energy balance). Oleaginous microorganisms have shown a great advantage as lipid source due to their faster growth rate and high lipid contents (up to 80% microorganism dry weight) compared to oilseed crops and animals (Gouda et al., 2008). However, techno-economic evaluation of microbial oil production using glucose as media revealed that unit production cost of biodiesel using microbial oil production with glucose as substrate was estimated to be $5.9/kg while commercial biodiesel price was $1.2/L (Apostolakou et al., 2009). Since biodiesel with microbial oil technology with glucose as substrate is an expensive process, alternatives for inexpensive sources should be looked upon. Recently biodiesel production has been reported using renewable waste sources such as crude glycerol and municipal sludge (

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Section: Microbial Lipid Production Using Pure Substrate (Glucose Andmentioning

confidence: 99%

Section: Microbial Lipid Production Using Pure Substrate (Glucose Andmentioning

confidence: 99%