Oleanolic acid inhibits the activity of the multidrug resistance protein ABCC1 (MRP1) but not of the ABCB1 (P-glycoprotein): Possible use in cancer chemotherapy

Braga, Filipe; Ayres-Saraiva, D.; Gattass, Cerli Rocha; Capella, Marcia A. M.

doi:10.1016/j.canlet.2006.06.008

Cited by 53 publications

(41 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the effect of oleanolic acid on the activity of MDR proteins has been investigated. Although it did not interfere with ABCB1 activity, oleanolic acid did modulate ABCC1 activity (20).…”

Section: Resultsmentioning

confidence: 78%

“…Therefore, the identification of new anti-neoplastic drugs that can bypass resistance and do not modulate the activity or alter the expression of the two main MDR proteins is of great importance. Our group is studying the anti-tumoral activity of several triterpenes of natural origin, and we have shown that several are cytotoxic for MDR cell lines (20,24,(26)(27)(28). Additionally, the effect of oleanolic acid on the activity of MDR proteins has been investigated.…”

Section: Resultsmentioning

confidence: 99%

“…The antitumoral effect of BA was also observed in tumor cell lines with defective or mutated p53 (13,16), as well as in cell lines that acquire resistance to cytotoxic drugs (16,18). In this study, we investigated the effect of BA on Ma-104, a non-tumoral renal cell line that constitutively expresses P-gp/ABCB1 and MRP1/ABCC1 (20). The results show that, at lower concentrations (≤10 μg/ml), the cytotoxic effects of BA on Ma-104 cells are much smaller than those observed in tumor cell lines (14,16,19,29), including those resistant to cytotoxic drugs (16,30).…”

Section: Resultsmentioning

confidence: 99%

“…Later, it was demonstrated that BA is also active in other types of tumors, including colon and prostate (15), lung, ovarian, cervical (16), leukemia (17), liver (18), and head and neck (19) carcinomas. Previous research by our group revealed that BA was cytotoxic to an MDR erythroleukemic cell line overexpressing P-glycoprotein, even in the absence of MDR modulators (20). This observation aroused interest in the further characterization of the cytotoxicity of this triterpene, particularly in non-tumoral cell lines.…”

Section: Introductionmentioning

confidence: 93%

“…Due to the growing interest in new compounds capable of overcoming the MDR phenotype for clinical praxis, the present study was performed to examine the effects of BA on the activity of ABCB1 and ABCC1 proteins in a non-tumoral renal cell line constitutively expressing both proteins (20). The results obtained indicate that BA had no effect on the activity or expression of these proteins in the cell line, and that it did not interact with a chemotherapic agent, methotrexate.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Betulinic acid does not modulate the activity of P-gp/ABCB1 or MRP1/ABCC1 in a non-tumoral renal cell line: Possible utility in multidrug resistance cancer chemotherapy

Delou

Capella²,

Gattass³

2009

Mol Med Rep

View full text Add to dashboard Cite

Abstract. Multidrug resistance (MDR) is a multifactorial phenomenon considered to be the main cause of failure in cancer chemotherapy. One of the underlying mechanisms of MDR is the overexpression of membrane transporter proteins, such as P-glycoprotein (P-gp/ABCB1) and multidrug resistance-associated protein 1 (MRP1/ABCC1). As these proteins are also expressed in normal tissues, considerable attention has been dedicated to the search for cytotoxic drugs that are not substrates for these proteins. This study investigated the effects of betulinic acid (BA) on the activity of ABCB1 and ABCC1 in Ma-104, a non-tumoral renal cell line constitutively expressing both proteins. The results indicated that concentrations of BA with low cytotoxicity to Ma-104 did not alter the activity of ABCB1 or ABCC1, nor did BA interfere with the accumulation of a classic chemotherapeutic, methotrexate. This suggests it would also be a good choice for use in drug cocktails. The lack of effect of BA on ABCB1 and ABCC1, as well as its antitumoral properties, suggest that this triterpene is a viable chemotherapeutic agent for MDR tumors.

show abstract

Section: Resultsmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Betulinic acid does not modulate the activity of P-gp/ABCB1 or MRP1/ABCC1 in a non-tumoral renal cell line: Possible utility in multidrug resistance cancer chemotherapy

Delou

Capella²,

Gattass³

2009

Mol Med Rep

View full text Add to dashboard Cite

show abstract

Enrichment of pomace olive oil in triterpenic acids during storage of “Alpeorujo” olive paste

García

Brenes

Dobarganes

et al. 2008

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

Triterpenic acids are natural compounds present in plants and foods with beneficial properties for human health and thus they are desirable in the food, cosmetics and pharmaceutical industries. Pomace olive is considered a good source of these substances. In this study, oleanolic and maslinic acids were found to be the main triterpenic acids identified in pomace olive oil obtained from stored "Alpeorujo". Determination of the two acids was achieved by a new procedure that consists of extracting the acids from the oil with a mixture of methanol/ethanol, and then separating and quantifying them by HPLC. Results showed that their concentration increased up to 16 g/kg of oil during storage of the pomace in large ponds for 7 months. The concentration of both triterpenic acids was similar in the pomace olive oil obtained by using the centrifugation system. By contrast, a much lower concentration of maslinic than oleanolic acid was detected in pomace olive oils obtained by solvent extraction from the previously centrifugated "Alpeorujo" paste. These triterpenic acids also contributed to the acidity of the crude oil. Likewise, the oil of the pomace paste was enriched in other substances such as 4-ethylphenol and aliphatic alcohols during the storage of the paste in large ponds. Consequently, crude pomace olive oil can be considered a good source of triterpenic acids when obtained from a stored olive paste.

show abstract

Oxidation of DNA Bases Influenced by the Presence of Other Bases

Goh

Pumera

2012

Electroanalysis

View full text Add to dashboard Cite

Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak width and resolution can be highly influenced by the presence of other DNA bases at electrochemically reduced graphene oxide (ER‐GO) as well as standard glassy carbon electrode. We have observed that the effects were more significant for adenine base on ER‐GO and cytosine base on glassy carbon (GC) electrode. Differences in responses were generally low in a mixture of four different DNA bases but interestingly, deviations become significantly larger when only one or two other bases were present. Our findings are of paramount importance for future developments in DNA detection and analysis since individual DNA bases are not present in isolation in nature or in typical biosensing systems.

show abstract

Oleanolic acid inhibits the activity of the multidrug resistance protein ABCC1 (MRP1) but not of the ABCB1 (P-glycoprotein): Possible use in cancer chemotherapy

Cited by 53 publications

References 23 publications

Betulinic acid does not modulate the activity of P-gp/ABCB1 or MRP1/ABCC1 in a non-tumoral renal cell line: Possible utility in multidrug resistance cancer chemotherapy

Betulinic acid does not modulate the activity of P-gp/ABCB1 or MRP1/ABCC1 in a non-tumoral renal cell line: Possible utility in multidrug resistance cancer chemotherapy

Enrichment of pomace olive oil in triterpenic acids during storage of “Alpeorujo” olive paste

Oxidation of DNA Bases Influenced by the Presence of Other Bases

Contact Info

Product

Resources

About