Oleanolicacid-Chitosan Nanocomplex Induced Apoptotic Cell Death Through Mitochondrial Dysfunction in Human Lung Carcinoma: An Improved Synergetic Drug System for Cancer Therapy

Abulaiti, Abulimiti; Sun, Wei; Salai, Adili; Sun, Xiaohong; Yibulayin, Waresijiang; Gao, Yunfei

doi:10.1007/s10876-020-01934-0

Cited by 4 publications

(2 citation statements)

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“…This means that the values regarding the cytotoxic effect obtained for the SLN samples tested in the present study (cell viability rate of 28.38% for the SLN_OB 25@OA sample and 18.24% for the SLN_OB 80@OA sample) were due to the synergistic effect of the entire lipidic matrix. This statement is supported by a recent study carried out by Abulaiti and co-workers [99], in which the authors embedded oleanolic acid in a chitosan nano-complex and tested the anticancer potential of the conjugated nanocomplex (OAC) on A549 human lung cancer cells. The authors' results showed that the oleanolic acid entrapped in the chitosan polymeric matrix changes the cells' morphology, restrains cell development, and prompts apoptosis in A-549 cells, the cell apoptosis being predominantly accompanied by nuclear material fragmentation in the treated A549 cells.…”

Section: Discussionmentioning

confidence: 64%

“…The authors' results showed that the oleanolic acid entrapped in the chitosan polymeric matrix changes the cells' morphology, restrains cell development, and prompts apoptosis in A-549 cells, the cell apoptosis being predominantly accompanied by nuclear material fragmentation in the treated A549 cells. Thus, the authors portrayed the anticancer efficacy of oleanolic acid-conjugated chitosan nanoparticles as an improved synergistic drug system for cancer therapy [99].…”

Section: Discussionmentioning

confidence: 99%

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A Preliminary Report Regarding the Morphological Changes of Nano-Enabled Pharmaceutical Formulation on Human Lung Carcinoma Monolayer and 3D Bronchial Microtissue

Prodan-Bărbulescu,

Watz,

Moacă

et al. 2024

Medicina

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Background and Objectives: Nowadays, the development of enabled pharmaceutical nanoparticles of solid lipid type is continuously growing, because they have the potential to be used for targeted drug release leading to an increased effect of chemotherapy, being used in lung cancer nano-diagnosis and nano-therapy. The current study reports the preliminary results obtained regarding the biological effect of a new nano-enabled pharmaceutical formulation in terms of its cytotoxic and biosafety profile. Materials and Methods: The pharmaceutical formulations consist of solid lipid nanoparticles (SLN) obtained via the emulsification–diffusion method by loading green iron oxide nanoparticles (green-IONPs) with a pentacyclic triterpene (oleanolic acid—OA). Further, a complex biological assessment was performed, employing three-dimensional (3D) bronchial microtissues (EpiAirwayTM) to determine the biosafety profile of the SLN samples. The cytotoxic potential of the samples was evaluated on human lung carcinoma, using an in vitro model (A549 human lung carcinoma monolayer). Results: The data revealed that the A549 cell line was strongly affected after treatment with SLN samples, especially those that contained OA-loaded green-IONPs obtained with Ocimum basilicum extract (under 30% viability rates). The biosafety profile investigation of the 3D normal in vitro bronchial model showed that all the SLN samples negatively affected the viability of the bronchial microtissues (below 50%). As regards the morphological changes, all the samples induce major changes such as loss of the surface epithelium integrity, loss of epithelial junctions, loss of cilia, hyperkeratosis, and cell death caused by apoptosis. Conclusions: In summary, the culprit for the negative impact on viability and morphology of 3D normal bronchial microtissues could be the too-high dose (500 µg/mL) of the SLN sample used. Nevertheless, further adjustments in the SLN synthesis process and another complex in vitro evaluation will be considered for future research.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%