High infestations by the grapevine aphid Aphis illinoisensis (Hemiptera: Aphididae) have been observed in vineyards in southern Brazil, retarding plant growth and causing premature berry drop. This study evaluated the effect of insecticides on the control of the species in two experiments carried out in a greenhouse. The first control experiment for A. illinoisensis was conducted with seedlings of Vitis vinifera var. 'Cabernet Franc' to assess the effect of azadirachtin (Azamax ®) at dosages of 2.4 and 3.6 mL a.i. (active ingredient)/100 L of water, with reapplication seven days after the first application (DAFA). To compare its effect, the neonicotinoids imidacloprid (Provado 200 SC ®) and thiamethoxam (Actara 250 WG ®) were sprayed at dosages of 8 mL or g a.i./100 L of water in foliar application without reapplication. The second experiment compared the effect on A. illinoisensis by spraying these neonicotinoids at dosages of 8 mL or g a.i./100 L in foliar applications and of 0.05 mL or g a.i./100 L applied in the soil. Evaluations were performed at 0, 1, 5, 7, 10 and 14 DAFA. Imidacloprid and thiamethoxam effectively controlled A. illinoisensis in both forms of application [soil and foliar], while azadirachtin at the dosage of 3.6 ml a.i./100 L reapplied seven days after the first application provided 55.7% control. In conclusion, A. illinoisensis can be controlled effectively by employing neonicotinoids in the soil, while azadirachtin can be an alternative to reduce infestation pressure.