Olfaction-Related Gene Expression in the Antennae of Female Mosquitoes From Common Aedes aegypti Laboratory Strains

Mitra, Soumi; Pinch, Matthew; Kandel, Yashoda; Li, Yiyi; Rodriguez, Stacy D.; Hansen, Immo A.

doi:10.3389/fphys.2021.668236

Cited by 11 publications

(11 citation statements)

References 74 publications

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“…Interestingly, another study found differences in antennal olfactory gene expression across strains of Ae. aegypti , including members of the IR75 subfamily (Mitra et al . 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, another study found differences in antennal olfactory gene expression across strains of Ae. aegypti , including members of the IR75 subfamily (Mitra et al 2021). While the evidence is circumstantial, results like these may hint at differences in CA responses in adult females, depending on natural variations in populations or physiological states.…”

Section: Discussionmentioning

confidence: 99%

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Carboxylic acids that drive mosquito attraction to humans activate Ionotropic Receptors

Ray

Huff

Castillo

et al. 2022

Preprint

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The mosquito,Aedes aegypti, is highly anthropophilic and continues to transmit debilitating arboviruses within human populations. Female mosquitoes are attracted to sources of blood by responding to odor plumes that are emitted by their preferred hosts. Acidic volatile compounds, including carboxylic acids, represent particularly salient odors driving this attraction. Importantly, carboxylic acids are major constituents of human sweat and volatiles generated by skin microbes. As such, they are likely to impact human host preference, which is a dominant factor in disease transmission cycles. A more complete understanding of mosquito host attraction will necessitate the elucidation of molecular mechanisms of volatile odor detection that function in peripheral sensory neurons. Recent studies have shown that members of the variant ionotropic glutamate receptor gene family are necessary for physiological and behavioral responses to acidic volatiles inAedes. In this study, we have identified a subfamily of variant ionotropic receptors that share sequence homology across several important vector species and are likely to be activated by carboxylic acids. Moreover, we demonstrate that selected members of this subfamily are activated by short chain carboxylic acids in a heterologous cell expression system. Our results are consistent with the hypothesis that members of this receptor class underlie acidic volatile sensitivity in vector mosquitoes and provide a frame of reference for future development of novel mosquito attractant and repellent technologies.

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“…Interestingly, another study found differences in antennal olfactory gene expression across strains of Ae. aegypti , including members of the IR75 subfamily (Mitra et al . 2021).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Carboxylic acids that drive mosquito attraction to humans activate Ionotropic Receptors

Ray

Huff

Castillo

et al. 2022

Preprint

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“…Primers for nkaβ2 (Gene ID: 5573862) and ribosomal protein s7 ( rps7 ) were designed using PrimerBLAST [ 39 ] and NetPrimer (Premier Biosoft, Palo Alto, CA, USA) as previously described [ 40 ] (see Table 1 for primer sequences).…”

Section: Methodsmentioning

confidence: 99%

Knockdown of the Sodium/Potassium ATPase Subunit Beta 2 Reduces Egg Production in the Dengue Vector, Aedes aegypti

Martinez

Pinch

Kandel

et al. 2023

Insects

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The Na+/K+ ATPase (NKA) is present in the cellular membrane of most eukaryotic cells. It utilizes energy released by ATP hydrolysis to pump sodium ions out of the cell and potassium ions into the cell, which establishes and controls ion gradients. Functional NKA pumps consist of three subunits, alpha, beta, and FXYD. The alpha subunit serves as the catalytic subunit while the beta and FXYD subunits regulate the proper folding and localization, and ion affinity of the alpha subunit, respectively. Here we demonstrate that knockdown of NKA beta subunit 2 mRNA (nkaβ2) reduces fecundity in female Ae. aegypti. We determined the expression pattern of nkaβ2 in several adult mosquito organs using qRT-PCR. We performed RNAi-mediated knockdown of nkaβ2 and assayed for lethality, and effects on female fecundity. Tissue expression levels of nkaβ2 mRNA were highest in the ovaries with the fat body, midgut and thorax having similar expression levels, while Malpighian tubules had significantly lower expression. Survival curves recorded post dsRNA injection showed a non-significant decrease in survival of nkaβ2 dsRNA-injected mosquitoes compared to GFP dsRNA-injected mosquitoes. We observed a significant reduction in the number of eggs laid by nkaβ2 dsRNA-injected mosquitoes compared to control mosquitoes. These results, coupled with the tissue expression profile of nkaβ2, indicate that this subunit plays a role in normal female Ae. aegypti fecundity. Additional research needs to be conducted to determine the exact role played by NKAβ2 in mosquito post-blood meal nutrient sensing, transport, yolk precursor protein (YPP) synthesis and yolk deposition.

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“…Primers were designed to flank intron sequences to discriminate between mRNA and genomic DNA amplification products. Primers for the vitellogenin ( vg ), beta-actin ( β-actin ) and ribosomal protein S7 ( rps7 ) genes have been published previously [ 45 , 46 ] (see Table 3 for all primer sequences). All qRT-PCR primers were synthesized at the 10-nmol scale with standard desalting purification (Eurofins Genomics, Louisville, KY, USA).…”

Section: Methodsmentioning

confidence: 99%

General control nonderepressible 1 interacts with cationic amino acid transporter 1 and affects Aedes aegypti fecundity

et al. 2022

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Background The amino acid transporter protein cationic amino acid transporter 1 (CAT1) is part of the nutrient sensor in the fat body of mosquitoes. A member of the SLC7 family of cationic amino acid transporters, it is paramount for the detection of elevated amino acid levels in the mosquito hemolymph after a blood meal and the subsequent changes in gene expression in the fat body. Methods We performed a re-annotation of Aedes aegypti cationic amino acid transporters (CATs) and selected the C-terminal tail of CAT1 to perform a yeast two-hybrid screen to identify putative interactors of this protein. One interesting interacting protein we identified was general control nonderepressible 1 (GCN1). We determined the expression pattern of GCN1 in several adult organs and structures using qRT-PCR and western blots. Finally, we knocked down GCN1 using double-stranded RNA and identified changes in downstream signaling intermediates and the effects of knockdown on vitellogenesis and fecundity. Results In a screen for Ae. aegypti CAT1-interacting proteins we identified GCN1 as a putative interactor. GCN1 is highly expressed in the ovaries and fat body of the mosquito. We provide evidence that eukaryotic translation initiation factor 2 subunit alpha (eIF2α) phosphorylation changed during vitellogenesis and that RNA interference knockdown of GCN1 in whole mosquitoes reduced egg clutch sizes of treated mosquitoes relative to controls. Conclusions Aedes aegypti CAT1 and GCN1 are likely interacting partners and GCN1 is likely necessary for proper egg development. Our data suggest that GCN1 is part of a nutrient sensor mechanism in various mosquito tissues involved in vitellogenesis. Graphical Abstract

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Olfaction-Related Gene Expression in the Antennae of Female Mosquitoes From Common Aedes aegypti Laboratory Strains

Cited by 11 publications

References 74 publications

Carboxylic acids that drive mosquito attraction to humans activate Ionotropic Receptors

Carboxylic acids that drive mosquito attraction to humans activate Ionotropic Receptors

Knockdown of the Sodium/Potassium ATPase Subunit Beta 2 Reduces Egg Production in the Dengue Vector, Aedes aegypti

General control nonderepressible 1 interacts with cationic amino acid transporter 1 and affects Aedes aegypti fecundity

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