Olfactory ecto-mesenchymal stem cells possess immunoregulatory function and suppress autoimmune arthritis

Rui, Ke; Zhang, Zhijiang; Tian, Jie; Lin, Xiang; Wang, Xiaohui; Ma, Jie; Tang, Xinyi; Xu, Huaxi; Lu, Liwei; Wang, Shengjun

doi:10.1038/cmi.2015.82

Cited by 50 publications

(66 citation statements)

References 41 publications

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“…Phenotypic analysis reveals the presence of Stro-1 [75][76][77], CD29 [80,88], CD44 [80,88], CD49b [90], CD54 [13,77,82], CD73 [77,82,90], CD90 [13, 75-78, 80, 82, 88, 90], CD105 [13, 75-78, 80, 82], CD106 [77,78] and CD166 [77,78,80,82,90] markers and the absence of haematopoietic stem cell markers such as CD34 [13, 75-78, 80, 88, 90], CD45 [13, 75-78, 80, 88] and CD11b [88]. Strangely, one work indicates lack of Stro-1 [82].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

“…Then, they are cultured in lasks containing appropriate medium like Dulbecco's modiied Eagle's medium or HAM´s F-12 supplemented with fetal calf serum or fetal bovine serum, antibiotics, antifungals and growth factors [75]. After removal of non-adherent cells, the remaining cells can be trypsinized, expanded [82,88] and then banked in liquid nitrogen or induced to form for differentiation. [89].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

“…[89]. Multilineage diferentiation can be achieved by culturing under induction conditions and staining with speciic dyes [88].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

“…The described collection methods include a delicate discarding of the turbinates [82,88] or complete collection of the olfactory bulb [80], being the harvested material taken in balanced solutions containing antibiotics and antifungals [82]. The olfactory epithelium or olfactory bulb tissue sample is then cut into small pieces, digested with collagenase and DNase [80,82] or dispase [78] to separate the cells from their extracellular matrix.…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

“…In addition, the identiication of speciic proteins of these cells can be performed using immunoluorescence microscopy [88].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

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Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Alvites¹,

Santos²,

ProençaVarejão³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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The peripheral nerve injury after trauma is a common occurrence in both human and veterinary medicine and has severe consequences for the survival and quality of life of the patients. Despite the continuous eforts and the creation of diverse medical and surgical techniques, the harmful efects of this type of injury are far from being overcome. Regenerative medicine has been growing in the scientiic milieu as a new therapeutic approach for diferent situations. Among the cell-based therapies explored, the mesenchymal stem cells are evidenced by their features, versatility and potential applications. The olfactory mucosa mesenchymal stem cells, components of the olfactory system and identiied in the lamina propria, were newly identiied and are still undergoing characterization, appearing as a new promise in the regenerative therapy of several tissues but with special emphasis on the nervous system in general and the peripheral nervous system in particular, for which they appear to have special regenerative aptitude.

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Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

“…[89]. Multilineage diferentiation can be achieved by culturing under induction conditions and staining with speciic dyes [88].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

“…In addition, the identiication of speciic proteins of these cells can be performed using immunoluorescence microscopy [88].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

See 3 more Smart Citations

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Alvites¹,

Santos²,

ProençaVarejão³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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The Nasal Cavity of the Rat and Mouse—Source of Mesenchymal Stem Cells for Treatment of Peripheral Nerve Injury

Alvites

Caseiro

Pedrosa

et al. 2018

The Anatomical Record

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The nasal cavity performs several crucial functions in mammals, including rodents, being involved in respiration, behavior, reproduction, and olfaction. Its anatomical structure is complex and divided into several regions, including the olfactory recess where the olfactory mucosa (OM) is located and where the capture and interaction with the environmental odorants occurs. Among the cells of this region are the OM mesenchymal stem cells (MSCs), whose location raises the possibility that these cells could be involved in the peculiar ability of the olfactory nerve to regenerate continuously throughout life, although this relationship has not yet been confirmed. These cells, like all MSCs, present functional characteristics that make them candidates in new therapies associated with regenerative medicine, namely to promote the regeneration of the peripheral nerve after injury. The availability of stem cells to be therapeutically applied essentially depends on their collection in the tissue of origin. In the case of mice and rat's OM-MSCs, knowledge about the anatomy and histology of their nasal cavity is essential in establishing effective collection protocols. The present article describes the morphological characteristics of rodent's OM and establishes an alternative protocol for access to the olfactory recess and collection of the OM. Anat Rec, 301:1678-1689, 2018. © 2018 Wiley Periodicals, Inc.

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A TGF‐α and TGF‐β1 Poloxamer‐based micelle/hydrogel composite: A promising novel candidate for the treatment of anosmia

Moradi

Alizadeh

Yazdian

et al. 2022

Biotechnology Progress

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Anosmia is the inability to smell or loss of the sense of smell. It can reduce your ability to detect the smell of smoke, gas leaks, or spoiled food, as well as hinder the quality of life related to social interactions and feelings of well‐being. In the current study, a drug delivery composite was designed to cure anosmia and its efficiency in delivering transforming growth factor alpha (TGF‐α) and transforming growth factor beta 1 (TGF‐β1) to the nasal cavity was evaluated. Bovine serum albumin (BSA) was used as a model protein for encapsulation into Poloxamers 407 micelles. For the optimization of the BSA‐micelle formulation, a two‐parameter five‐level central composite design (CCD) was applied. The BSA‐micelle was optimized with a particle size of 41 nm, drug loading of 8%, and encapsulation efficiency of 74%. Further, the BSA‐micelle was characterized by FESEM, TEM, and FTIR. The analysis of release profile suggested high‐paced free BSA release compared to the gradual and prolonged release of BSA‐micelle/hydrogel and BSA‐micelles. The cytotoxicity assay demonstrated the safety of TGF‐α and TGF‐β1‐micelles/hydrogel. Moreover, it was observed that TGF‐α and TGF‐β1 within the hydrogels promote cellular viability and human olfactory ectomesenchymal stem cell OE‐MSCs proliferation. In conclusion, According to the results of our study, the TGF‐α and TGF‐β1‐micelle/hydrogel‐based delivery system provides a suitable alternative for anosmia treatment.

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Olfactory ecto-mesenchymal stem cells possess immunoregulatory function and suppress autoimmune arthritis

Cited by 50 publications

References 41 publications

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

The Nasal Cavity of the Rat and Mouse—Source of Mesenchymal Stem Cells for Treatment of Peripheral Nerve Injury

A TGF‐α and TGF‐β1 Poloxamer‐based micelle/hydrogel composite: A promising novel candidate for the treatment of anosmia

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