Olfactory signaling components and olfactory receptors are expressed in tubule cells of the human kidney

Kalbe, Benjamin; Schlimm, Marian; Wojcik, Sebastian; Philippou, Stathis; Maßberg, Désirée; Jansen, Fabian; Scholz, Paul; Luebbert, H; Ubrig, Burkhard; Osterloh, Sabrina; Hatt, Hanns

doi:10.1016/j.abb.2016.09.017

Cited by 35 publications

(35 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of OR51E2 beyond the cytoplasmic membrane was previously demonstrated in human prostate tissue and in endosomal organelles of melanocytes (Gelis et al, 2016 ; Massberg et al, 2016 ). The intracellular localization of further ORs was also described for OR2A4, which localized to the cytokinetic structures of HeLa cells and for other ORs in sperm, ratina, heart, kidney, and brain (Zhang et al, 2012 ; Garcia-Esparcia et al, 2013 ; Kalbe et al, 2016c ; Jovancevic et al, 2017a , b ). Moreover, the intracellular localization and function of GPCRs in RPE cells was previously shown for the well-characterized GPCR ocular albinism type 1 (OA1), which is predominantly localized at the membranes of melanosomes, the organelles of pigment synthesis, and late endosomes/lysosomes.…”

Section: Discussionmentioning

confidence: 85%

Odorant Receptor 51E2 Agonist β-ionone Regulates RPE Cell Migration and Proliferation

et al. 2017

Self Cite

View full text Add to dashboard Cite

The odorant receptor 51E2 (OR51E2), which is well-characterized in prostate cancer cells and epidermal pigment cells, was identified for the first time as the most highly expressed OR in human fetal and adult retinal pigment epithelial (RPE) cells. Immunofluorescence staining and Western blot analysis revealed OR51E2 localization throughout the cytosol and in the plasma membrane. Additionally, immunohistochemical staining of diverse layers of the eye showed that the expression of OR51E2 is restricted to the pigment cells of the RPE and choroid. The results of Ca2+-imaging experiments demonstrate that activation of OR51E2 triggers a Ca2+ dependent signal pathway in RPE cells. Downstream signaling of OR51E2 involves the activation of adenylyl cyclase, ERK1/2 and AKT. The activity of these protein kinases likely accounts for the demonstrated increase in the migration and proliferation of RPE cells upon stimulation with the OR51E2 ligand β-ionone. These findings suggest that OR51E2 is involved in the regulation of RPE cell growth. Thus, OR51E2 represents a potential target for the treatment of proliferative disorders.

show abstract

Section: Discussionmentioning

confidence: 85%

Odorant Receptor 51E2 Agonist β-ionone Regulates RPE Cell Migration and Proliferation

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…A recent paradigm in sensory physiology suggests that several classes of understudied receptors (olfactory receptors (ORs), taste receptors, and orphan GPRs) play key roles in nonsensory tissues, where they serve as selective and sensitive chemoreceptors [17,18]. Previous research showed that the intestinal STRs, T1R2 and T1R3, were expressed in distinct epithelial cells in the human proximal intestine and that their transcript levels varied with glycemic status in patients with type 2 diabetes [11,19].…”

Section: Discussionmentioning

confidence: 99%

Sweet-Taste Receptors Mediated ROS-NLRP3 Inflammasome Signaling Activation—Implications for Diabetic Nephropathy

Zhou¹,

Huang²,

Xu³

et al. 2018

Diabetes

View full text Add to dashboard Cite

Previous studies demonstrated that ROS-NLRP3 inflammasome signaling activation was involved in the pathogenesis of diabetic nephropathy (DN). Recent research has shown that sweet taste receptors (STRs) are important sentinels of innate immunity. Whether high glucose primes ROS-NLRP3 inflammasome signaling via STRs is unclear. In this study, diabetic mouse model was induced by streptozotocin (STZ) in vivo; mouse glomerular mesangial cells (GMCs) and human proximal tubular cells were stimulated by high glucose (10, 20, and 30 mmol/L) in vitro; STR inhibitor lactisole was used as an intervention reagent to evaluate the role and mechanism of the STRs in the pathogenesis of DN. Our results showed that the expression of STRs and associated signaling components (Gα-gustducin, PLCβ2, and TRPM5) was obviously downregulated under the condition of diabetes in vivo and in vitro. Furthermore, lactisole significantly mitigated the production of intracellular ROS and reversed the high glucose-induced decrease of Ca 2+ and the activation of NLRP3 inflammasome signaling in vitro (p < 0 05). These combined results support the hypothesis that STRs could be involved in the activation of ROS-NLRP3 inflammasome signaling in the pathogenesis of DN, suggesting that STRs may act as new therapeutic targets of DN.

show abstract

“…A number of other ORs (and taste receptors) have been identified in murine kidney (Rajkumar et al, 2014 ). ORs, along with G olf and AC3, have also been found in the human proximal tubule cell line HK-2 (Kalbe et al, 2016b ).…”

Section: Olfactory Receptorsmentioning

confidence: 97%

“…To date, four reports have focused on enterochromaffin (EC) cells. One showed that OR2J3 is expressed in a pancreatic EC cell line, and that helional (an OR2J3 ligand) acts to alter calcium signaling and serotonin release (Kalbe et al, 2016b ). Another paper identified four different ORs in microdissected human EC cells as well as in an EC cell line.…”

Section: Olfactory Receptorsmentioning

confidence: 99%

Olfactory, Taste, and Photo Sensory Receptors in Non-sensory Organs: It Just Makes Sense

et al. 2018

View full text Add to dashboard Cite

Sensory receptors that detect and respond to light, taste, and smell primarily belong to the G-protein-coupled receptor (GPCR) superfamily. In addition to their established roles in the nose, tongue, and eyes, these sensory GPCRs have been found in many ‘non-sensory' organs where they respond to different physicochemical stimuli, initiating signaling cascades in these extrasensory systems. For example, taste receptors in the airway, and photoreceptors in vascular smooth muscle cells, both cause smooth muscle relaxation when activated. In addition, olfactory receptors are present within the vascular system, where they play roles in angiogenesis as well as in modulating vascular tone. By better understanding the physiological and pathophysiological roles of sensory receptors in non-sensory organs, novel therapeutic agents can be developed targeting these receptors, ultimately leading to treatments for pathological conditions and potential cures for various disease states.

show abstract

Olfactory signaling components and olfactory receptors are expressed in tubule cells of the human kidney

Cited by 35 publications

References 37 publications

Odorant Receptor 51E2 Agonist β-ionone Regulates RPE Cell Migration and Proliferation

Odorant Receptor 51E2 Agonist β-ionone Regulates RPE Cell Migration and Proliferation

Sweet-Taste Receptors Mediated ROS-NLRP3 Inflammasome Signaling Activation—Implications for Diabetic Nephropathy

Olfactory, Taste, and Photo Sensory Receptors in Non-sensory Organs: It Just Makes Sense

Contact Info

Product

Resources

About