Oligodendrocyte ARNT2 expression is altered in models of MS

Becquart, Pierre; Johnston, Jake; Vilariño‐Güell, Carles; Quandt, Jacqueline A.

doi:10.1212/nxi.0000000000000745

Cited by 3 publications

(2 citation statements)

References 56 publications

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“…Arnt2 expression decreased with the maturation of the OPC cell line Oli-neu. Arnt2 knockdown increased the number of myelinating oligodendrocytes [85].…”

Section: Arnt2mentioning

confidence: 96%

Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93

et al. 2022

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Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS). Therapy is currently limited to drugs that interfere with the immune system; treatment options that primarily mediate neuroprotection and prevent neurodegeneration are not available. Here, we studied the effects of nimodipine on the rat cell line OLN-93, which resembles young mature oligodendrocytes. Nimodipine is a dihydropyridine that blocks the voltage-gated L-type calcium channel family members Cav1.2 and Cav1.3. Our data show that the treatment of OLN-93 cells with nimodipine induced the upregulation of myelin genes, in particular of proteolipid protein 1 (Plp1), which was confirmed by a significantly greater expression of PLP1 in immunofluorescence analysis and the presence of myelin structures in the cytoplasm at the ultrastructural level. Whole-genome RNA sequencing additionally revealed the upregulation of genes that are involved in neuroprotection, remyelination, and antioxidation pathways. Interestingly, the observed effects were independent of Cav1.2 and Cav1.3 because OLN-93 cells do not express these channels, and there was no measurable response pattern in patch-clamp analysis. Taking into consideration previous studies that demonstrated a beneficial effect of nimodipine on microglia, our data support the notion that nimodipine is an interesting drug candidate for the treatment of MS and other demyelinating diseases.

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“…Arnt2 expression decreased with the maturation of the OPC cell line Oli-neu. Arnt2 knockdown increased the number of myelinating oligodendrocytes [85].…”

Section: Arnt2mentioning

confidence: 96%

Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93

et al. 2022

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“…OLs are also heterogeneous under pathological conditions. OLs have long been considered as a natural target of MS disease, and studies have shown that human MS and its mouse model EAE have a high degree of OLs diversity (Becquart et al, 2020). Chang et al examined successive white cortical lesions in MS and found that cortical myelin regeneration exceeded WM myelin regeneration in lesions of the same age.…”

Section: The Heterogeneity Of Ols In Physiology and Pathologymentioning

confidence: 99%

Oligodendrocyte lineage cells: Advances in development, disease, and heterogeneity

Wang

Gao

et al. 2022

Journal of Neurochemistry

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The oligodendrocyte lineage cells include oligodendrocyte progenitor cells (OPCs) and oligodendrocytes (OLs). OPCs are the precursor cells of OLs in the central nervous system (CNS). During development, OPCs terminally differentiate and mature into myelinated OLs (Guo et al., 2018;Micu et al., 2018). OPCs are distributed throughout the CNS and are characterized by the high expression of PDGFRα and NG2 on such cells (Dawson et al., 2003;Sim et al., 2011). The primary function of OPCs is to differentiate into mature myelin-producing

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Genetic and neuro-epigenetic effects of divergent artificial selection for feather pecking behaviour in chickens

de Haas,

Pértille,

Kjaer

et al. 2024

BMC Genomics

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Feather pecking (FP) is a repetitive behaviour in chickens, influenced by genetic, epigenetic, and environmental factors, similar to behaviours seen in human developmental disorders (e.g., hyperactivity, autism). This study examines genetic and neuro-epigenetic factors in the thalamus of chickens from lines selected for seven generations for high or low FP behaviour (HFP or LFP). We integrate data on Differentially Methylated Regions (DMRs), Single Nucleotide Polymorphisms (SNPs), and Copy Number Variations (CNVs) in this controlled artificial selection process. Significant differences in behaviour, immunology, and neurology have been reported in these lines. We identified 710 SNPs in these lines that indicate new potentially important genes for FP such as TMPRSS6 (implicated in autism), and SST and ARNT2 (somatostatin function). CNV were the omic level most affected during selection. The largest CNVs found were in RIC3 (gain in HFP) and SH3RF2 (gain in LFP) genes, linked to nicotinic acetylcholine receptor regulation and human oncogenesis, respectively. Our study also suggests that promoters and introns are hotspots for CpG depletion. The overlapping of the omic levels investigated here with data from a public FP Quantitative Trait Loci (QTL) database revealed novel candidate genes for understanding repetitive behaviours, such as RTKN2, associated with Alzheimer’s disease in humans. This study suggests CNVs as a crucial initial step for genomic diversification, potentially more impactful than SNPs.

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Oligodendrocyte ARNT2 expression is altered in models of MS

Cited by 3 publications

References 56 publications

Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93

Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93

Oligodendrocyte lineage cells: Advances in development, disease, and heterogeneity

Genetic and neuro-epigenetic effects of divergent artificial selection for feather pecking behaviour in chickens

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