Oligodendrocyte cell surface recognized by a novel monoclonal antibody specific to sulfatide

Phenotype of arylsulfatase A-deficient mice: Relationship to human metachromatic leukodystrophy

“…Sections were incubated with monoclonal antibodies against glial fibrillary acidic protein to visualize astrocytes, (Sigma), F4͞80 for murine microglia (American Type Culture Collection), and sulfatide (provided by S. Ghandour, Laboratoire de Neurobiologie Ontogemque, Strasbourg, France) (17). The brain isoform NO synthase polyclonal antibody was from Biomol (Plymouth Meeting, PA).…”

Oligodendrocyte maturation and progenitor cell proliferation are independently regulated by thyroid hormone

“…After 12 h, 85 to 90% of the cells were stained with antibody to OL1, an early surface marker specific for early immature oligodendrocytes (Ghandour and Nussbaum, 1990). The remaining cells were either GFAP 1 , attesting to their astrocyte phenotype, or A2B5 1 , a phenotype of glial progenitors (O-2A).…”

“…As the progenitor differentiates into oligodendrocyte, it expresses successively a membrane sulfatide (OL1) (Ghandour and Nussbaum, 1990) and then galactocerebroside (GC), myelin basic protein (MBP), and myelin/oligodendrocyte glycoprotein (MOG) (Cameron and Rakic, 1991;Pfeiffer et al, 1993).…”

HPC-7: A novel oligodendrocyte lineage protein which appears prior to galactocerebroside

“…Polyclonal rabbit anti-GC and anti-GFAP antibodies (1/100 dilution) were purchased from Sigma (St Louis, MO). OL-1 rat mAb (1/200 dilution) was kindly provided by Dr. S. Ghandour (Ghandour and Nussbaum, 1990). Polyclonal rabbit anti-MBP (1/50 dilution) and anti-CGT (1/50 dilution) antibodies were a gift of Dr. Neskovic.…”

“…The O-2A progenitor cell is defined by the expression of the tetrasialoganglioside antigen A2B5 (Eisenbarth et al, 1979;Levi et al, 1987). An intermediate stage appears next, characterized by the successive appearance of an unidentified lipid and sulfatides recognized respectively by O4 and OL-1 antibodies Ghandour and Nussbaum, 1990;Sommer and Schachner, 1981). These distinguish one stage of multipolar OL progenitors called proligodendroblasts , and represent an important stage in development, heralding the imminent postmitotic differentiation into OL (Bansal et al, 1989Knapp, 1991;Sommer and Schachner, 1981).…”