Oligonucleotide microarray analysis of differential transporter regulation in the regenerating rat liver

Dransfeld, Olaf; Gehrmann, Thor; Köhrer, Karl; Kircheis, G.; Holneicher, Claudia; Häussinger, Dieter; Wettstein, Matthias

doi:10.1111/j.1478-3231.2005.01158.x

Cited by 43 publications

(36 citation statements)

References 55 publications

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“…Functionally, prolactin treatment of ovarectomized rats leads to an increase of ATP-dependent bile salt transport into canalicular vesicles without a change of the K m value [199]. After partial hepatectomy as a model for the regenerating liver, Bsep expression remains practically unchanged at the mRNA and at the protein level [331] or is mildly upregulated [64,93]. This is paralleled by an unchanged ATP-dependent taurocholate transport into isolated canalicular plasma membrane vesicles from regenerating rat liver [99].…”

Section: Pathophysiologymentioning

confidence: 85%

See 1 more Smart Citation

The Role of the Sodium-Taurocholate Cotransporting Polypeptide (NTCP) and of the Bile Salt Export Pump (BSEP) in Physiology and Pathophysiology of Bile Formation

Stieger

2010

Handbook of Experimental Pharmacology

246

260

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Section: Pathophysiologymentioning

confidence: 85%

“…After delivery, the expression of Ntcp is increased [36,84]. Finally, in the regenerating rat liver, Ntcp is transiently down regulated both at the mRNA, protein and functional level [64,93,99,331].…”

Section: Pathophysiologymentioning

confidence: 94%

The Role of the Sodium-Taurocholate Cotransporting Polypeptide (NTCP) and of the Bile Salt Export Pump (BSEP) in Physiology and Pathophysiology of Bile Formation

Stieger

2010

Handbook of Experimental Pharmacology

246

260

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“…Most recently, a growing body of evidence suggests that the beneficial effect of EPO treatment on liver resection and living donor liver transplantation in rats [14,[17][18]. Moreover, microarray analysis is a powerful approach for monitoring molecular events during liver regeneration after PH, which has been demonstrated in previous studies [24,25,[33][34][35][36][37][38][39][45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

“…However, HCs are the primary cell type responsible for Epo expression in the liver and the liver maintains the capacity to express EPO [45]. Rankin et al found that Hif-2 regulated hepatic EPO expression in the early postnatal, Vhlh-deficient, and anemic adult liver [46]. Most recently, a growing body of evidence suggests that the beneficial effect of EPO treatment on liver resection and living donor liver transplantation in rats [14,[17][18].…”

Section: Discussionmentioning

confidence: 99%

Expression profiles uncover the relationship between erythropoietin and cell proliferation in rat hepatocytes after a partial hepatectomy

Zhang

Yang

et al. 2014

Cellular and Molecular Biology Letters

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Erythropoietin (EPO) has a beneficial effect on hepatic cell proliferation during liver regeneration. However, the underlying mechanism has not yet been elucidated. To uncover the proliferation response of EPO in rat liver regeneration after partial hepatectomy (PH) at the cellular level, hepatocytes (HCs) were isolated using Percoll density gradient centrifugation. The genes of the EPO-mediated signaling pathway and the target genes of the transcription factor (TF) in the pathway were identified in a pathway and TF database search. Their expression profiles were then detected using Rat Genome 230 2.0 Microarray. The results indicated that the EPO-mediated signaling pathway is involved in 19 paths and that 124 genes participate, of which 32 showed significant changes and could be identified as liver regeneration-related genes. In addition, 443 targets regulated by the TFs of the pathway and 60 genes associated with cell proliferation were contained in the array. Subsequently, the synergetic effect of these genes in liver regeneration was analyzed using the E(t) mathematical model based on their expression profiles. The results demonstrated that the E(t) values of paths 3, 8, 12 and 14–17 were significantly strengthened in the progressing phase of liver regeneration through the RAS/MEK/ERK or PI3K/AκT pathways. The synergetic effect of the target genes, in parallel with target-related cell proliferation, was also enhanced 12–72 h after PH, suggesting a potential positive effect of EPO on HC proliferation during rat liver regeneration. These data imply that the EPO receptor may allow EPO to promote HC proliferation through paths 3, 8, 12 and 14–17, mediating the RAS/MEK/ERK and PI3K/AκT pathways in rat liver regeneration after PH.

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“…Four measurement time points were adopted; 0, 24, 72 and 168 h. There are some reports of microarray analyses related to liver regeneration, but most have been measured in the early phases of regeneration (26,(32)(33)(34)(35). Because our concern is more specific to the changes in the ABC transporters, we analyzed the later phase according to the data obtained in the previous report (20).…”

Section: Discussionmentioning

confidence: 99%

Gene expression of ATP-binding cassette transporters during liver regeneration after 90% hepatectomy in rats

et al. 2012

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Abstract. Liver damage with hyperbilirubinemia during regeneration of the small liver is the major hurdle to expand the indications of adult living donor liver transplantation. We performed a large-scale gene expression analysis of the regenerating liver after a 90% hepatectomy in rats, and analyzed the changes in the gene expression patterns related to the ATP-binding cassette (ABC) transporters. RNAs were prepared from 3 rat livers at 0, 24, 72 and 168 h after a 90% hepatectomy. The gene expression profile was analyzed by the Rat Genome 230 2.0 array with special references to the ABC transporters. Among 31,042 probes, 1,587 reported genes were identified as either upregulated or downregulated more than 2-fold. Among 20 ABC transporter genes, multidrug resistance protein (MRP) 2 and organic anion transporting polypeptide (OATP) 1 were significantly downregulated, while MRP1 and MRP3 tended to be expressed. These genetic changes were confirmed by real-time PCR. A microarray analysis demonstrated not only an extensive gene expression profile in the regenerating liver but more specific molecular events related to bilirubin transport at the same time. Changes in the expression pattern of the ABC transporters, therefore, seem to be the key event in liver failure during liver regeneration.

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Oligonucleotide microarray analysis of differential transporter regulation in the regenerating rat liver

Abstract: The data show a differential regulation of hepatic transport systems during liver regeneration.

Cited by 43 publications

References 55 publications

The Role of the Sodium-Taurocholate Cotransporting Polypeptide (NTCP) and of the Bile Salt Export Pump (BSEP) in Physiology and Pathophysiology of Bile Formation

The Role of the Sodium-Taurocholate Cotransporting Polypeptide (NTCP) and of the Bile Salt Export Pump (BSEP) in Physiology and Pathophysiology of Bile Formation

Expression profiles uncover the relationship between erythropoietin and cell proliferation in rat hepatocytes after a partial hepatectomy

Gene expression of ATP-binding cassette transporters during liver regeneration after 90% hepatectomy in rats

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