Oligonucleotide Probes for the Specific Detection of the Wild Poliovirus Types 1 and 3 Endemic to Brazil

Silva, Edson E. da; Pallansch, Mark A.; Holloway, Brian P.; Oliveira, Maria José Couto; Schatzmayr, Hermann G.; Kew, Olen M.

doi:10.1159/000150195

Cited by 32 publications

(28 citation statements)

References 27 publications

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“…Direct identifi cation of wild polioviruses was only possible with wild genotype-specifi c probes [ 4 ] and PCR primer sets [ 7 ]. At that time, the current catalog of wild-genotype-specifi c molecular Viral RNA Non-infectious control RNA reagents did not cover all of the many different poliovirus genotypes still in circulation worldwide [ 9 ].…”

Section: Early Poliovirus Diagnosticsmentioning

confidence: 99%

“…4 , Table 1 ). These VDPV assays have several advantages including (1) sharply reducing the workload to sequence vaccine-related isolates to screen for cVDPVs ; (2) being more sensitive (at least for detecting S2 VDPVs) than the ELISA assay in detecting early genetic changes associated with VDPV emergence [ 34 ] and (3) 4 Amplifi cation of reference Sabin strain (S, serotype indicated by number) sequences in the rRT-PCR VDPV assays. Assays used the FAM fl uorophore for targeting the VP1 gene.…”

Section: Vdpv Dual-stage Assaymentioning

confidence: 99%

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Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing by PCR and Real-Time RT-PCR

Burns¹,

Kilpatrick²,

Iber³

et al. 2016

Methods in Molecular Biology

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Virologic surveillance is essential to the success of the World Health Organization initiative to eradicate poliomyelitis. Molecular methods have been used to detect polioviruses in tissue culture isolates derived from stool samples obtained through surveillance for acute flaccid paralysis. This chapter describes the use of realtime PCR assays to identify and serotype polioviruses. In particular, a degenerate, inosine-containing, panpoliovirus (panPV) PCR primer set is used to distinguish polioviruses from NPEVs. The high degree of nucleotide sequence diversity among polioviruses presents a challenge to the systematic design of nucleic acid-based reagents. To accommodate the wide variability and rapid evolution of poliovirus genomes, degenerate codon positions on the template were matched to mixed-base or deoxyinosine residues on both the primers and the TaqMan™ probes. Additional assays distinguish between Sabin vaccine strains and non-Sabin strains. This chapter also describes the use of generic poliovirus specific primers, along with degenerate and inosine-containing primers, for routine VP1 sequencing of poliovirus isolates. These primers, along with nondegenerate serotype-specific Sabin primers, can also be used to sequence individual polioviruses in mixtures.

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Section: Early Poliovirus Diagnosticsmentioning

confidence: 99%

Section: Vdpv Dual-stage Assaymentioning

confidence: 99%

Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing by PCR and Real-Time RT-PCR

Burns¹,

Kilpatrick²,

Iber³

et al. 2016

Methods in Molecular Biology

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“…The antigenic method was an ELISA with cross-absorbed intratype-specific rabbit antisera [3,5,6]. The genetic method was a hybridisation assay with recombinant riboprobes which specifically hybridise to the genome of vaccine-related isolates [ 13,181. The combination of these two methods was recently shown to be the most appropriate and recommended for use in the WHO polio laboratory network [30].…”

Section: Intratypic Differentiation Of Poliovirusesmentioning

confidence: 99%

Molecular epidemiology of poliovirus infection in Tunisia

Triki

Bahri

Guillot

et al. 1999

Journal of Medical Microbiology

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This report is an overview of poliomyelitis surveillance in Tunisia from 1991 to 1996. In all, 2088 stool specimens, collected from 152 acute flaccid paralysis (AFP) cases and from 1747 of their healthy contacts were investigated. Virus isolation was done systematically in RD and HEp-2C cell lines and isolated viruses were typed by seroneutralisation as polioviruses or non-polio enteroviruses. Poliovirus isolates were analysed systematically for their wild or vaccine-related origin by two methods -one based on antigenic differences and one on genetic differences between strains. All type 2 polioviruses were vaccine-related and most wild viruses belonged to polio serotype 3. Wild polio type 3 viruses were detected in 1991 and 1992 in six cases of paralytic polio. A silent circulation of wild polio 1 and wild polio 3 was detected in 1994. No wild virus was detected in Tunisia from 1995 onwards. Wild polioviruses were sequenced and compared with Tunisian wild strains isolated during the 1980s, as well as other genotypes from the international database. These investigations revealed a single Tunisian polio 3 genotype that has been circulating from 1985 to 1994 and two different polio 1 genotypes. These results reflect effective control strategies within the country and contribute to the improvement of the polio eradication programme effectiveness at national and global levels.

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“…Based on the genomic sequencing of strains of wild polioviruses, different wild poliovirus strains apparently occupied distinct geographic areas, and these areas were steadily shrinking in size (6,34). Moreover, sustained transmission, as with smallpox, appeared to require crowded, lower socioeco nomic popUlations.…”

Section: Accelerated Strategies: the Final Stagesmentioning

confidence: 99%

Polio Eradication From the Western Hemisphere

et al. 1992

Annu. Rev. Public Health

114

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Oligonucleotide Probes for the Specific Detection of the Wild Poliovirus Types 1 and 3 Endemic to Brazil

Cited by 32 publications

References 27 publications

Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing by PCR and Real-Time RT-PCR

Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing by PCR and Real-Time RT-PCR

Molecular epidemiology of poliovirus infection in Tunisia

Polio Eradication From the Western Hemisphere

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