“Omics” Approaches for Understanding Soil Suppressiveness in Agriculture

Khatri, Shivani; Bhattacharjee, Annapurna; Sharma, Shilpi

doi:10.1007/978-981-16-0889-6_4

Cited by 4 publications

(1 citation statement)

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“…Soil is the foundation of agricultural production and the microorganisms therein are considered to be key determinants of crop growth and health. Disease‐suppressive soils are the hallmark of how soil microbes can benefit plant health in agroecosystems (Schroth & Hancock, 1982; Rosenzweig et al ., 2012; Gómez Expósito et al ., 2017; Topalović et al ., 2020; Khatri et al ., 2021). Among them, Pseudomonas is well‐known for its role in take‐all decline soils owing to 2,4‐diacetylphloroglucinol production (Shen et al ., 2015).…”

Section: Discussionmentioning

confidence: 99%

Deciphering the mechanism of fungal pathogen‐induced disease‐suppressive soil

et al. 2023

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One model of a disease-suppressive soil predicts that the confrontation of plant with a phytopathogen can lead to the recruitment and accumulation of beneficial microorganisms. However, more information needs to be deciphered regarding which beneficial microbes become enriched, and how the disease suppression is achieved.Here, we conditioned soil by continuously growing eight generations of cucumber inoculated with Fusarium oxysporum f.sp. cucumerinum in a split-root system.Disease incidence was found to decrease gradually upon pathogen infection accompanied with higher quantity of reactive oxygen species (ROS mainly OH • ) in roots and accumulation of Bacillus and Sphingomonas. These key microbes were proven to protect the cucumber from pathogen infection by inducing high ROS level in the roots through enrichment of pathways, including a two-component system, a bacterial secretion system, and flagellar assembly revealed by metagenomics sequencing. Untargeted metabolomics analysis combined with in vitro application assays suggested that threonic acid and lysine were pivotal to recruit Bacillus and Sphingomonas.Collectively, our study deciphered a 'cry for help' case, wherein cucumber releases particular compounds to enrich beneficial microbes that raise the ROS level of host to prevent pathogen attack. More importantly, this may be one of the fundamental mechanisms underpinning disease-suppressive soil formation.

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