Omnidirectional leaky opto-electrical fiber for optogenetic control of neurons in cell replacement therapy

Vasudevan, Shashank; Dotti, A.; Kajtez, Janko; Martínez‐Serrano, Alberto; Gundlach, Carsten; Maçãs, Sandrina Campos; Lauschke, Karin; Vinngaard, Anne-Marie; López, Silvia García; Pereira, Marta P.; Heiskanen, Arto; Keller, Stephan Sylvest; Emnéus, Jenny

doi:10.1016/j.bioelechem.2022.108306

Cited by 2 publications

(6 citation statements)

References 36 publications

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“…Since the goal of the Training4CRM project was to have a brain-implantable device, which features an electrode chip with much smaller WE area [ 34 , 35 ], our platform can easily provide the required optical power for such devices. The opto-electrical fiber will be integrated with our multisensor headstage platform in a future iteration to perform in vitro and in vivo experiments using the integrated optical stimulator.…”

Section: Discussionmentioning

confidence: 99%

“…The developed multisensor platform is designed to be interfaced with a specially developed leaky opto-electrical fiber [ 34 , 35 ]. This fiber is to be grafted with optogenetically modified neural progenitor cells.…”

Section: Methodsmentioning

confidence: 99%

“…To minimize study variables and to facilitate in vitro measurements, a previously studied in vitro cell culture and electrode system was used as SUT. The specially developed leaky opto-electrical fiber was studied separately using the same cell line [ 34 , 35 ]. The full integration of the leaky opto-electrical fiber with the electronic interface will be approached in future studies.…”

Section: Methodsmentioning

confidence: 99%

“…Exocytosis of neurotransmitters was either triggered by depolarizing the cell membrane by injecting a high K + buffer extracellularly to the cell culture well (10 mM HEPES, 5 mM glucose, 1.2 mM MgCl 2 , 2 mM CaCl 2 , 5 mM NaCl, and 450 mM KCl) or by optical stimulation of the optogenetically modified cells with blue light (450 nm). The electrode chips used in this study had a much larger working area than the leaky opto-electrical fiber [ 34 , 35 ] that was planned to be interfaced with the headstage platform. Specifications of the system were defined and a suitable laser diode was chosen based on the leaky opto-electrical fiber.…”

Section: Methodsmentioning

confidence: 99%

“…After the cells were differentiated into dopaminergic neurons, we stimulated them optically and performed amperometric measurements to monitor dopamine release. In future studies, we plan to interface this platform, which is designed to be an opto-electric headstage, with a cell grafted leaky opto-electrical fiber [ 34 , 35 ] that has been developed in parallel in the Training4CRM consortium (European Training Network for Cell-based Regenerative Medicine) to proceed to in vivo studies. The use of both impedance and electrochemical measurements provides a more comprehensive understanding of the performance of dopaminergic neurons and can aid in the development and optimization of cell-based therapies for PD and other dopamine-related disorders.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant

Cunha,

Schuelke,

Mesri

et al. 2024

Sensors

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Implantable cell replacement therapies promise to completely restore the function of neural structures, possibly changing how we currently perceive the onset of neurodegenerative diseases. One of the major clinical hurdles for the routine implementation of stem cell therapies is poor cell retention and survival, demanding the need to better understand these mechanisms while providing precise and scalable approaches to monitor these cell-based therapies in both pre-clinical and clinical scenarios. This poses significant multidisciplinary challenges regarding planning, defining the methodology and requirements, prototyping and different stages of testing. Aiming toward an optogenetic neural stem cell implant controlled by a smart wireless electronic frontend, we show how an iterative development methodology coupled with a modular design philosophy can mitigate some of these challenges. In this study, we present a miniaturized, wireless-controlled, modular multisensor platform with fully interfaced electronics featuring three different modules: an impedance analyzer, a potentiostat and an optical stimulator. We show the application of the platform for electrical impedance spectroscopy-based cell monitoring, optical stimulation to induce dopamine release from optogenetically modified neurons and a potentiostat for cyclic voltammetry and amperometric detection of dopamine release. The multisensor platform is designed to be used as an opto-electric headstage for future in vivo animal experiments.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant

Cunha,

Schuelke,

Mesri

et al. 2024

Sensors

Self Cite

View full text Add to dashboard Cite

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Vortex‐Beam Multiplexing Emitter Using Advanced Manufactured Leaky Cables

Deng,

Luk,

Sun

et al. 2024

Advanced Photonics Research

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Although orbital angular momentum (OAM) is extensively explored and researched in various scientific domains, its practical application in wireless communications still faces numerous challenges. Notably, existing OAM beam generation technologies need to meet the diversified demands of modern wireless communication, such as miniaturization, cost‐effectiveness, and system integration. Driven by the immense potential of OAM in communication systems, in this work, an innovative method based on leaky cables capable of simultaneously generating multiple vortex beams, overcoming a series of implementation issues associated with multiplexing emitters, is introduced. A design prototype is fabricated and tested, and the measurement results align closely with the simulations, validating the multifunctionality and practicality of the method. Moreover, the proposed strategy can seamlessly extend to optical frequencies using the concept of leaky fibers. In this method, not only efficient spatial utilization and excellent isolation are realized but also high cost‐effectiveness is possessed. Compared to previous strategies, this technique may have an impact on simplifying the establishment of communication system platforms based on OAM multiplexing.

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Omnidirectional leaky opto-electrical fiber for optogenetic control of neurons in cell replacement therapy

Cited by 2 publications

References 36 publications

Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant

Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant

Vortex‐Beam Multiplexing Emitter Using Advanced Manufactured Leaky Cables

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