OmpW expressed by recombinant Lactobacillus casei elicits protective immunity against Aeromonas veronii in common carp

Zhang, Lei; Liu, Zhenxing; Li, Ying; Tian, Jingkui; Jia, Kaixiang; Zhang, Dongxing; Song, Ming-Fang; Raza, Sayed Haidar Abbas; Garcia, Matthew; Kang, Yuan-Huan; Zheng, Wei Xing; Ai-dong, Qian; Xu, Yongping

doi:10.1016/j.micpath.2019.103552

Cited by 17 publications

(17 citation statements)

References 35 publications

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“…There, the immune cells could stimulate other cells either by endogenous release of interferon or by the release of interferon produced by MT006, in a mechanism similar to that observed when dendritic cells interact with commensal bacteria in the mammalian intestine and transport them to the lymph nodes (89). This mechanism could explain the immunizing properties of orally administered recombinant Lactic Acid Bacteria that express epitopes from microbial pathogens of fish (33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44). The dosages used in these experiments are between 10 7 to 2x10 8 CFU per fish gram, around 30 to 600 times the used dosage of MT006.…”

Section: Discussionmentioning

confidence: 91%

“…In salmonids, oral administration of Lactobacillus casei species expressing IPNV epitopes has shown to confer protection against the virus (33)(34)(35)(36)(37), while Lactococcus lactis strains have been used to orally immunize against Hirame novirhabdovirus in flounder (38), Carp spring viremia in common carps (39), the protozoa Ichthyophthirius multifiliis in Goldfish (40), and against the viral hemorrhagic septicemia in rainbow trout (41). Oral administration of recombinant Lactobacillus casei expressing epitopes of Aeromona veronii confers protection against this pathogen in Cyprinus carpio (42,43), and Carassius carassius (44), while Lactobacillus plantarum expressing G protein of spring viremia of carp virus (SVCV) (45) and the ORF81 protein of koi herpesvirus (KHV) has shown confers protection against both viruses with high titers of IgM after its oral administration to Cyprinus carpio (46). Altogether, these results have shown that lactic acid bacteria are an efficient vehicle for the release of immunostimulant peptides in fish.…”

Section: Introductionmentioning

confidence: 99%

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Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

et al. 2021

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In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids depends on the proper design of a vehicle that allows its massive administration, ideally orally. In mammals, administration of recombinant probiotics capable of expressing cytokines has shown local and systemic therapeutic effects. In this work, we evaluate the use of Lactococcus lactis as a type I Interferon expression system in Atlantic salmon, and we analyze its ability to stimulate the antiviral immune response against IPNV, in vivo and in vitro. The interferon expressed in L. lactis, even though it was located mainly in the bacterial cytoplasm, was functional, stimulating Mx and PKR expression in CHSE-214 cells, and reducing the IPNV viral load in SHK-1 cells. In vivo, the oral administration of this L. lactis producer of Interferon I increases Mx and PKR expression, mainly in the spleen, and to a lesser extent, in the head kidney. The oral administration of this strain also reduces the IPNV viral load in Atlantic salmon specimens challenged with this pathogen. Our results show that oral administration of L. lactis producing Interferon I induces systemic effects in Atlantic salmon, allowing to stimulate the antiviral immune response. This probiotic could have effects against a wide variety of viruses that infect Atlantic salmon and also be effective in other salmonids due to the high identity among their type I interferons.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

et al. 2021

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“…The literature accumulates evidence that Lactobacillus casei is also a prominent live vehicle for expressing and transporting heterologous antigens to mucosal sites. For example, the recombinant Lactobacillus casei can be detected in the digestive tract of common carp following oral administration, and colonization was shown to be higher in the hind-gut than in the prosogaster and mid-gut (158)(159)(160)(161). Meanwhile, these studies also showed that oral vaccination of common carp with Lactobacillus casei (1-2 × 10 9 CFU/g feed) displaying antigen(s) (surface-displayed or secreted) from Aeromonas veronii resulted in elevated transcript level of immune genes in the intestine postvaccination and provided strong protection for common carps against this pathogenic bacteria (158-161).…”

Section: Vaccines and Adjuvantsmentioning

confidence: 99%

Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

et al. 2021

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The gastrointestinal immune system plays an important role in immune homeostasis regulation. It regulates the symbiotic host-microbiome interactions by training and developing the host’s innate and adaptive immunity. This interaction plays a vital role in host defence mechanisms and at the same time, balancing the endogenous perturbations of the host immune homeostasis. The fish gastrointestinal immune system is armed with intricate diffused gut-associated lymphoid tissues (GALTs) that establish tolerance toward the enormous commensal gut microbiome while preserving immune responses against the intrusion of enteric pathogens. A comprehensive understanding of the intestinal immune system is a prerequisite for developing an oral vaccine and immunostimulants in aquaculture, particularly in cultured fish species. In this review, we outline the remarkable features of gut immunity and the essential components of gut-associated lymphoid tissue. The mechanistic principles underlying the antigen absorption and uptake through the intestinal epithelial, and the subsequent immune activation through a series of molecular events are reviewed. The emphasis is on the significance of gut immunity in oral administration of immunoprophylactics, and the different potential adjuvants that circumvent intestinal immune tolerance. Comprehension of the intestinal immune system is pivotal for developing effective fish vaccines that can be delivered orally, which is less labour-intensive and could improve fish health and facilitate disease management in the aquaculture industry.

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“…A . veronii mounting vaccine could effectively stimulate antibodies production against the pathogen and had good immune protection effect on carp (Song et al, 2018; Zhang et al, 2019). E .…”

Section: Introductionmentioning

confidence: 99%

A bivalent vaccine comprised of inactivated Aeromonas veronii and Edwardsiella ictaluri stimulates protective immune responses in yellow‐head catfish, Pelteobagrus fulvidraco

Zeng

Ouyang

Wang

et al. 2021

Aquaculture Research

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Aeromonas veronii and Edwardsiella ictaluri are two pathogenic bacteria in fish and could result in high mortality and considerable economic losses in aquaculture (Huang et al., 2019;Li et al., 2020).A. veronii is a Gram-negative, rod-shaped and facultative anaerobic bacterium and widely distributed in nature, with strong environmental adaptability (Parte, 2018). It was a severe causative agent of ulcer

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OmpW expressed by recombinant Lactobacillus casei elicits protective immunity against Aeromonas veronii in common carp

Cited by 17 publications

References 35 publications

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

Lactococcus lactis Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response In Vivo and In Vitro

Gut Immune System and the Implications of Oral-Administered Immunoprophylaxis in Finfish Aquaculture

A bivalent vaccine comprised of inactivated Aeromonas veronii and Edwardsiella ictaluri stimulates protective immune responses in yellow‐head catfish, Pelteobagrus fulvidraco

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