On-a-Chip-Based Sensitive Detection of Drug-Induced Apoptosis in Polarized Gastric Epithelial Cells

Bakhchova, Liubov; Jantaree, Phatcharida; Gupta, Anubhuti; Isermann, Berend; Steinmann, Ulrike; Naumann, Michael

doi:10.1021/acsbiomaterials.1c01094

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…It is highly hydrophobic, is transparent in the visible spectrum, is flexible and biocompatible. These characteristics made PDMS attractive for several biomedical applications and microfluidics [ 98 ].…”

Section: Methods For Manufacturing Microfluidic Structures For Biosen...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

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Proteins in biological fluids (blood, urine, cerebrospinal fluid) are important biomarkers of various pathological conditions. Protein biomarkers detection and quantification have been proven to be an indispensable diagnostic tool in clinical practice. There is a growing tendency towards using portable diagnostic biosensor devices for point-of-care (POC) analysis based on microfluidic technology as an alternative to conventional laboratory protein assays. In contrast to universally accepted analytical methods involving protein labeling, label-free approaches often allow the development of biosensors with minimal requirements for sample preparation by omitting expensive labelling reagents. The aim of the present work is to review the variety of physical label-free techniques of protein detection and characterization which are suitable for application in micro-fluidic structures and analyze the technological and material aspects of label-free biosensors that implement these methods. The most widely used optical and impedance spectroscopy techniques: absorption, fluorescence, surface plasmon resonance, Raman scattering, and interferometry, as well as new trends in photonics are reviewed. The challenges of materials selection, surfaces tailoring in microfluidic structures, and enhancement of the sensitivity and miniaturization of biosensor systems are discussed. The review provides an overview for current advances and future trends in microfluidics integrated technologies for label-free protein biomarkers detection and discusses existing challenges and a way towards novel solutions.

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Section: Methods For Manufacturing Microfluidic Structures For Biosen...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

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“…Biosensor and microfluidic technologies have demonstrated great potential in the field of medical testing and food quality control [25][26][27]. The development of biosensors for the express testing of fish freshness is particularly active, as low testing time and the possibility of on-site analysis play a crucial role here.…”

Section: K = [Ino] + [Hx] [Atp] + [Adp] + [Amp] + [Imp] + [Ino] + [Hx]mentioning

confidence: 99%

Fast Protein and Metabolites (Nucleotides and Nucleosides) Liquid Chromatography Technique and Chemical Sensor for the Assessment of Fish and Meat Freshness

Kuznetsov¹,

Frorip²,

Sünter

et al. 2023

Chemosensors

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Fast protein and metabolite liquid chromatography (FPLMC) was introduced years ago to enable the easy separation of high-molecular compounds such as proteins from small molecules and the identification of the low-molecular substances. In this paper, the method is applied for the rapid evaluation of freshness and monitoring the aging of animal meat and fish. A novel chromatographic sensor was developed with a deep UV LED-based photometric detection unit (255–265 nm), an original flow cuvette and registration scheme; the processing of a chromatogram with the sensor takes approximately 15 min. Strict isochronism between the elution of ATP metabolites, mainly hypoxanthine (Hx) and inosine monophosphate (IMP), and the time of maturation of meat or fish, was discovered. A new freshness index H* = [Hx]/[IMP] was introduced, which is proportional to the instrumental delay time in the FPMLC chromatograms: the H* index < 0.5 indicates the presence of inosine monophosphate (IMP) and the high quality of the meat or fish. Reasonably strong correlations were revealed between data obtained by FPMLC and total volatile basic nitrogen TVB-N (for fish) or volatile fatty acids VFA (for meat) content. Moreover, putative nucleotide salvage and an increase in the concentration of IMP were observed in fish after heat treatment using the FPMLC sensor and NMR technique.

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“…Signaling effectors of permeability are generally associated with calcium-dependent pathways (including PKC, Src family kinases, and RhoA) 301 , as well as the output metrics of polarity and alignment 302 . Shear forces have been also demonstrated to exert direct effects on function in models of the gastric epithelium 303,304 and liver 305 .…”

Section: Physical Signaling and Cell-cell Contact In Maturationmentioning

confidence: 99%

Harnessing conserved signaling and metabolic pathways to enhance the maturation of functional engineered tissues

et al. 2022

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The development of induced-pluripotent stem cell (iPSC)-derived cell types offers promise for basic science, drug testing, disease modeling, personalized medicine, and translatable cell therapies across many tissue types. However, in practice many iPSC-derived cells have presented as immature in physiological function, and despite efforts to recapitulate adult maturity, most have yet to meet the necessary benchmarks for the intended tissues. Here, we summarize the available state of knowledge surrounding the physiological mechanisms underlying cell maturation in several key tissues. Common signaling consolidators, as well as potential synergies between critical signaling pathways are explored. Finally, current practices in physiologically relevant tissue engineering and experimental design are critically examined, with the goal of integrating greater decision paradigms and frameworks towards achieving efficient maturation strategies, which in turn may produce higher-valued iPSC-derived tissues.

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On-a-Chip-Based Sensitive Detection of Drug-Induced Apoptosis in Polarized Gastric Epithelial Cells

Cited by 6 publications

References 18 publications

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Fast Protein and Metabolites (Nucleotides and Nucleosides) Liquid Chromatography Technique and Chemical Sensor for the Assessment of Fish and Meat Freshness

Harnessing conserved signaling and metabolic pathways to enhance the maturation of functional engineered tissues

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