On-Capillary Surface-Enhanced Raman Spectroscopy: Determination of Glutathione in Whole Blood Microsamples

Sánchez-Illana, Ángel; Mayr, Fritz; Cuesta-García, Daniel; Piñeiro-Ramos, José David; Cantarero, A.; Guárdia, Miguel de la; Vento, Máximo; Lendl, Bernhard; Quintás, Guillermo; Kuligowski, Julia

doi:10.1021/acs.analchem.8b01492

Cited by 49 publications

(22 citation statements)

References 54 publications

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“…GSH plays a central role in scavenging reactive oxygen species (ROS) to maintain redox homeostasis [ 37 ]. Sánchez-Illana had determined the GSH peaks by Raman spectra [ 38 ]. Among the wave numbers, a wavelength of 1647 was selected to visualize the GSH distribution.…”

Section: Resultsmentioning

confidence: 99%

The Endophytic Fungus Piriformospora Indica-Assisted Alleviation of Cadmium in Tobacco

Zeng

Liu

et al. 2021

JoF

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Increasing evidence suggests that the endophytic fungus Piriformospora indica helps plants overcome various abiotic stresses, especially heavy metals. However, the mechanism of heavy metal tolerance has not yet been elucidated. Here, the role of P. indica in alleviating cadmium (Cd) toxicities in tobacco was investigated. It was found that P. indica improved Cd tolerance to tobacco, increasing Cd accumulation in roots but decreasing Cd accumulation in leaves. The colonization of P. indica altered the subcellular repartition of Cd, increasing the Cd proportion in cell walls while reducing the Cd proportion in membrane/organelle and soluble fractions. During Cd stress, P. indica significantly enhanced the peroxidase (POD) activity and glutathione (GSH) content in tobacco. The spatial distribution of GSH was further visualized by Raman spectroscopy, showing that GSH was distributed in the cortex of P. indica-inoculated roots while in the epidermis of the control roots. A LC-MS/MS-based label-free quantitative technique evaluated the differential proteomics of P. indica treatment vs. control plants under Cd stress. The expressions of peroxidase, glutathione synthase, and photosynthesis-related proteins were significantly upregulated. This study provided extensive evidence for how P. indica enhances Cd tolerance in tobacco at physiological, cytological, and protein levels.

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Section: Resultsmentioning

confidence: 99%

The Endophytic Fungus Piriformospora Indica-Assisted Alleviation of Cadmium in Tobacco

Zeng

Liu

et al. 2021

JoF

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“…Surface-enhanced Raman spectroscopy (SERS) (Sánchez-Illana et al, 2018;Yao et al, 2019) is also gaining a lot of attention in recent years because of its great potential in detection of very small concentrations of analytes (down to a single molecule detection). The detection and quantitative determination of GSH have been one of the direct applications of many of the aforementioned new approaches in fluorescence spectroscopy and SERS.…”

Section: Spectroscopic Probes and Sensorsmentioning

confidence: 99%

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

Hamad

Elshahawy

Negm

et al. 2020

Reviews in Analytical Chemistry

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AbstractGlutathione is a natural tripeptide that plays a major role in different physiological processes in the human body. Determination of glutathione in different body fluids and tissues is essential for early diagnosis and follow-up of various diseases. The assay of glutathione is problematic because of the high polarity, the limited stability, and the aliphatic structure, which lacks the appropriate chromophore for UV detection. A number of methods have been reported for determination of glutathione using different techniques. High-performance liquid chromatography was employed in both reverse phase and hydrophilic interaction modes. Electrochemical methods exploited the redox activity of glutathione to allow for quantification by different electrodes after chemical modification, including glassy carbon, carbon paste, and nanocomposite electrodes. Capillary zone electrophoresis was used with less need for derivatization which makes it simpler, faster, and more economic. A number of nanosensors and probes have been developed to assay glutathione in biological fluids using semiconductor nanoparticles, quantum dots, genetically engineered green fluorescent probes, and new derivatives of known dye classes. This work is an updated review of the methods of analysis of glutathione and glutathione disulfide in pharmaceuticals and biological fluids with more emphasis on the technical problems and the assay artifacts.

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“…We have performed analytical monitoring with high reliability by using a silver colloid that enhances the GSH signal and allows for the accurate measurement of microvolumes (20 µL) of blood. This novel analytical tool has been validated and is extremely suitable, using a portable optical sensor device to perform point-of-care (POC) testing of OS levels in newborns [58].…”

Section: Biomarkers Of Oxidative Stress Employed In the Clinical Smentioning

confidence: 99%

Oxidative Stress in the Newborn Period: Useful Biomarkers in the Clinical Setting

Millán¹,

Piñero-Ramos²,

Lara³

et al. 2018

Antioxidants

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Aerobic metabolism is highly efficient in providing energy for multicellular organisms. However, even under physiological conditions, an incomplete reduction of oxygen produces reactive oxygen species and, subsequently, oxidative stress. Some of these chemical species are highly reactive free radicals capable of causing functional and structural damage to cell components (protein, lipids, or nucleotides). Oxygen is the most used drug in ill-adapted patients during the newborn period. The use of oxygen may cause oxidative stress-related diseases that increase mortality and cause morbidity with adverse long-term outcomes. Conditions such as prematurity or birth asphyxia are frequently treated with oxygen supplementation. Both pathophysiological situations of hypoxia–reoxygenation in asphyxia and hyperoxia in premature infants cause a burst of reactive oxygen species and oxidative stress. Recently developed analytical assays using mass spectrometry have allowed us to determine highly specific biomarkers with minimal samples. The detection of these metabolites will help improve the diagnosis, evolution, and response to therapy in oxidative stress-related conditions during the newborn period.

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On-Capillary Surface-Enhanced Raman Spectroscopy: Determination of Glutathione in Whole Blood Microsamples

Cited by 49 publications

References 54 publications

The Endophytic Fungus Piriformospora Indica-Assisted Alleviation of Cadmium in Tobacco

The Endophytic Fungus Piriformospora Indica-Assisted Alleviation of Cadmium in Tobacco

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

Oxidative Stress in the Newborn Period: Useful Biomarkers in the Clinical Setting

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