On-Chip Liquid Chromatography

Tsunoda, Makoto

doi:10.3390/encyclopedia2010041

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…Interfacing chip-LC to MS could be done either by integrating an electrospray emitter on the same chip or by tubing connection to the ESI source of a MS instrument ( Oedit et al, 2015 ). As reviewed before ( Tsunoda, 2022 ), chip-LC technology requires low sample volume, reduces reagent consumption and total cycle time (due to its miniaturized nature), and enables high throughput as well as fast analysis. Yin et al , ( Yin et al, 2005 ), used Chip-LC-ESI-MS and developed a microchip integration system on a single-chip device.…”

Section: Hyphenation Of Organ-on-a-chip and Mass Spectrometrymentioning

confidence: 99%

Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry

Hadavi,

Tosheva,

Siegel

et al. 2023

Front. Bioeng. Biotechnol.

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Three-dimensional (3D) cell cultures, including organ-on-a-chip (OOC) devices, offer the possibility to mimic human physiology conditions better than 2D models. The organ-on-a-chip devices have a wide range of applications, including mechanical studies, functional validation, and toxicology investigations. Despite many advances in this field, the major challenge with the use of organ-on-a-chips relies on the lack of online analysis methods preventing the real-time observation of cultured cells. Mass spectrometry is a promising analytical technique for real-time analysis of cell excretes from organ-on-a-chip models. This is due to its high sensitivity, selectivity, and ability to tentatively identify a large variety of unknown compounds, ranging from metabolites, lipids, and peptides to proteins. However, the hyphenation of organ-on-a-chip with MS is largely hampered by the nature of the media used, and the presence of nonvolatile buffers. This in turn stalls the straightforward and online connection of organ-on-a-chip outlet to MS. To overcome this challenge, multiple advances have been made to pre-treat samples right after organ-on-a-chip and just before MS. In this review, we summarised these technological advances and exhaustively evaluated their benefits and shortcomings for successful hyphenation of organ-on-a-chip with MS.

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Section: Hyphenation Of Organ-on-a-chip and Mass Spectrometrymentioning

confidence: 99%

Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry

Hadavi,

Tosheva,

Siegel

et al. 2023

Front. Bioeng. Biotechnol.

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“…This approach was put through as the initial model of Chip-LC, in 1990, Manz et al published their research that an open tubular column was fabricated however separation was not performed and the channel wall of the chip is taken as the stationary phase after direct modification to yield the required ability. Though back pressure is low, the main disadvantage is the low capacity of the column due to the low surface-to-volume ratio [11][12][13][14][15].…”

Section: Open Tubular Columnmentioning

confidence: 99%

Ultrasensitive Chip-Liquid Chromatography Analytical Tool for Quantification-A Review

Bhavyasri,

Srija,

Sumakanth

2023

AJACR

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Introduction: Chip liquid chromatography (LC) is a form of liquid chromatography (LC) that has been scaled down to suit a microchip for quick, high-throughput analysis with tiny sample volumes and minimal reagent usage. The term also goes by To date, four distinct on-chip LC strategies have been created. These strategies make use of columns that are open-tubular, packed-particle, monolithic, and pillar array. Applications: This technique is used to analyze small compounds and medications in a variety of biological materials, as well as for proteomics. High quantities of other endogenous chemicals that may cause interference are present alongside these biological compounds, which are only present in tiny levels. LC miniaturization offers a solution to such problems. Advantage: On-a-chip operations like sampling, sample transfer, separation, and identification are carried out. Sample preparation is the most challenging task at the micro level and is an important component of most analytical techniques, particularly in bioanalysis. One of the crucial steps in liquid chromatography is separation. Sensitive detection methods such as laser-induced fluorescence microscopy and mass spectrometry are being used.

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“…However, the separation efficiency of such columns is limited because the particles cannot be packed perfectly and uniformly [ 7 , 8 ]. To overcome this problem, pillar array columns with a completely uniform internal structure and regularly arranged microsized pillars have recently been developed [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Pillar array columns can significantly reduce eddy diffusion and lead to better resolution than particle-packed columns do.…”

Section: Introductionmentioning

confidence: 99%

“…Several types of microfluidic separation channels such as nano LC and capillary LC have also been developed in recent years. Nevertheless, pillar array columns have superior separation ability as compared to the former [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility

2022

Self Cite

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In our previous study, we developed an automatic sample injection system for pillar array columns for quantitative analysis. An autosampler was used to maintain a constant sample injection volume. However, the sample was diluted during injection using the autosampler, thus deteriorating the analytical reproducibility. In this study, we have substituted the autosampler with a syringe pump to overcome the abovementioned problem and improve the system. Sample dilution was avoided by filling the entire capillary with the sample at a constant rate. This improved system also increased the analytical reproducibility. In the previous system, the relative standard deviation (RSD) exceeded 17% of the peak height for coumarin dyes. In contrast, the improved system decreased the RSD to the range 1.2–1.8%. The analytical reproducibility was evaluated by using five types of amino acids. The RSD of each peak height was within 3.0%, confirming good reproducibility. These results indicate that the sample injection method developed in this study can be applied to biological sample analyses as a simple quantitative analysis method for pillar array columns.

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On-Chip Liquid Chromatography

Cited by 5 publications

References 36 publications

Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry

Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry

Ultrasensitive Chip-Liquid Chromatography Analytical Tool for Quantification-A Review

Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility

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