On-chip miRNA extraction platforms: recent technological advances and implications for next generation point-of-care nucleic acid tests

Petrou, Loukia; Ladame, Sylvain

doi:10.1039/d1lc00868d

Cited by 12 publications

(10 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the absence of standardized protocol, this preprocessing is a commonly accepted source of error and variation between studies and remains one of the main bottlenecks in the development of POC tests based on miR detection. [25,[32][33][34] Herein, we are reporting on the design and preclinical validation of a versatile and easily tuneable LFT for the amplification-free detection of miR-150-5p, a predictive biomarker for PTB, directly from human plasma.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…[22,23] However, this was directly contradicted by other work showing that miRs in saliva and serum are mainly within exosomes, [24] highlighting not only the intricacy of the field but also the inconsistency in the literature due to the lack of standardized protocols for miR extraction and detection. [25] Recently developed miR-sensing strategies enabled the detection of endogenous concentrations of these circulating biomarkers without the need for any enzyme-catalyzed target amplification steps, preventing errors associated with amplification biases. This includes single-molecule detection nanotechnologies which, despite ultrahigh sensitivity, often require expensive equipment not easily amenable to miniaturization.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lateral Flow Test (LFT) Detects Cell‐Free MicroRNAs Predictive of Preterm Birth Directly from Human Plasma

Petrou

Latvanen

Seichepine

et al. 2022

Advanced NanoBiomed Research

Self Cite

View full text Add to dashboard Cite

Despite extensive research toward the development of point‐of‐care nucleic acid tests (POC NATs) for the detection of microRNAs (miRs) from liquid biopsies, major hurdles remain including the strict requirement for extensive off‐chip sample preprocessing. Herein, a nucleic acid lateral flow test (NALFT) is reported on that enables the direct detection of endogenous miRs from as little as 3 μL of plasma without the requirement for any enzyme‐catalyzed target amplification or complex miR extraction steps. This is achieved through integration of a denaturing hydrogel composite material onto the LFT, allowing for near‐instantaneous on‐chip release of miRs from their carriers (extracellular vesicles or transport proteins) prior to detection. This next‐generation LFT is sensitive enough to detect endogenous concentrations of miR‐150‐5p, a predictive biomarker for preterm birth (PTB) found deregulated in maternal blood from as early as 12th week of pregnancy. Herein, a key step is represented toward a first bedside test for risk‐stratification during pregnancy by predicting true outcome at a very early stage. More generally, the universal and versatile nature of this novel sample preprocessing platform can further improve the robustness of existing NALFTs and facilitate their application at the POC.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lateral Flow Test (LFT) Detects Cell‐Free MicroRNAs Predictive of Preterm Birth Directly from Human Plasma

Petrou

Latvanen

Seichepine

et al. 2022

Advanced NanoBiomed Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…21−23 Point-of-Care Testing (POCT) is rapidly advancing in the field of miRNA detection. 24,25 Among these, Lateral Flow Assays offer advantages such as simple operation, cost-effectiveness, portability, and a short detection time. They are suitable for medical point-of-care environments or regions with limited resources.…”

Section: Introductionmentioning

confidence: 99%

AI-Enhanced Visual-Spectral Synergy for Fast and Ultrasensitive Biodetection of Breast Cancer-Related miRNAs

Wang,

Liu,

Zhu

et al. 2024

ACS Nano

View full text Add to dashboard Cite

In biomedical testing, artificial intelligence (AI)enhanced analysis has gradually been applied to the diagnosis of certain diseases. This research employs AI algorithms to refine the precision of integrative detection, encompassing both visual results and fluorescence spectra from lateral flow assays (LFAs), which signal the presence of cancer-linked miRNAs. Specifically, the color shift of gold nanoparticles (GNPs) is paired with the red fluorescence from nitrogen vacancy color centers (NVcenters) in fluorescent nanodiamonds (FNDs) and is integrated into LFA strips. While GNPs amplify the fluorescence of FNDs, in turn, FNDs enhance the color intensity of GNPs. This reciprocal intensification of fluorescence and color can be synergistically augmented with AI algorithms, thereby improving the detection sensitivity for early diagnosis. Supported by the detection platform based on this strategy, the fastest detection results with a limit of detection (LOD) at the fM level and the R 2 value of ∼0.9916 for miRNA can be obtained within 5 min. Meanwhile, by labeling the capture probes for miRNA-21 and miRNA-96 (both of which are early indicators of breast cancer) on separate T-lines, simultaneous detection of them can be achieved. The miRNA detection methods employed in this study may potentially be applied in the future for the early detection of breast cancer.

show abstract

“…Since then, vast improvement has occurred in isothermal amplification methods, which calls for an update. Other recent reviews have focused on different issues concerning related topics, such as on-chip cell lysis ( Grigorov et al, 2021 ; Chen Y. et al, 2022 ; Petrou and Ladame, 2022 ), the role of magnetic beads in these steps ( Chen et al, 2020a ), on-chip PCR methods ( Chen S. et al, 2022 ), the contribution of 3D printing to on-chip amplification ( Tzivelekis et al, 2021 ) and detection methods for all-in-one systems ( Li et al, 2021 ), and microfluidic sensors ( Sharma and Sharma, 2022 ). Several other reviews have also focused on these steps without limiting their scope to on-chip methods; these have covered topics such as sample preparation ( Jeon et al, 2018 ; Danaeifar, 2022 ), isothermal amplification ( Obande and Singh, 2020 ; Pumford et al, 2020 ; Islam and Koirla, 2021 ; Maiti et al, 2022 ), and detection ( Chiorcea-Paquim and Oliveira-Brett, 2021 ; Leonardo et al, 2021 ; Reyes et al, 2021 ; Bhat et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Wang

Jiang

Pan

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

As nucleic acid testing is playing a vital role in increasingly many research fields, the need for rapid on-site testing methods is also increasing. The test procedure often consists of three steps: Sample preparation, amplification, and detection. This review covers recent advances in on-chip methods for each of these three steps and explains the principles underlying related methods. The sample preparation process is further divided into cell lysis and nucleic acid purification, and methods for the integration of these two steps on a single chip are discussed. Under amplification, on-chip studies based on PCR and isothermal amplification are covered. Three isothermal amplification methods reported to have good resistance to PCR inhibitors are selected for discussion due to their potential for use in direct amplification. Chip designs and novel strategies employed to achieve rapid extraction/amplification with satisfactory efficiency are discussed. Four detection methods providing rapid responses (fluorescent, optical, and electrochemical detection methods, plus lateral flow assay) are evaluated for their potential in rapid on-site detection. In the final section, we discuss strategies to improve the speed of the entire procedure and to integrate all three steps onto a single chip; we also comment on recent advances, and on obstacles to reducing the cost of chip manufacture and achieving mass production. We conclude that future trends will focus on effective nucleic acid extraction via combined methods and direct amplification via isothermal methods.

show abstract

On-chip miRNA extraction platforms: recent technological advances and implications for next generation point-of-care nucleic acid tests

Abstract: Circulating microRNAs (or miRNAs) in bodily fluids, are increasingly being highlighted as promising diagnostic and predictive biomarkers for a broad range of pathologies. Although nucleic acid sensors have been developed...

Cited by 12 publications

References 79 publications

Lateral Flow Test (LFT) Detects Cell‐Free MicroRNAs Predictive of Preterm Birth Directly from Human Plasma

Lateral Flow Test (LFT) Detects Cell‐Free MicroRNAs Predictive of Preterm Birth Directly from Human Plasma

AI-Enhanced Visual-Spectral Synergy for Fast and Ultrasensitive Biodetection of Breast Cancer-Related miRNAs

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Contact Info

Product

Resources

About