On Choosing Control Objects in Experimental Gerontological Research

Хохлов, А. Н.; Клебанов, А. А.; Моргунова, Г. В.

doi:10.3103/s0096392518020049

Cited by 22 publications

(12 citation statements)

References 28 publications

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“…16,17 Only the experiments with knockout of genes that responsible for metabolic regulators look unambiguous. On the one hand, caloric restriction makes it possible to extend lifespan, on the other hand, maturation process is slower, and individuals grown up on such rations turn out to be smaller than the control.…”

Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

“…34 However, metformin, that causes AMPK activation, does not increase lifespan in male and female Drosophila melanogaster and only reduced lipid stores. 17 When it comes to clinical trials of metformin, there are no enough statistically reliable data available to make a conclusion. The authors reasonably note that the researchers often perform experiments with metformin on animals with genetic defects, including the animals used as models for human diseases, for example, Huntington disease in mice 36 ; at the same time, the effect of the drug on healthy mice and rats is not tested.…”

Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

“…It was repeatedly assumed that ad libitum food intake is not normal because animals in wild are kept on a more strict diet than in the laboratory. 16,17 Only the experiments with knockout of genes that responsible for metabolic regulators look unambiguous. Animals with knockout of AMPKα as a rule die earlier than wild-type animals.…”

Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

“…This problem of choosing control group in gerontological research we considered earlier. 17 When it comes to clinical trials of metformin, there are no enough statistically reliable data available to make a conclusion. 34 The results are only for patients with type II diabetes, while it remains unclear how the drug would affect healthy volunteers.…”

Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

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Age‐related AMP‐activated protein kinase alterations: From cellular energetics to longevity

Моргунова

Клебанов

2019

Cell Biochemistry & Function

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Funding informationRussian Foundation for Basic Research, Grant/ Award Number: 18-34-00813 mol_a 5' adenosine monophosphate-activated protein kinase (AMPK) is a key regulator of energy in the cell, which allows the cell/organism to survive with deficit of ATP. Since AMPK is involved in the adaptation to caloric restriction, the role of age-related changes in AMPK activity in both the aging organism and the aging cell is actively investigated in gerontology. Studies on yeast, worms, flies, rodents, and primates have demonstrated an important effect of this regulator on key signalling pathways involved in the aging process. In some cases, researchers conclude that AMPK promotes aging. However, in our opinion, in such cases, we observe a disturbance in the adaptive ability because of the prolonged cell/organism presence in stressful conditions because the functional capacity of any adaptation system is limited. Interestingly, AMPK can regulate metabolic processes in noncell-autonomous manner. The main effects of AMPK activation in the cell are realized in restriction of proliferation and launching autophagy. In tissues of an aging organism, the ability of AMPK to respond to energy deficit decreases; this fact is especially critical for organs that contain postmitotic cells. In this review, we have tried to consider the involvement of AMPK in age-related changes in the cell and in the organism.

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Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

Section: Role Of Age-related Changes In Ampk Activity In the Organismmentioning

confidence: 99%

See 2 more Smart Citations

Age‐related AMP‐activated protein kinase alterations: From cellular energetics to longevity

Моргунова

Клебанов

2019

Cell Biochemistry & Function

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show abstract

“…В частности, рост однозначно будет различаться для двух культур, находящихся на разных пассажах, а от характера роста напрямую зависит вымирание культуры. В связи с этим, лучше использовать трансформированные или иммортализованные клетки, не имеющие пролиферативных ограничений [20].…”

Section: материалы и методыunclassified