On-column conductivity detector for capillary zone electrophoresis

Huang, Xiaoxia; Pang, Teng Ke J.; Gordon, Manuel J.; Zare, Richard N.

doi:10.1021/ac00150a004

Cited by 201 publications

(103 citation statements)

References 4 publications

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“…Several types of CD have been proposed [296][297][298][299]. Analytical grade CDs utilizing an AC impedance measurement allow the avoidance of artifacts of sensor surface polarization and interferences from the galvanically coupled HV source.…”

Section: Contact and Contactless Conductivity Detection (Cd Ccd C 4mentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

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Section: Contact and Contactless Conductivity Detection (Cd Ccd C 4mentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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“…There were very few publications in the 1970's and 1980's [18,65,66]. The 1990's have shown some activity in this area with an increase in the number of publications.…”

Section: Separation Of Inorganic Cations By Capillary Electrophoresismentioning

confidence: 99%

Separation of ions in acidic solution by capillary electrophoresis

Thornton

1997

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“…7 Conductometric detection (CD) offers a good alternative for determining small ions separated by FSCE. [8][9][10][11][12][13][14][15][16][17] In this mode of detection, the response is proportional to the difference of mobilities between the analyte and the running electrolyte co-ion. In general, the greater the mobility difference, the greater the sensitivity achieved.…”

Section: Introductionmentioning

confidence: 99%

Improved separation of IA and IIA metal cations in matrices with high sodium concentration by capillary electrophoresis with contactless conductometric detection

Silva

Ricelli

Carvalho

et al. 2003

J. Braz. Chem. Soc.

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Um dos usos mais comuns da eletroforese capilar com detecção condutométrica é a análise de vários tipos de amostras contendo metais alcalinos e alcalinos terrosos. No entanto, a alta concentração de sódio, a qual é uma ocorrência bastante comum, pode causar perda de resolução de alguns picos. Neste trabalho, as condições e o eletrólito de corrida conveniente para análises de soro e fluído para hemodiálise são apresentados. A idéia básica é deslocar os picos de Ca 2+ e Mg 2+ pela redução de suas mobilidades pela adição de lactato e metanol ao eletrólito de corrida. Limites de detecção da ordem de 0,5 a 1,0 µmol L -1 e razão molar Na + /Ca 2+ da ordem de 1000 foram obtidos. Estas condições excedem as necessidades da aplicação pretendida.One of the most common uses of capillary electrophoresis with conductometric detection is the analysis of different samples containing alkaline and alkaline earth metals. However, the high sodium content, which is a very common occurrence, may cause loss of resolution of some peaks. In this work, the conditions and a running buffer suitable for serum and hemodialysis fluid analyses are presented. The approach basically consists in the reduction of mobilities of Ca 2+ and Mg 2+ by addition of methanol and lactate in the running buffer. Limits of detection in the range from 0.5 to 1.0 µmol L -1 with molar Na + /Ca 2+ ratios as high as 1000 were obtained, which are conditions that exceed the requirements of the proposed determination.Keywords: capillary electrophoresis, contactless conductivity detection, human serum, hemodialysis fluid IntroductionMetal cations from groups IA and IIA play an important role in numerous processes in the human body, such as volume and osmotic regulation, myocardial rhythm, blood coagulation, neuromuscular excitability, as cofactors in enzyme activation, in the regulation of ATPase ion pumps, and so on. The reference ranges for potassium, sodium, calcium, and magnesium in serum of normal individuals are 3.4-5.0, 135-145, 0.65-1.0, and 2.15-2.65 mmol L -1 , respectively. Abnormal concentration values for these ions can help in the diagnosis of many metabolism disorders, such as diabetes, advanced renal disease, polydipsia, aldosterone deficiency or excess, cardiac disorders, alcoholism etc. 1 Capillary electrophoresis (CE) is a separation technique based on differences of mobility of charged species and has been applied for determination of several kinds of analytes, from small ionic species to macromolecules. 2,3 Metal cations from groups IA and IIA have been mainly analyzed by free solution capillary electrophoresis (FSCE) with indirect UV-visible absorption detection. [4][5][6][7] Although these cations can be readily separated at low concentrations (typically 10 -6 mol L -1 ), separations of concentrated samples require the use of complexating agents to selectively modify the ion mobility and improve resolution. Examples of ligands widely used in FSCE separations include carboxylic acids 4-6 -such as oxalic, tartaric, lactic, α-hydroxyisobutyric (HIBA...

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On-column conductivity detector for capillary zone electrophoresis

Abstract: A new on-column conductivity detector tor use with capillary zone electrophoresis Is described. The system, constructed with Inexpensive, readily available components, can analyze nanolter samples. Peak area Is linear with concentration over

Cited by 201 publications

References 4 publications

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Separation of ions in acidic solution by capillary electrophoresis

Improved separation of IA and IIA metal cations in matrices with high sodium concentration by capillary electrophoresis with contactless conductometric detection

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