A simulation model based on satellite observations of monthly vegetation cover from the Moderate Resolution Imaging Spectroradiometer (MODIS) was used to estimate monthly carbon fluxes in terrestrial ecosystems of the conterminous United States over the period 2001-04. United States was estimated as annual net sink of about +0.2 Pg C in 2004. Regional climate patterns were reflected in the predicted annual NEP flux from the model, which showed extensive carbon sinks in ecosystems of the southern and eastern regions in 2003-04, and major carbon source fluxes from ecosystems in the Rocky Mountain and Pacific Northwest regions in 2003-04. As demonstrated through tower site comparisons, net primary production (NPP) modeled with monthly MODIS enhanced vegetation index (EVI) inputs closely resembles both the measured high-and low-season carbon fluxes. Modeling results suggest that the capacity of the NASA Carnegie Ames Stanford Approach (CASA) model to use 8-km resolution MODIS EVI data to predict peak growing season uptake rates of CO 2 in irrigated croplands and moist temperate forests is strong.