On-line biosensor for the detection of putative toxicity in water contaminants

Eltzov, Evgeni; Slobodník, Vladimír; Ionescu, Rodica Elena; Marks, Robert S.

doi:10.1016/j.talanta.2014.09.032

Cited by 22 publications

(6 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such effect was also observed with the TV1061 strain, a bacterium sensitive to general cytotoxic damages. The sensitivity and reliability of this strain have been proven in many different applications for air [ 81 ], soil [ 82 ] and water [ 83 ] toxicity monitoring. In this study, the induction effect of SS1, SS5, SS7, and SS10 supplements on TV1061 indicates the activation of the cytotoxicity repair mechanisms in the cells, and therefore provides data on their possible toxicity mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Measuring Artificial Sweeteners Toxicity Using a Bioluminescent Bacterial Panel

Harpaz

Yeo

Cecchini³

et al. 2018

Molecules

Self Cite

View full text Add to dashboard Cite

Artificial sweeteners have become increasingly controversial due to their questionable influence on consumers’ health. They are introduced in most foods and many consume this added ingredient without their knowledge. Currently, there is still no consensus regarding the health consequences of artificial sweeteners intake as they have not been fully investigated. Consumption of artificial sweeteners has been linked with adverse effects such as cancer, weight gain, metabolic disorders, type-2 diabetes and alteration of gut microbiota activity. Moreover, artificial sweeteners have been identified as emerging environmental pollutants, and can be found in receiving waters, i.e., surface waters, groundwater aquifers and drinking waters. In this study, the relative toxicity of six FDA-approved artificial sweeteners (aspartame, sucralose, saccharine, neotame, advantame and acesulfame potassium-k (ace-k)) and that of ten sport supplements containing these artificial sweeteners, were tested using genetically modified bioluminescent bacteria from E. coli. The bioluminescent bacteria, which luminesce when they detect toxicants, act as a sensing model representative of the complex microbial system. Both induced luminescent signals and bacterial growth were measured. Toxic effects were found when the bacteria were exposed to certain concentrations of the artificial sweeteners. In the bioluminescence activity assay, two toxicity response patterns were observed, namely, the induction and inhibition of the bioluminescent signal. An inhibition response pattern may be observed in the response of sucralose in all the tested strains: TV1061 (MLIC = 1 mg/mL), DPD2544 (MLIC = 50 mg/mL) and DPD2794 (MLIC = 100 mg/mL). It is also observed in neotame in the DPD2544 (MLIC = 2 mg/mL) strain. On the other hand, the induction response pattern may be observed in its response in saccharin in TV1061 (MLIndC = 5 mg/mL) and DPD2794 (MLIndC = 5 mg/mL) strains, aspartame in DPD2794 (MLIndC = 4 mg/mL) strain, and ace-k in DPD2794 (MLIndC = 10 mg/mL) strain. The results of this study may help in understanding the relative toxicity of artificial sweeteners on E. coli, a sensing model representative of the gut bacteria. Furthermore, the tested bioluminescent bacterial panel can potentially be used for detecting artificial sweeteners in the environment, using a specific mode-of-action pattern.

show abstract

Section: Discussionmentioning

confidence: 99%

Measuring Artificial Sweeteners Toxicity Using a Bioluminescent Bacterial Panel

Harpaz

Yeo

Cecchini³

et al. 2018

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cytotoxic substances present in tap water could also be detected using bioluminescent E. coli bio-reporter strain TV1061 via integration of specific heat-shock grpe promoter with luxCDABE reporter operon [377]. The bioassays and microbial biosensors employed for the toxicity assessment involved Chlorella sp., Chlorella vulgaris, Monoraphidium sp., Scenedesmus subspicatus and Brachionus calyciflorus sp.…”

Section: Biosensorsmentioning

confidence: 99%

“…Initially, the model-based event detection method involves a signal-to-noise principles using laboratory and sensor test-loop evaluation. Indication of contamination events is derived from the chemical changes in background water quality signals [88,89], [137][138][139][140][141][142][143], [163], [167], [217], [244,245], [429,430] Sensor Placements Approach [372][373][374][375][376][377], [380][381][382][383][384][385][386][387][388], [391], [400][401][402][403][404][405][406][407], [434][435][436][437][438] Event Detection Model-based -High true positive alarm rate -Low false alarm detections -Fast response time -Complicated calibration process -Highly dependable on predictions and estimations -Computationally intensive…”

Section: Algorithmic Model-based Event Detectionmentioning

confidence: 99%

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

Zulkifli

Rahim

Lau

2018

Sensors and Actuators B: Chemical

232

View full text Add to dashboard Cite

a b s t r a c tWater monitoring technologies are widely used for contaminants detection in wide variety of water ecology applications such as water treatment plant and water distribution system. A tremendous amount of research has been conducted over the past decades to develop robust and efficient techniques of contaminants detection with minimum operating cost and energy. Recent developments in spectroscopic techniques and biosensor approach have improved the detection sensitivities, quantitatively and qualitatively. The availability of in-situ measurements and multiple detection analyses has expanded the water monitoring applications in various advanced techniques including successful establishment in hand-held sensing devices which improves portability in real-time basis for the detection of contaminant, such as microorganisms, pesticides, heavy metal ions, inorganic and organic components. This paper intends to review the developments in water quality monitoring technologies for the detection of biological and chemical contaminants in accordance with instrumental limitations. Particularly, this review focuses on the most recently developed techniques for water contaminant detection applications. Several recommendations and prospective views on the developments in water quality assessments will also be included.

show abstract

“…This system has been successfully used for continuous long-term monitoring and early warning of MC-LR, which is a toxin formed by cyanobacteria, in Lake Tai (China). Researchers have also developed a prototype online fibre-optic biosensing system based on immobilized bioreporter bacteria for monitoring water pollutants (Eltzov, Slobodnik, Ionescu, & Marks, 2015). A schematic diagram of this biosensor and its principle of operation are shown in Figure 12.…”

Section: Online Sensing Technologies For Monitoring Water Qualitymentioning

confidence: 99%

“…A. Schematic representation of the perspex flow unit of the on-line fibre-optic biosensing system based on immobilized bioreporter bacteria for monitoring water pollutants consisting of a fiber optic probe and peristaltic pumps; B. Photograph of perspex flow unit; and C. Calcium-polymerized alginate with six adlayers (adapted from(Eltzov et al, 2015)).…”

mentioning

confidence: 99%

Biosensors for on-line water quality monitoring – a review

Hossain

Mansour

2019

Arab Journal of Basic and Applied Sciences

View full text Add to dashboard Cite

Biosensors are analytical tools that convert a bio-signal into a measurable response. The applicability of these devices is rapidly expanding and their fabrication for continuous and real-time detection is gaining increased attention. Current research continues to further improve their features. Some of the major advantages of these devices are that they are portable and easy to operate, provide a rapid response, can be miniaturized, have a long-life cycle and a short response time and possess a high sensitivity, selectivity and specificity. Biosensors are more efficient and economical than conventional detection methods for a wide range of applications. This article is a tutorial review on the basic concepts of biosensors and their application in water quality monitoring, especially on-line detection systems. The following topics are discussed: (i) basic concepts of biosensors, (ii) biosensing elements and transducers, (iii) biosensor types and performance criteria and (iv) specific applications on water quality monitoring. In addition, this review highlights the various aspects of portable paper-based sensors for on-site detection of pesticides, heavy metals and bacteria in water. Online sensing technologies for water quality monitoring are preferable than offline/ onsite detecting technologies, considering that online systems provide real-time data.

show abstract

On-line biosensor for the detection of putative toxicity in water contaminants

Cited by 22 publications

References 51 publications

Measuring Artificial Sweeteners Toxicity Using a Bioluminescent Bacterial Panel

Measuring Artificial Sweeteners Toxicity Using a Bioluminescent Bacterial Panel

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

Biosensors for on-line water quality monitoring – a review

Contact Info

Product

Resources

About