On-Line Coupling of Solid Phase Extraction Sample Processing with High-Performance Liquid Chromatography

Pyrzyńska, Krystyna; Poboży, Ewa

doi:10.1080/10408340290765533

Cited by 32 publications

(18 citation statements)

References 87 publications

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“…So, a crucial aspect in hyphenated system is to possess means for the reliable delivery and the introduction of medium volume of eluate for further analysis. Online column‐switching method and sequential injection‐based programmable elution mode are efficient methods for this purpose. However, either too large volume of overall solvent or small volume with partial preconcentration solvent was used in these methods.…”

Section: Resultsmentioning

confidence: 99%

Online desorption of molecularly imprinted stir bar sorptive extraction coupled to high performance liquid chromatography for the trace analysis of triazines in rice

Zhong

et al. 2012

J of Separation Science

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Based on a special homemade interface, the molecularly imprinted stir bar sorptive extraction was coupled to high performance liquid chromatography for the online desorption and analysis. During desorption, the analytes desorbed from stir bar were delivered to a sample loop and then was introduced into liquid chromatography for further analysis. The online desorption and introduction processes were real-time monitored by the ultraviolet detector of the liquid chromatography system. In this way, the method sensitivity and reproducibility was improved for the introduction timing of the desorption solvent with greatest concentration of the target analytes was accurately controlled. To demonstrate the feasibility of the method, terbuthylazine imprinted stir bar was synthesized and used for the analysis of nine triazines in rice. Under the optimized conditions, limits of detection of 0.02-0.11 μg/L and precision within 4.3-7.2% were achieved. The new method was compared with other two traditional offline desorption procedures, i.e. ultrasonic-assisted desorption and static thermal desorption. The comparison results showed that the proposed method is accurate, precise, fast, and suitable for the trace analysis of complex samples.

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Section: Resultsmentioning

confidence: 99%

Online desorption of molecularly imprinted stir bar sorptive extraction coupled to high performance liquid chromatography for the trace analysis of triazines in rice

Zhong

et al. 2012

J of Separation Science

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“…Although many different on‐line SPE methodologies have been reported, the on‐line SPE approach has not been widely adopted for routine bioanalytical applications, primarily due to issues related to method ruggedness, sensitivity limitations, and a lack of simple and generic methods. To the best of our knowledge, all of the previously reported on‐line SPE applications are associated with a multi‐component LC pumping scheme that requires at least two individual HPLC pumping units connected by one or two switching valves (six‐ or ten‐port) 13, 17, 18, 28–30. One of the two HPLC systems is primarily used for plasma loading and washing on the SPE column, while the other is primarily for analytical separation of the compounds eluted from the SPE column after plasma proteins are removed.…”

Section: Resultsmentioning

confidence: 99%

A novel on‐line solid‐phase extraction approach integrated with a monolithic column and tandem mass spectrometry for direct plasma analysis of multiple drugs and metabolites

Zang

Luo

Song

et al. 2005

Rapid Comm Mass Spectrometry

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An on-line solid-phase extraction liquid chromatography/tandem mass spectrometry (SPE LC/MS/MS) assay using a newly developed SPE column and a monolithic column was developed and validated for direct analysis of plasma samples containing multiple analytes. This assay was developed in an effort to increase bioanalysis throughput and reduce the complexity of on-line SPE LC/MS/MS systems. A simple column-switching configuration that requires only one six-port valve and one HPLC pumping system was employed for on-line plasma sample preparation and subsequent gradient chromatographic separation. The resulting analytical method couples the desired sensitivity with ease of use. The method was found to perform satisfactorily for direct plasma analysis with respect to assay linearity, specificity, sensitivity, precision, accuracy, carryover, and short-term stability of an eight-analyte mixture in plasma. A gradient LC condition was applied to separate the eight analytes that cannot be distinctly differentiated by MS/MS. With a run time for every injection of 2.8 min, a minimum of 300 direct plasma injections were made on one on-line SPE column without noticeable changes in system performance. Due to the ruggedness and simplicity of this system, generic methods can be easily developed and applied to analyze a wide variety of compounds in a high-throughput manner without laborious off-line sample preparation.

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“…In particular, in the analysis of polar solutes the breakthrough volumes and the capacity of the cartridge may be too low to meet the required detection limits. Since a large number of SPE-LC applications have been reported and several reviews have been published on these techniques [1,2,3], the SPE-LC methods are not included in this review.…”

Section: Solid-phase Extractionmentioning

confidence: 98%

Approaches for on-line coupling of extraction and chromatography

Hyötyläinen

Riekkola

2004

Analytical and Bioanalytical Chemistry

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This review provides an overview of the approaches available in order to perform on-line coupling of various extraction techniques with liquid and gas chromatography, for the analysis of semivolatile and nonvolatile analytes in liquid and solid samples. The main focus is on the instrumental set-up of these techniques. Selected real applications are described by way of illustration. The extraction methods suitable for on-line coupling covered in this review are: liquid-liquid extraction, solid-phase extraction, membrane-based techniques, pressurised liquid extraction, supercritical fluid extraction, and microwave- and sonication-assisted extractions. The following systems are not covered in this review: on-line coupled solid-phase extraction-liquid chromatography, purge-and-trap-GC, and membrane extraction with a sorbent interface-GC.

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On-Line Coupling of Solid Phase Extraction Sample Processing with High-Performance Liquid Chromatography

Cited by 32 publications

References 87 publications

Online desorption of molecularly imprinted stir bar sorptive extraction coupled to high performance liquid chromatography for the trace analysis of triazines in rice

Online desorption of molecularly imprinted stir bar sorptive extraction coupled to high performance liquid chromatography for the trace analysis of triazines in rice

A novel on‐line solid‐phase extraction approach integrated with a monolithic column and tandem mass spectrometry for direct plasma analysis of multiple drugs and metabolites

Approaches for on-line coupling of extraction and chromatography

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