On-line trace enrichment in hyphenated liquid chromatography–nuclear magnetic resonance spectroscopy

Koning, Jesper; Hogenboom, A.C.; Lacker, Tanja; Strohschein, Sabine; Albert, Klaus; Brinkman, U.A.Th.

doi:10.1016/s0021-9673(98)00316-1

Cited by 43 publications

(14 citation statements)

References 12 publications

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“…There have been other reports that solvent gradients can cause NMR linebroadenings, but with less quantification of the limit or under different conditions [46,47]. Proposed workarounds to this problem have included stopped-flow LC-NMRÃ, SPE-NMR column trapping, and ''waiting before NMR acquisition" [47][48][49][50]; a mobile-phase compensation method has also been proposed as a way to actually increase this limit [51].…”

Section: Shimmingmentioning

confidence: 99%

Chemical-shift referencing and resolution stability in gradient LC–NMR (acetonitrile:water)

Keifer

2009

Journal of Magnetic Resonance

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Section: Shimmingmentioning

confidence: 99%

Chemical-shift referencing and resolution stability in gradient LC–NMR (acetonitrile:water)

Keifer

2009

Journal of Magnetic Resonance

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“…Solid-phase extraction (SPE) is a powerful technique for reproducible, rapid and selective sample preparation. 48 There are a number of studies on on-line coupling of SPE with LC-UV, 49,50 LC-MS 51,52 and LC-NMR 28,53 aiming at concentrating the sample before the separation. Alternatively, the use of a guard column after the LC separation to concentrate the eluted compounds prior to NMR analysis, 54 as well as the use of a disposable SPE cartridge connected to an NMR flow probe 55 have been described in order to increase the sensitivity of LC-NMR.…”

Section: Lc-spe-nmrmentioning

confidence: 99%

LC–NMR coupling technology: recent advancements and applications in natural products analysis

Exarchou

Krucker

Beek

et al. 2005

Magnetic Reson in Chemistry

173

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An overview of recent advances in nuclear magnetic resonance (NMR) coupled with separation technologies and their application in natural product analysis is given and discussed. The different modes of LC-NMR operation are described, as well as how technical improvements assist in establishing LC-NMR as an important tool in the analysis of plant-derived compounds. On-flow, stopped-flow and loop-storage procedures are mentioned, together with the new LC-SPE-NMR configuration. The implementation of mass spectrometry in LC-NMR is also useful on account of the molecular weight and fragmentation information that it provides, especially when new plant species are studied. Cryogenic technology and capillary LC-NMR are the other important recent developments. Since the plant kingdom is endless in producing potential drug candidates, development and optimization of LC-NMR techniques convert the study of natural products to a less-time-consuming task, speeding up identification.

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“…This type of on-line concentration technique can be incorporated to an LC-NMR system to improve the sensitivity of NMR detection. In fact, an on-line concentration technique using solid-phase-extraction has been employed for the LC-NMR analysis of three drug substances (De Koning et al, 1998). The use of LC-MS in conjunction with LC-NMR is also very important because the molecular weight information provided by LC-MS can significantly accelerate the structure identification process and improve the certainty of the structural assignment.…”

Section: Future Trends and Perspectivesmentioning

confidence: 99%

Hyphenated HPLC-NMR and its applications in drug discovery

Peng

2000

Biomed. Chromatogr.

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Hyphenated HPLC-NMR is a fast growing technology, allowing rapid and detailed structural characterization of unknown mixtures. The technical aspects of the technology are reviewed on the basis of system configuration, operation, solvent suppression, HPLC and NMR optimization, and detection. The combined use of HPLC-NMR and HPLC-MS is also described and discussed. Various applications of HPLC-NMR and integrated HPLC-NMR-MS in drug discovery, especially in the separation and structure elucidation of drug impurities, reaction mixtures, degradation products, in vitro and in vivo metabolites, and combinatorial library samples, are illustrated.

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On-line trace enrichment in hyphenated liquid chromatography–nuclear magnetic resonance spectroscopy

Cited by 43 publications

References 12 publications

Chemical-shift referencing and resolution stability in gradient LC–NMR (acetonitrile:water)

Chemical-shift referencing and resolution stability in gradient LC–NMR (acetonitrile:water)

LC–NMR coupling technology: recent advancements and applications in natural products analysis

Hyphenated HPLC-NMR and its applications in drug discovery

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