On-line UV-NIR spectroscopy as a process analytical technology (PAT) tool for on-line and real-time monitoring of the extraction process of Coptis Rhizome

Dai, Xuezhi; Song, Hang; Liu, Wen; Yao, Shun; Wang, Gang

doi:10.1039/c5ra23688f

Cited by 14 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 1 b shows the UV–vis spectra of BBR and BBR-AgNPs, FA-PEG, and FA-PEG@BBR-AgNPs. The major absorption peaks at 232, 266, 346, and 428 nm represented BBR; similar peaks were observed by Dai et al 27 The successful loading of BBR on the surface of AgNPs was evident from the presence of BBR-AgNPs. After interactions of BBR with AgNPs, the peaks were slightly changed from 232, 266, 346, and 428 nm to 230, 264, 348, and 428 nm, respectively, which confirmed the successful loading of BBR on the surface of the AgNPs via electrostatic interactions.…”

Section: Resultssupporting

confidence: 81%

Drug-Carrying Capacity and Anticancer Effect of the Folic Acid- and Berberine-Loaded Silver Nanomaterial To Regulate the AKT-ERK Pathway in Breast Cancer

2018

View full text Add to dashboard Cite

Currently, in clinics, breast cancer is treated with free chemotherapeutic drugs, as a result there is not much therapeutic effect in treated models, leading to substantial systemic toxicity. To overcome these critical problems for the primary outcome, we developed the formulated nanomaterial (FA-PEG@BBR-AgNPs) aimed to specifically target cancer cells via nanoscopic-based drug delivery for getting better therapeutic effectiveness. In the present study, an isoquinoline alkaloid, berberine (BBR), was chosen as a cancer therapeutic agent, encapsulated on citrate-capped silver nanoparticles (AgNPs) through electrostatic interactions (BBR-AgNPs). Then, BBR-AgNPs were conjugated with polyethylene glycol-functionalized folic acid (FA-PEG) via hydrogen bonding interactions (FA-PEG@BBR-AgNPs). The transmission electron microscopy study shows the cellular invasion of the formulated FA-PEG@BBR-AgNPs, indicating the accretion of the nanomaterial at the tumor-specific site. Hence, FA conjugated with the nanomaterial suggests an efficient release of BBR molecules into the specific cancer site. Consequently, the results showed an increase in apoptotic induction via reactive oxygen species and condensed nuclei in cancer cells. Moreover, the western blotting analysis shows reduced/increased expression of PI3K, AKT, Ras, Raf, ERK, VEGF, HIF1α, Bcl-2, Bax, cytochrome c, caspase-9, and caspase-3, thereby enhancing apoptosis. Likewise, the in vivo antitumor efficiency of FA-PEG@BBR-AgNPs showed a significant restraint of tumor progression, and histopathological observations of lung, liver, kidney, heart, and brain tissues proved lesser toxicity of FA-PEG@BBR-AgNPs. Thus, the successfully formulated nanomaterial can serve as a potential drug-discharging vehicle to combat cancer cells by a molecular-based targeting approach.

show abstract

Section: Resultssupporting

confidence: 81%

Drug-Carrying Capacity and Anticancer Effect of the Folic Acid- and Berberine-Loaded Silver Nanomaterial To Regulate the AKT-ERK Pathway in Breast Cancer

2018

View full text Add to dashboard Cite

show abstract

“…Many other instruments and approaches have been developed for the quality evaluation of CR, such as capillary electrophoresis, ultraviolet-near infrared (UV–NIR) spectroscopy [ 59 ], Fourier transform infrared (FT-IR) spectroscopy, Raman scattering spectroscopy, as well as other methods.…”

Section: Quality Evaluation Of Crmentioning

confidence: 99%

Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity

et al. 2018

View full text Add to dashboard Cite

BackgroundCoptidis rhizoma (CR) is the dried rhizome of Coptis chinensis Franch., C. deltoidea C. Y. Cheng et Hsiao or C. teeta Wall. (Ranunculaceae) and is commonly used in Traditional Chinese Medicine for the treatment of various diseases including bacillary dysentery, typhoid, tuberculosis, epidemic cerebrospinal meningitis, empyrosis, pertussis, and other illnesses.MethodsA literature survey was conducted via SciFinder, ScieneDirect, PubMed, Springer, and Wiley databases. A total of 139 selected references were classified on the basis of their research scopes, including chemical investigation, quality evaluation and pharmacological studies.ResultsMany types of secondary metabolites including alkaloids, lignans, phenylpropanoids, flavonoids, phenolic compounds, saccharides, and steroids have been isolated from CR. Among them, protoberberine-type alkaloids, such as berberine, palmatine, coptisine, epiberberine, jatrorrhizine, columamine, are the main components of CR. Quantitative determination of these alkaloids is a very important aspect in the quality evaluation of CR. In recent years, with the advances in isolation and detection technologies, many new instruments and methods have been developed for the quantitative and qualitative analysis of the main alkaloids from CR. The quality control of CR has provided safety for pharmacological applications. These quality evaluation methods are also frequently employed to screen the active components from CR. Various investigations have shown that CR and its main alkaloids exhibited many powerful pharmacological effects including anti-inflammatory, anti-cancer, anti-diabetic, neuroprotective, cardioprotective, hypoglycemic, anti-Alzheimer and hepatoprotective activities.ConclusionThis review summarizes the recent phytochemical investigations, quality evaluation methods, the biological studies focusing on CR as well as its main alkaloids.

show abstract

“…As a result of the changes in the actual application requirements and the continuous advancement of technology, NIRS has gradually become a monitoring technique in areas such as food, medicine, environment [14,15] , materials [16,17] , and crop growth [18,19] . The near-infrared devices have become smaller and real-time applications and high model accuracy are required for many monitoring and analysis methods [20] . Most of the related research has occurred in the food and medicine fields.…”

Section: Introductionmentioning

confidence: 99%

Monitoring model for predicting maize grain moisture at the filling stage using NIRS and a small sample size

Wang¹,

Yang²,

Song³

et al. 2019

International Journal of Agricultural and Biological Engineering

View full text Add to dashboard Cite

The change in the maize moisture content during different growth stages is an important indicator to evaluate the growth status of maize. In particular, the moisture content during the grain-filling stage reflects the grain quality and maturity and it can also be used as an important indicator for breeding and seed selection. At present, the drying method is usually used to calculate the moisture content and the dehydration rate at the grain-filling stage, however, it requires large sample size and long test time. In order to monitor the change in the moisture content at the maize grain-filling stage using small sample set, the Bootstrap re-sampling strategy-sample set partitioning based on joint x-y distances-partial least squares (Bootstrap-SPXY-PLS) moisture content monitoring model and near-infrared spectroscopy for small sample sizes of 10, 20, and 50 were used. To improve the prediction accuracy of the model, the optimal number of factors of the model was determined and the comprehensive evaluation thresholds RVP (coefficient of determination (R 2), the root mean square error of cross-validation (RMSECV) and the root mean square error of prediction (RMSEP)) was proposed for sub-model screening. The model exhibited a good performance for predicting the moisture content of the maize grain at the filling stage for small sample set. For the sample sizes of 20 and 50, the R 2 values were greater than 0.99. The average deviations of the predicted and reference values of the model were 0.1078%, 0.057%, and 0.0918%, respectively. Therefore, the model was effective for monitoring the moisture content at the grain-filling stage for a small sample size. The method is also suitable for the quantitative analysis of different concentrations using near-infrared spectroscopy and small sample size.

show abstract

On-line UV-NIR spectroscopy as a process analytical technology (PAT) tool for on-line and real-time monitoring of the extraction process of Coptis Rhizome

Abstract: UV-NIR spectroscopy connected method as a tool for on-line and real-time monitoring of Coptis Rhizome extraction process.

Cited by 14 publications

References 31 publications

Drug-Carrying Capacity and Anticancer Effect of the Folic Acid- and Berberine-Loaded Silver Nanomaterial To Regulate the AKT-ERK Pathway in Breast Cancer

Drug-Carrying Capacity and Anticancer Effect of the Folic Acid- and Berberine-Loaded Silver Nanomaterial To Regulate the AKT-ERK Pathway in Breast Cancer

Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity

Monitoring model for predicting maize grain moisture at the filling stage using NIRS and a small sample size

Contact Info

Product

Resources

About