On measuring the diffusional water permeability of human red blood cells and ghosts by nuclear magnetic resonance

Benga, Gheorghe; Pop, Victor I.; Popescu, Octavian; Borza, Victoria

doi:10.1016/0165-022x(90)90057-j

Cited by 60 publications

(36 citation statements)

References 40 publications

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“…Diffusion results were found well in agreement with extensive literature in red blood cell permeability [59,60]. This equation simplifies for f = 1 (i.e., no ECS) to the wellknown equation derived by Tanner [61] for a stack of flat layers with characteristic length R:…”

Section: The Long Time Regimesupporting

confidence: 78%

Time-Dependent Diffusion MRI in Cancer: Tissue Modeling and Applications

Reynaud

2017

Front. Phys.

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In diffusion weighted imaging (DWI), the apparent diffusion coefficient (ADC) has been recognized as a useful and sensitive surrogate for cell density, paving the way for non-invasive tumor staging, and characterization of treatment efficacy in cancer. However, microstructural parameters, such as cell size, density and/or compartmental diffusivities affect diffusion in various fashions, making of conventional DWI a sensitive but non-specific probe into changes happening at cellular level. Alternatively, tissue complexity can be probed and quantified using the time dependence of diffusion metrics, sometimes also referred to as temporal diffusion spectroscopy when only using oscillating diffusion gradients. Time-dependent diffusion (TDD) is emerging as a strong candidate for specific and non-invasive tumor characterization. Despite the lack of a general analytical solution for all diffusion times/frequencies, TDD can be probed in various regimes where systems simplify in order to extract relevant information about tissue microstructure. The fundamentals of TDD are first reviewed (a) in the short time regime, disentangling structural and diffusive tissue properties, and (b) near the tortuosity limit, assuming weakly heterogeneous media near infinitely long diffusion times. Focusing on cell bodies (as opposed to neuronal tracts), a simple but realistic model for intracellular diffusion can offer precious insight on diffusion inside biological systems, at all times. Based on this approach, the main three geometrical models implemented so far (IMPULSED, POMACE, VERDICT) are reviewed. Their suitability to quantify cell size, intra-and extracellular spaces (ICS and ECS) and diffusivities are assessed. The proper modeling of tissue membrane permeability-hardly a newcomer in the field, but lacking applications-and its impact on microstructural estimates are also considered. After discussing general issues with tissue modeling and microstructural parameter estimation (i.e., fitting), potential solutions are detailed. The in vivo applications of this new, non-invasive, specific approach in cancer are reviewed, ranging from the characterization of gliomas in rodent brains and observation of time-dependence in breast tissue lesions and prostate cancer, to the recent preclinical evaluation of new treatments efficacy. It is expected that clinical applications of TDD will strongly benefit the community in terms of non-invasive cancer screening.

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Section: The Long Time Regimesupporting

confidence: 78%

Time-Dependent Diffusion MRI in Cancer: Tissue Modeling and Applications

Reynaud

2017

Front. Phys.

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“…Most work has been done on erythrocytes, 52 where the membrane permeabilities and the related exchange times strongly depend on the temperature and the number of water channels which can be blocked selectively. [53][54][55][56] The exchange time t is related to the diffusive permeability of the membrane P f = V/SÁt 71 by the volume-to-surface ratio of the cell where a maximum V/S = d/6 is reached by a sphere (d…diameter). In erythrocytes t has been determined also with cg experiments to 17 and 48 ms with open and blocked water channels, respectively.…”

Section: Size Distribution and Intracellular Diffusion Constantmentioning

confidence: 99%

Restricted diffusion and exchange of intracellular water: theoretical modelling and diffusion time dependence of1H NMR measurements on perfused glial cells

et al. 1998

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“…A Color View I CCD camera (Olympus, Japan) and a Cell^D® software were used to record minimum 15 images/sample with a 100× objective. The RBC diameters were manually measured [11, 12]. …”

Section: Methodsmentioning

confidence: 99%

“…The washing/centrifugation steps were repeated three times [11]. A quota of the resulting RBC samples were further centrifuged for 60 minutes at 50,000 g and 4°C to obtain a RBC sediment with a final hematocrit of 95% (the Carr-Purcell-Meiboom-Gill method).…”

Section: Methodsmentioning

confidence: 99%

Changes Observed in Erythrocyte Cells Exposed to an Alternating Current

Jeican

Matei

Istrate

et al. 2017

Medicine and Pharmacy Reports

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Background and aimsAppliance of electric pulses induces red blood cells (RBCs) membrane poration, membrane aminophospholipid perturbation and alteration of the normal flip-flop process, resulting in various shape changes of the RBCs. We studied morphological and water permeability changes of RBCs bombarded with electrons in an alternating current circuit.MethodsWe used three venous blood samples of 100 mL and an alternating current device. The harvested blood was divided into four experimental sets to be used for various exposure times: 0 hours (control RBCs), 0.5h, 3h and 6h (electric-stimulated RBCs).Following the electric current each of the four sets were further divided into three samples: one for the assessment of the echinocytes/RBCs ratio, another for the electron microscopy study of ultrastructural changes induced by the alternating electrical current and a larger third one for determining water permeability of RCBs by 1H-NMR spectroscopy and morphological measurements.ResultsThere is a small but statistically significant effect of the RBC exposure to alternating electric current on cell diameters. Exposure to electric current is positively and strongly correlated with the percentage of echinocytes in a duration-dependent manner. There is a strong and statistically significant correlation between electric current exposure and permeability to water as measured by 1H-NMR spectroscopy.ConclusionFollowing interactions between electric current and RBC membrane, certain modifications were observed in the erythrocyte structure. We attribute the increased cell size to a higher permeability to water and a decreased tonicity. This leads to the transformation of the RBCs into echinocytes.

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On measuring the diffusional water permeability of human red blood cells and ghosts by nuclear magnetic resonance

Cited by 60 publications

References 40 publications

Time-Dependent Diffusion MRI in Cancer: Tissue Modeling and Applications

Time-Dependent Diffusion MRI in Cancer: Tissue Modeling and Applications

Restricted diffusion and exchange of intracellular water: theoretical modelling and diffusion time dependence of1H NMR measurements on perfused glial cells

Changes Observed in Erythrocyte Cells Exposed to an Alternating Current

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