On‐stage liquid‐phase lipid microextraction coupled to nanospray mass spectrometry for detailed, nano‐scale lipid analysis

Horn, Patrick J.; Joshi, Ubisha; Behrendt, Anna K.; Chapman, Kent D.; Verbeck, Guido F.

doi:10.1002/rcm.6194

Cited by 12 publications

(4 citation statements)

References 18 publications

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“…We have developed a nanomanipulation workstation capable of collecting trace particles [10][11][12][13], single cells, and subcellular organelles [5,14,15]. The workstation can be equipped with up to four piezoelectric nanopositioners capable of simultaneously utilizing different end effectors, including microgrippers, nanospray emitters, and microelectrodes that perform low impedance electrical characterizations.…”

Section: Introductionmentioning

confidence: 99%

Nanomanipulation-Coupled Matrix-Assisted Laser Desorption/ Ionization-Direct Organelle Mass Spectrometry: A Technique for the Detailed Analysis of Single Organelles

Phelps

Chapman

Verbeck

2015

J. Am. Soc. Mass Spectrom.

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Abstract. We describe a novel technique combining precise organelle microextraction with deposition and matrix-assisted laser desorption/ionization (MALDI) for a rapid, minimally invasive mass spectrometry (MS) analysis of single organelles from living cells. A dual-positioner nanomanipulator workstation was utilized for both extraction of organelle content and precise co-deposition of analyte and matrix solution for MALDI-direct organelle mass spectrometry (DOMS) analysis. Here, the triacylglycerol (TAG) profiles of single lipid droplets from 3T3-L1 adipocytes were acquired and results validated with nanoelectrospray ionization (NSI) MS. The results demonstrate the utility of the MALDI-DOMS technique as it enabled longer mass analysis time, higher ionization efficiency, MS imaging of the co-deposited spot, and subsequent MS/MS capabilities of localized lipid content in comparison to NSI-DOMS. This method provides selective organellar resolution, which complements current biochemical analyses and prompts for subsequent subcellular studies to be performed where limited samples and analyte volume are of concern.

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Section: Introductionmentioning

confidence: 99%

Nanomanipulation-Coupled Matrix-Assisted Laser Desorption/ Ionization-Direct Organelle Mass Spectrometry: A Technique for the Detailed Analysis of Single Organelles

Phelps

Chapman

Verbeck

2015

J. Am. Soc. Mass Spectrom.

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“…This TOC cell approach has gained immense attention due to its ability to provide a unique insight into the inner workings of cancer cells [9][10][11][12]. A living organism is composed of numerous types of cells, each containing a specialized structure, function, and unique gene expression [13,14]. Therefore, two cells with an identical chemical composition will never exist; even cells of the same type/sub-type with identical or near identical genomic DNA sequences will contain unique gene expressions and thus unique metabolome profiles [13].…”

Section: Introductionmentioning

confidence: 99%

True One Cell Chemical Analysis in Cancer Research: A Review

Reyes

Verbeck

2023

J. Anal. Oncol.

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True One Cell (TOC) analysis Is becoming highly critical for functional studies of cancer cells. This is partially because it is the only form of analysis that provides an avenue for studying the heterogeneity and cell-to-cell variations of individual cancer cells, thus providing unique insight into complex regulatory processes that govern TOC functions within a tumor. Additionally, true one cell techniques are playing an increasingly important role in current attempts to implement TOC metabolomic and proteomic studies, as well as emerging attempts to spatially resolve TOC information. In this review we provide a brief overview of the basis of the field and discuss its applications in TOC metabolomics and proteomics.

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“…12 DAPNe uses a device comprised of a nanomanipulator mounted on the stage of a high-powered microscope. Nanomanipulation has been used for many forensic and biological applications including ultra-trace drug identification, 13 extraction of a peptide from an individual library of beads, 14 extraction of lipid content directly from organelle, preparations of plant tissues, 15 and ultra-trace molecular analysis. 16 The advantage of DAPNe-NSI-MS for the analyses of ink and document examination is that it keeps the document in its pristine form.…”

Section: Introductionmentioning

confidence: 99%

Investigation of falsified documents via direct analyte-probed nanoextraction coupled to nanospray mass spectrometry, fluorescence microscopy, and Raman spectroscopy

Huynh

Williams

Golden

et al. 2015

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Microscopy with direct analyte-probed nanoextraction coupled to nanospray ionization mass spectrometry (DAPNe-NSI-MS) is a direct extraction technique that extracts ultra-trace amounts of analyte. It has been proven to extract ink from documents with little to no physical or chemical footprint. In this study, DAPNe has been coupled to Raman spectroscopy, fluorescence microscopy, and NSI-MS to determine if an ink entry from a document was falsified. A handwritten number was altered using a different ink pen to test if the aforementioned techniques could discriminate the original number from the altered number, qualitatively and/or quantitatively. Chemical species from part of the original number, altered number, and a point at which both inks intersect were successfully differentiated by all techniques when using different pens. DAPNe coupled to fluorescence microscopy and Raman spectroscopy was not able to discriminate the forged ink entry when the exact same pen was used to modify the text (due to the same ink formula). However, DAPNe-NSI-MS successfully discerned that the pen was dispensed on different days by quantitating the oxidation process.

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On‐stage liquid‐phase lipid microextraction coupled to nanospray mass spectrometry for detailed, nano‐scale lipid analysis

Abstract: The modified instrumentation of a multi-port, on-stage bioworkstation shows considerable versatility by combining nanomanipulation, microextraction and direct NMS for a variety of chemical, biological, industrial, and clinical applications.

Cited by 12 publications

References 18 publications

Nanomanipulation-Coupled Matrix-Assisted Laser Desorption/ Ionization-Direct Organelle Mass Spectrometry: A Technique for the Detailed Analysis of Single Organelles

Nanomanipulation-Coupled Matrix-Assisted Laser Desorption/ Ionization-Direct Organelle Mass Spectrometry: A Technique for the Detailed Analysis of Single Organelles

True One Cell Chemical Analysis in Cancer Research: A Review

Investigation of falsified documents via direct analyte-probed nanoextraction coupled to nanospray mass spectrometry, fluorescence microscopy, and Raman spectroscopy

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