1989
DOI: 10.1002/bit.260340217
|View full text |Cite
|
Sign up to set email alerts
|

On the effectiveness of selection pressure through use of a complementing product

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

1992
1992
2024
2024

Publication Types

Select...
2
1

Relationship

0
3

Authors

Journals

citations
Cited by 3 publications
(1 citation statement)
references
References 6 publications
0
1
0
Order By: Relevance
“…Nevertheless, the underlying biology of such phenomenon is still a subject of debate, with three major hypotheses proposed. Firstly, by placing the auxotrophic selection marker gene on a plasmid, the plasmid-carrying cells could potentially over-synthesise the complementary metabolite(s) (CMs), wherein the residual intracellular excess could be inherited by plasmid-free daughter cells and allow them to undergo several divisions within a selective environment after the mitotic event 33 37 . However, when Shou and colleagues cultivated lys2∆ and ade8∆ S. cerevisiae strains firstly as monocultures in synthetic complete (SC) media and subsequently co-cultivated them into SC-Lys-Ade media, they reported both strains quickly died after an initial, transient growth period of ~10 h using the stored metabolites 22 .…”
Section: Resultsmentioning
confidence: 99%
“…Nevertheless, the underlying biology of such phenomenon is still a subject of debate, with three major hypotheses proposed. Firstly, by placing the auxotrophic selection marker gene on a plasmid, the plasmid-carrying cells could potentially over-synthesise the complementary metabolite(s) (CMs), wherein the residual intracellular excess could be inherited by plasmid-free daughter cells and allow them to undergo several divisions within a selective environment after the mitotic event 33 37 . However, when Shou and colleagues cultivated lys2∆ and ade8∆ S. cerevisiae strains firstly as monocultures in synthetic complete (SC) media and subsequently co-cultivated them into SC-Lys-Ade media, they reported both strains quickly died after an initial, transient growth period of ~10 h using the stored metabolites 22 .…”
Section: Resultsmentioning
confidence: 99%