On the factors affecting the enhanced resonance light scattering signals of the interactions between proteins and multiply negatively charged chromophores using water blue as an example

Huang, Cheng; Lu, Wei; Li, Yuan Fang; Huang, Yu

doi:10.1016/j.aca.2005.09.048

Cited by 36 publications

(15 citation statements)

References 24 publications

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“…Although the plasma samples deproteinated by methanol and ethanol were 25 times more dilute than the samples treated by either acetone or acetonitrile, the albumin peaks were still larger in the case of both methanol and ethanol. Albumin is the main protein in the plasma [30] and it is clear from the electropherograms in Fig. 1 that acetonitrile and acetone are much stronger deproteinisers than methanol and ethanol.…”

Section: Protein Determinationmentioning

confidence: 96%

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Comparison of different water-miscible solvents for the preparation of plasma and urine samples in metabolic profiling studies

Alzweiri

Watson

Robertson

et al. 2008

Talanta

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This version is available at https://strathprints.strath.ac.uk/7602/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the AbstractThe Lowry method and a capillary electrophoresis method were used to analyse protein residues in the supernatant after solvent deproteination of plasma. Acetonitrile and acetone were much more effective than methanol and ethanol at reducing the levels of proteins in plasma. The ability of different solvents to decrease levels of phospholipids in plasma samples was assessed using electrospray ionisation mass spectrometry (MS). Phospholipid signals can obscure differences between samples in general metabolite profiling (i.e. non-target compound) studies. Acetonitrile was much more effective than methanol in reducing the MS signal due to phospholipids in plasma which is a consequence of the poor solubility of phospholipids in acetonitrile. The capability of the solvents at reducing salts in urine samples was also studied by using an amperometric method. Using this approach little difference was detected between methanol, ethanol, acetonitrile and acetone in their ability to desalt urine samples.

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Section: Protein Determinationmentioning

confidence: 96%

“…In order to accomplish this, polybrene, which is a pH-independent cationic polymer, was used to coat the inner wall of the silica capillary, and the pH of the analysis buffer ensured that albumin, which has an isoelectric point of 4.9 [30], was positively charged during the analysis. Fig.…”

Section: Protein Determinationmentioning

confidence: 99%

Comparison of different water-miscible solvents for the preparation of plasma and urine samples in metabolic profiling studies

Alzweiri

Watson

Robertson

et al. 2008

Talanta

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“…As an analytical technique, RLS is promising and attractive due to its distinct advantages of high sensitivity, convenient operation, simplicity and inexpensive reagents and speediness. Since Pasternack 1,2 first detected assemblies of porphyrin on DNA by the RLS technique, this technique can be used for the quantification of nucleic acids, [3][4][5][6] proteins, 7 pharmaceutical drugs, 8 metal ions, 9 surfactants, 10 sugars, 11 and so on. There is substantial and growing interest in using conjugated polymers (CPs) as the responsive basis for chemical and biological detection schemes.…”

Section: Introductionmentioning

confidence: 99%

Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

Zheng

Huang

et al. 2009

ANAL. SCI.

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Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (DIRLS) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 mg mL -1 , and the detection limit (S/N = 3) was 17.0 ng mL -1 . The binding ratio and the binding constant between DNA and polymer were also provided.

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“…Pasternack et al first established the RLS technique to study the biological macromolecules by means of an ordinary fluorescence spectrometer [3][4][5]. Due to their high sensitivity, selectivity, and convenience, RLS studies have attracted great interest among researchers [6][7][8][9][10]. RLS has emerged as a very attractive technique that has been used to monitor molecular assemblies and characterize the extended aggregates of chromophores.…”

Section: Introductionmentioning

confidence: 99%

The Degradation of Organic Dyes by Corona Discharge

Goheen¹,

McCulloch²,

E³

et al. 2016

Dyes and Drugs

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A resonance light scattering technique to determine tinidazole in drugs was developed by tetraphenylboron sodium (TPB). Tinidazole was found to bind B(C 6 H 5 ) − 4 anion and transformed to tinidazole-TPB aggregate which displayed intense resonance scattering light. Effects of factors such as wavelength, acidity, stabilizers, and interferents on the RLS of tinidazole TPB were investigated in detail. The RLS intensity of the tinidazole-TPB suspension was obtained in sulfuric acid solution (pH = 1.44). The resonance scattering light intensity at the maximum RLS peak of 569.5 nm was linear to the concentration of tinidazole in the range of 10.0-30.0 μg mL −1 with a detection limit of 5.0 μg mL −1 . Good results were also obtained with the recovery range of 95.13-106.76%. The method was applied to determine tinidazole in injections and tablets, showing high sensitivity and accuracy compared with the high performance liquid chromatography method (HPLC) according to Chinese Pharmacopoeia.

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On the factors affecting the enhanced resonance light scattering signals of the interactions between proteins and multiply negatively charged chromophores using water blue as an example

Cited by 36 publications

References 24 publications

Comparison of different water-miscible solvents for the preparation of plasma and urine samples in metabolic profiling studies

Comparison of different water-miscible solvents for the preparation of plasma and urine samples in metabolic profiling studies

Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

The Degradation of Organic Dyes by Corona Discharge

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