The fossorial snake Apostolepis kikoi Santos et al., 2018 is known only from its type locality, in Chapada dos Guimarães, state of Mato Grosso, Brazil. Here, we present the first records of this species after its description, expanding its distribution to transition areas between the Amazon and Cerrado biomes, in the southwestern portion of Mato Grosso. The new record expands the known distribution of A. kikoi by 297 km west from the type locality. In addition, we provide data on morphological variation by comparing our sample with the type series.