On the question of vegetative reproduction in apiculate yeasts

Structural Changes in the Cell Wall ofSchizosaccharomyces pombeDuring Cell Division

“…budding yeast cells fluoresces more intensively (25). Electron micrographs exhibit a distinct margin of the scar both in the metallized walls ( Fig.…”

“…Culture and cultivation. S. pombe 221 from the National Collection of the Czechoslovak Academy of Sciences was grown in the synthetic medium of Olson and Johnson (20) containing: glucose, 10 g; NH4H2PO4, 6 g; KH2PO4, 0.2 g; MgSO4-7H20, 0.25 g; sodium citrate, 1 g; L-asparagine, 2.5 g; biotin, 20 ,ug; calcium pantothenate, 0.5 mg; inositol, 10 mg; thiamine, 4 mg; pyridoxine, 1 mg; zinc (as sulfate), 400 jug; iron (as ferrous ammonium sulfate), 150 jug; copper (as sulfate), 25 jig; and distilled water to 1 liter. The pH was adjusted to 5.0 with phosphoric acid.…”

“…It takes up the entire cell apex of a cylindrical cell and is slightly convex because of turgor. Like the plug of a bud scar and of a multiple scar (25), it shows negligible fluorescence (Fig. 3d, sp).…”

Multiple Scars, a New Type of Yeast Scar in Apiculate Yeasts

“…No similar structures were found on extensive experimental material. The present knowledge of multiple scars has become the basis for interpreting the vegetative reproduction of apiculate yeasts (Streiblovi and Beran, 1965). In addition, it simplifies the study of the architectonics of multiple scars in ultrathin sections and the solving of the question of their chemical composition and structure.…”

A study of the budding ofSaccharomyces uvarum Beijerinck with the scanning electron microscope

“…These observations were confirmed with old cells on which the scars were either mainly at the pole, or over the whole surface (Streiblovfi, 1970). The first buds appeared neither at random (Bartholomew and Mittwer, 1953), nor according to the process described by Streiblov~ and Beran (1965).…”