On the red fluorescence emission of &amp;lt;i&amp;gt;Aggregatibacter actinomycetemcomitans&amp;lt;/i&amp;gt;

Bjurshammar, Nadja; Johannsen, Annsofi; Buhlin, Kåre; Tranæus, Sofia; Östman, Conny

doi:10.4236/ojst.2012.24052

Cited by 12 publications

(6 citation statements)

References 20 publications

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“…The fluorescence may be the result of the production of metabolic by-products from microorganisms in the plaque ( 11 ). Since not all dental plaque fluoresces red, it has been suggested that it is the mature dental plaque that fluoresces red ( 7 , 12 , 13 ) and that it is related to its cariogenicity as well ( 14 ). Active caries lesions as well as dental plaque have been shown to fluoresce red, when excited with violet light ( 11 , 15 – 17 ), which is in line with the hypothesis that red fluorescence is related to the cariogenicity of plaque.…”

mentioning

confidence: 99%

Red fluorescent biofilm: the thick, the old, and the cariogenic

Volgenant¹,

Hoogenkamp²,

Buijs³

et al. 2016

Journal of Oral Microbiology

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BackgroundSome dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown.ObjectiveThe aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms.DesignThe effects of biofilm age and thickness on red fluorescence were tested in a constant depth film fermentor (CDFF) by growing biofilms of variable thicknesses that received a constant supply of defined mucin medium (DMM) and eight pulses of sucrose/day. The influence of cariogenicity on red fluorescence was tested by growing biofilm on dentin disks receiving DMM, supplemented with three or eight pulses of sucrose/day. The biofilms were analyzed at different time points after inoculation, up to 24 days. Emission spectra were measured using a fluorescence spectrophotometer (λexc405 nm) and the biofilms were photographed with a fluorescence camera. The composition of the biofilms was assessed using 454-pyrosequecing of the 16S rDNA gene.ResultsFrom day 7 onward, the biofilms emitted increasing intensities of red fluorescence as evidenced by the combined red fluorescence peaks. The red fluorescence intensity correlated with biofilm thickness but not in a linear way. Biofilm fluorescence also correlated with the imposed cariogenicity, evidenced by the induced dentin mineral loss. Increasing the biofilm age or increasing the sucrose pulsing frequency led to a shift in the microbial composition. These shifts in composition were accompanied by an increase in red fluorescence.ConclusionsThe current study shows that a thicker, older, or more cariogenic biofilm results in a higher intensity of red fluorescence.

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confidence: 99%

Red fluorescent biofilm: the thick, the old, and the cariogenic

Volgenant¹,

Hoogenkamp²,

Buijs³

et al. 2016

Journal of Oral Microbiology

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“…(8,11,15,(19)(20)(21) In addition, Han et al, (21) emphasized the correlation between reddish fluorescence and presence of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum, which are pathogenic anaerobic bacteria present in mature biofilm and related to periodontal disease. (21) Bjurshammar et al, (29) showed that Aggregatibacter actinomycetemcomitans is capable of emitting reddish fluorescence and that there is correlation between colony growth time and increased reddish fluorescence intensity. (29) Thus, these previous studies validate the use presented in this case for the WOF, to visualize mature biofilm of greater pathogenic potential, allowing its complete removal.…”

Section: ❚ Discussionmentioning

confidence: 99%

“…(21) Bjurshammar et al, (29) showed that Aggregatibacter actinomycetemcomitans is capable of emitting reddish fluorescence and that there is correlation between colony growth time and increased reddish fluorescence intensity. (29) Thus, these previous studies validate the use presented in this case for the WOF, to visualize mature biofilm of greater pathogenic potential, allowing its complete removal. Furthermore, report of the patient about the progression of 3 months for perception of the signs of peri-implant mucositis is more than enough to establish a mature and pathogenic biofilm.…”

Section: ❚ Discussionmentioning

confidence: 99%

Use of wide-field optical fluorescence for visualization of oral biofilm in a patient with peri-implant mucositis: a new approach

Andrade

Pratavieira

Varotti

2021

Einstein (São Paulo)

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Peri-implant diseases, caused by bacteria from biofilm related to dental implants, are one of the main causes of late loss of implants. In this sense, peri-implant diseases are divided into periimplant mucositis, when it affects only the soft tissues, and peri-implantitis, when there is a bone involvement, which can lead to the failure of dental implant therapy. Thus, biofilm removal is essential for peri-implant health, allowing long-term success in implant therapy. To improve the visualization of oral biofilm, which is usually transparent or colorless, disclosing agents have been routinely used. However, disclosing agents have allergenic potential and can cause staining extrinsically in restorative and prosthetic materials, leading to aesthetic impairment. Thus, the use of fluorescence has been studied as an alternative for visualization of oral biofilm. Therefore, this report describes the use of wide-field optical fluorescence for visualization of oral biofilm associated with implants and teeth, in a routine appointment and follow-up of a partially edentulous patient with peri-implant mucositis. In addition, this report showed wide-field optical fluorescence can be used in a clinical routine of care of patients with dental implants. In this sense, wide-field optical fluorescence allowed easy and immediate visualization of the mature oral biofilm for its adequate removal, evaluation of the quality of restoration to sealing of screw access-hole of implant and identification of cariogenic lesions, without risk of allergic reactions or staining of prostheses and restorations.

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“…While interpreting the results of this study, it should be also be understood that variations in the microbiological compositions of plaque, 44 pigmentation, and the use of some toothpastes or prophylaxis pastes 45 can also cause variations in spectral intensity values. The merits of this study include a sufficiently large sample size, stringent selection criteria, and the use of a nonionizing 404 nm wavelength for excitation.…”

Section: Limitations and Strengthsmentioning

confidence: 99%

Detection and quantification of dental plaque based on laser-induced autofluorescence intensity ratio values

Joseph¹,

Prasanth

Jayanthi

et al. 2015

J. Biomed. Opt

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The aim of this study was to evaluate the applicability of laser-induced autofluorescence (LIAF) spectroscopy to detect and quantify dental plaque. LIAF spectra were recorded in situ from dental plaque (0–3 grades of plaque index) in 300 patients with 404 nm diode laser excitation. The fluorescence intensity ratio of the emission peaks was calculated from the LIAF spectral data following which their scatter plots were drawn and the area under the receiver operating characteristics were calculated. The LIAF spectrum of clinically invisible grade-1 plaque showed a prominent emission peak at 510 nm with a satellite peak around 630 nm in contrast to grade 0 that has a single peak around 500 nm. The fluorescence intensity ratio (F510/F630) has a decreasing trend with increase in plaque grade and the ratio values show statistically significant differences (p<0.01) between different grades. An overall sensitivity and specificity of 100% each was achieved for discrimination between grade-0 and grade-1 plaque. The clinical significance of this study is that the diagnostic algorithm developed based on fluorescence spectral intensity ratio (F510/F630) would be useful to precisely identify minute amounts of plaque without the need for disclosing solutions and to convince patients of the need for proper oral hygiene and homecare practices.

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On the red fluorescence emission of <i>Aggregatibacter actinomycetemcomitans</i>

Cited by 12 publications

References 20 publications

Red fluorescent biofilm: the thick, the old, and the cariogenic

Red fluorescent biofilm: the thick, the old, and the cariogenic

Use of wide-field optical fluorescence for visualization of oral biofilm in a patient with peri-implant mucositis: a new approach

Detection and quantification of dental plaque based on laser-induced autofluorescence intensity ratio values

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