On the Structural Stability and Solvent Denaturation of Proteins

Herskovits, Theodore T.; Jaillet, Helen; Gadegbeku, Barbara

doi:10.1016/s0021-9258(18)62563-3

Cited by 79 publications

(75 citation statements)

References 25 publications

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“…Pantoja-Castro and González-Rodríguez (2011) reported that the hydrolysable tannin, which provides a high dye affinity, is extracted better in distilled water than alcohol. Moreover, the dyes extracted by alcohol generally cause the denaturation of the protein structures in the leather (Herskovits et al , 1970). Thus, in spite of increasing the dye concentration, the dye molecules were not dispersed into the protein structure.…”

Section: Resultsmentioning

confidence: 99%

Improvement of dye affinity in natural dyeing usingTerminalia chebula retzius(T. chebula) applied to leather

Song

Kim

2017

IJCST

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Purpose The purpose of this paper is to improve the dye affinity of natural dye of Terminalia chebula retzius (T. chebula) using the dye substrate of leather. Design/methodology/approach The dyeing conditions such as temperature, concentration of dye, and time are controlled by measuring the dye affinity. The effect of enzymatic post-tanning process on dye affinity is evaluated by using different type of proteases such as flavourzyme, alcalase, and bromelain. The optimum conditions for enzymatic post-tanning process are evaluated depending on different pH, temperature, and concentration of enzyme. Findings The highest dye affinity was obtained at 50°C using a dye concentration of 200 percent (owf) for 30 min treatment by measuring of K/S values of dyed leather. Distilled water was proved as a better extraction liquid to improve the dye affinity of T. chebula. The K/S values of dyed leather were enhanced after the enzymatic post-tanning process by flavourzyme. Moreover, the fastness properties against the rubbing and dry cleaning of the dyed leather were improved by the enzymatic post-tanning process. Originality/value In this paper, the enzymatic post-tanning process is introduced as the method to improve the dye affinity in natural dyeing using Terminalia chebula retzius (T. chebula) applied to leather. The results of the study could be applied for further natural dyeing of leather using various natural dyes.

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Section: Resultsmentioning

confidence: 99%

Improvement of dye affinity in natural dyeing usingTerminalia chebula retzius(T. chebula) applied to leather

Song

Kim

2017

IJCST

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“…Moreover, DLc1 displayed extraordinary stability under vigorous mechanical disturbance (vortex), possibly due to the excellent stiffness of its capsid, as demonstrated in phage ϕ29 [73]. The declined infectivity under shaking in the existence of chloroform could be attributed to the disruption of hydrophobic interactions in amino acids, resulting in the destabilization of proteins [74,75].…”

Section: The Application Potentialmentioning

confidence: 97%

“…For the pH stability test, the pH value of ultrapure water was adjusted to 1, 3, 5, 7, 9, 11, and 13, respectively, using 0.1 M HCl and 0.1 M NaOH solution. The phage was suspended in the above solution to a final titer of 1 × 10 8 PFU/mL and kept in dark at 25 • C for 1 h. Stability of DLc1 in NaCl and ethanol solution was measured by suspending phage in NaCl solution with different concentrations (50,100,200, 300, 400, 500, and 1000 mM) and different ethanol solutions (10,25,50,75, and 90%) to a final titer of 1 × 10 8 PFU/mL and kept at 4 • C for 1 h.…”

Section: Stability Of Dlc1mentioning

confidence: 99%

Isolation and Characterization of Bacillus cereus Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages

Yuan

et al. 2020

Microorganisms

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Bacillus phage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage, named Bacillus cereus phage vB_BceP-DLc1. This phage, with a unique inserted gene cluster, has the largest genome among known φ29-like phages. DLc1 can use the surface carbohydrate structures of the host cell as receptors and only infects the most related B. cereus strains, showing high host-specificity. The adsorption rate constant and life cycle of DLc1 under experimental conditions were also determined. Not only stable under temperatures below 55 °C and pH range from 5 to 11, the new phage also showed tolerance to high concentrations of NaCl, 75% ethanol, chloroform, and mechanical vortex, which is preferable for practical use in the food and pharmaceutical industries.

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“…Παρόλα αυτά ο πρώτος ερευνητής που κατάφερε να σχεδιάσει και να αναπαραγάγει τις μεθόδους κρυστάλλωσης της αιμοσφαιρίνης ήταν ο Funke το 1851 οπότε και προβλέφθηκε οτι η μελέτη των κρυστάλλων θα αποτελούσε στο μέλλον το πιο σημαντικό εργαλείο για τη διερεύνηση της φύσης των βιολογικών μακρομορίων (Reichert & Brown, 1909). Στη συνέχεια ακολούθησε η κρυστάλλωση και άλλων πρωτεϊνών όπως της σλβουμίνης του αβγού και διαφόρων φυτικών πρωτεϊνών (Hofmeister, 1888), της ουρεάσης από φασόλια (Sumner, 1926), της πεψίνης και άλλων πρωτεολυτικών ενζύμων (Northrop ei a/., 1948) (Blundell & Johnson, 1976;Drenth, 1999 (Schrier & Scheraga, 1962;Herskovits et al, 1970;Rhodes, 2000). Αυτό καθιστά την επιλογή ενός οργανικού διαλύτη ως παράγοντα καθίζησης, κρίσιμη και περιορισμένη σε αριθμό.…”

Section: /'' ^1 Wlf-ί-wunclassified

Σχεδιασμός Αναστολέων Της Γλυκογονικής Φωσφορυλάσης Ως Εν Δυνάμει Αντιδιαβητικά Φάρμακα

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On the Structural Stability and Solvent Denaturation of Proteins

Cited by 79 publications

References 25 publications

Improvement of dye affinity in natural dyeing usingTerminalia chebula retzius(T. chebula) applied to leather

Improvement of dye affinity in natural dyeing usingTerminalia chebula retzius(T. chebula) applied to leather

Isolation and Characterization of Bacillus cereus Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages

Σχεδιασμός Αναστολέων Της Γλυκογονικής Φωσφορυλάσης Ως Εν Δυνάμει Αντιδιαβητικά Φάρμακα

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