On Tyrosine and Tryptophane Determinations in Proteins

Folin, Otto; Ciocalteu, Vintila

doi:10.1016/s0021-9258(18)84277-6

Cited by 2,481 publications

(346 citation statements)

References 4 publications

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“…To determine the polyphenols, the spectrophotometry method with Folin-Ciocâlteu reagent [49][50][51] was used and the reagent consisted of an aqueous solution of phosphomolybdate and phosphotungstate. For these analyses, the filtrate obtained previously was diluted at 1:10.…”

Section: Determination Of Polyphenols By the Folin-ciocâlteu Methodsmentioning

confidence: 99%

Comparison of Bioactive Substances Content between Commercial and Wild-Type Isolates of Pleurotus eryngii

et al. 2021

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Mushrooms belonging to Pleurotus genus have been demonstrated to have important nutritional and medicinal value and their regular intake prevent many diseases, reduce the infection probability and increase immunity. In order to investigate the bioactive compounds produced by seven commercial (‘142 F’, ‘142 E’, ‘D+’, ‘V turbo’, ‘V 142’, ‘A 12’, ‘V 160’) and five wild-type (‘Albino 1107’, ‘Altamura 1603’, ‘Muro Lucano 139’, ‘Conversano 1250’, ‘Albino beige chiaro 1094’) P. eryngii isolates, the following qualitative analyses were performed: Total polyphenol content, antioxidant activity (EC50 of ABTS) and antiradical power (ARP) in fresh lyophilized and dry basidioma, and water content, β-glucans and phenolic compounds in fresh samples. Standard methods were employed for each of the above mentioned aims. Total polyphenol content was diverse among the P. eryngii isolates. In particular, an elevated polyphenolic content was found in fresh lyophilized P. eryngii samples of the commercial isolates ‘V 142’ followed by ‘A 12’. The highest antiradical activity (ARP) was obtained by ‘Muro Lucano 139’ isolate. Wild P. eryngii isolates were characterized by higher water and β-glucans contents compared to the commercial ones, and the highest values were registered for the ‘Albino beige chiaro 1094’ isolate. In conclusion, the present study allowed us to identify the commercial and wild-type P. eryngii isolates from the Basilicata region, with high nutritional and medicinal value based on their bioactive compounds.

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Section: Determination Of Polyphenols By the Folin-ciocâlteu Methodsmentioning

confidence: 99%

Comparison of Bioactive Substances Content between Commercial and Wild-Type Isolates of Pleurotus eryngii

et al. 2021

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“…The proteolytic activity of immobilized proteases was evaluated using the Anson's and Folin and Ciocalteu's methods [37,38] employing casein as substrate. A 0.65% weight/ volume casein solution, prepared by dissolving 6.5 mg per each mL of a 50 mM potassium phosphate buffer at pH 8.6, was used.…”

Section: Enzymatic Testsmentioning

confidence: 99%

Covalent Immobilization of Proteases on Polylactic Acid for Proteins Hydrolysis and Waste Biomass Protein Content Valorization

et al. 2021

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The recovery of the protein component and its transformation into protein hydrolysates, generally carried out chemically, gives great added value to waste biomasses. The production of protein hydrolysates through enzymatic catalysis would guarantee to lower the environmental impact of the process and raise product quality, due to the reproducible formation of low molecular weight peptides, with interesting and often unexplored biological activities. The immobilization of the enzymes represents a good choice in terms of stability, recyclability and reduction of costs. In this context, we covalently linked proteases from Aspergillus oryzae to polylactic acid an eco-friendly biopolymer. The hydrolytic efficiency of immobilized enzymes was assessed testing their stability to temperature and over time, and checking the hydrolysis of model biomasses (casein and bovine serum albumin). Soybean waste extracts were also used as proof of principle.

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“…Powdered wheat tissue was homogenized on ice with 1 mL of 80% ethanol (1:10, w/v) and phenolic compounds were extracted for 24 h at −20 • C. After extraction, samples were centrifuged at 21,000× g at 4 • C for 15 min and supernatant was used for further measurements. Soluble phenolic content was determined by the Folin-Ciocalteu method [42]. The reaction mixture contained 20 µL of sample, 1.58 mL of H 2 O, 100 µL of Folin-Ciocalteu reagent, and 300 µL of the saturated Na 2 CO 3 solution.…”

Section: Determination Of the Soluble Phenolic Contentmentioning

confidence: 99%

Oxidative Status and Antioxidative Response to Fusarium Attack and Different Nitrogen Levels in Winter Wheat Varieties

Matić

Vuković

Vrandečić

et al. 2021

Plants

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Abiotic and biotic stresses, such as mineral nutrition deficiency (especially nitrogen) and Fusarium attack, pose a global threat with devastating impact on wheat yield and quality losses worldwide. This preliminary study aimed to determine the effect of Fusarium inoculation and two different nitrogen levels on oxidative status and antioxidative response in nine wheat varieties. Level of lipid peroxidation, activities of antioxidant enzymes (catalase, ascorbate peroxidase, glutathione reductase), phenolics, and chloroplast pigments content were measured. In general, wheat variety, nitrogen, and Fusarium treatment had an impact on all tested parameters. The most significant effect had a low nitrogen level itself, which mostly decreased activities of all antioxidant enzymes and reduced the chloroplast pigment content. At low nitrogen level, Fusarium treatment increased activities of some antioxidative enzymes, while in a condition of high nitrogen levels, antioxidative enzyme activities were mostly decreased due to Fusarium treatment. The obtained results provided a better understanding on wheat defense mechanisms against F. culmorum, under different nitrogen treatments and can serve as an additional tool in assessing wheat tolerance to various environmental stress conditions.

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On Tyrosine and Tryptophane Determinations in Proteins

Cited by 2,481 publications

References 4 publications

Comparison of Bioactive Substances Content between Commercial and Wild-Type Isolates of Pleurotus eryngii

Comparison of Bioactive Substances Content between Commercial and Wild-Type Isolates of Pleurotus eryngii

Covalent Immobilization of Proteases on Polylactic Acid for Proteins Hydrolysis and Waste Biomass Protein Content Valorization

Oxidative Status and Antioxidative Response to Fusarium Attack and Different Nitrogen Levels in Winter Wheat Varieties

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