Oncogenic miRNAs Identified in Tear Exosomes From Metastatic Breast Cancer Patients

Inubushi, Sachiko; Kawaguchi, Hiroki; Mizumoto, Sachiko; Kunihisa, Tomonari; Baba, Motoi; Kitayama, Yukiya; Takeuchi, Toshifumi; Hoffman, Robert M.; Sasaki, Ryohei

doi:10.21873/anticanres.14290

Cited by 68 publications

(59 citation statements)

References 11 publications

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“…Inubushi et al, who have revealed significantly higher quantity of exosome markers in tears than in serum, have recently confirmed the importance of tear fluid, especially tear exosomes, in breast cancer management. Moreover, miRNAs (miRNA-21 and miRNA-200c) specific to breast cancer were highly expressed in tear exosomes of metastatic breast cancer patients when compared to healthy control [ 195 ]. In addition to tear fluid, molecular analysis of breast milk could lead to the identification of proteins valuable for early detection and accurate assessment of breast cancer [ 151 ].…”

Section: Other Liquid Biopsy Typesmentioning

confidence: 99%

Liquid Biopsy is Instrumental for 3PM Dimensional Solutions in Cancer Management

Líšková

Samec

Koklesová

et al. 2020

JCM

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One in every four deaths is due to cancer in Europe. In view of its increasing incidence, cancer became the leading cause of death and disease burden in Denmark, France, the Netherlands, and the UK. Without essential improvements in cancer prevention, an additional 775,000 cases of annual incidence have been prognosed until 2040. Between 1995 and 2018, the direct costs of cancer doubled from EUR 52 billion to EUR 103 billion in Europe, and per capita health spending on cancer increased by 86% from EUR 105 to EUR 195 in general, whereby Austria, Germany, Switzerland, Benelux, and France spend the most on cancer care compared to other European countries. In view of the consequent severe socio-economic burden on society, the paradigm change from a reactive to a predictive, preventive, and personalized medical approach in the overall cancer management is essential. Concepts of predictive, preventive, and personalized medicine (3PM) demonstrate a great potential to revise the above presented trends and to implement cost-effective healthcare that benefits the patient and society as a whole. At any stage, application of early and predictive diagnostics, targeted prevention, and personalization of medical services are basic pillars making 3PM particularly attractive for the patients as well as ethical and cost-effective healthcare. Optimal 3PM approach requires novel instruments such as well-designed liquid biopsy application. This review article highlights current achievements and details liquid biopsy approaches specifically in cancer management. 3PM-relevant expert recommendations are provided.

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Section: Other Liquid Biopsy Typesmentioning

confidence: 99%

Liquid Biopsy is Instrumental for 3PM Dimensional Solutions in Cancer Management

Líšková

Samec

Koklesová

et al. 2020

JCM

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“…Exosomes are found in tears in normal as well as cancer patients. Cancer-specific markers are highly expressed in a study conducted in metastatic breast cancer patients [ 31 ]. Sjogren’s syndrome (SS) is an autoimmune disease that targets glandular tissues, including the lacrimal glands and exocrine salivary glands, where local inflammation is thought to manifest primary symptoms, such as xerostomia and xerophthalmia.…”

Section: Exosomes Of the Eyementioning

confidence: 99%

“…The presence of T cell exosome-derived miR-142-3p in SS suggests it as a potential driver in the immunopathology [ 32 ]; also, exosomes are already proven to transfer miRNA between cells [ 33 ], and it has a functional role in the glandular cell dysfunction [ 32 ]. Breast cancer-specific miR-21 and miR-200c show higher expression levels in tear exosomes of metastatic breast cancer patients as opposed to healthy controls [ 31 ]. Most of the studies suggest exosomal constituents of tears to be biomarkers.…”

Section: Exosomes Of the Eyementioning

confidence: 99%

Exosomes: Insights from Retinoblastoma and Other Eye Cancers

Lande

Gupta

Ranjan

et al. 2020

IJMS

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Exosomes, considered as cell debris or garbage bags, have been later characterized as nanometer-sized extracellular double-membrane lipid bilayer bio-vesicles secreted by the fusion of vesicular bodies with the plasma membrane. The constituents and the rate of exosomes formation differ in different pathophysiological conditions. Exosomes are also observed and studied in different parts of the eye, like the retina, cornea, aqueous, and vitreous humor. Tear fluid consists of exosomes that are shown to regulate various cellular processes. The role of exosomes in eye cancers, especially retinoblastoma (RB), is not well explored, although few studies point towards their presence. Retinoblastoma is an intraocular tumor that constitutes 3% of cases of cancer in children. Diagnosis of RB may require invasive procedures, which might lead to the spread of the disease to other parts. Due to this reason, better ways of diagnosis are being explored. Studies on the exosomes in RB tumors and serum might help designing better diagnostic approaches for RB. In this article, we reviewed studies on exosomes in the eye, with a special emphasis on RB. We also reviewed miRNAs expressed in RB tumor, serum, and cell lines and analyzed the targets of these miRNAs from the proteins identified in the RB tumor exosomes. hsa-miR-494 and hsa-miR-9, upregulated and downregulated, respectively in RB, have the maximum number of targets. Although oppositely regulated, they share the same targets in the proteins identified in RB tumor exosomes. Overall this review provides the up-to-date progress in the area of eye exosome research, with an emphasis on RB.

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“…3 Exosomes were found in a variety of body fluids, such as plasma, serum, urea, saliva and tears. 4 Exosomes act on receptor cells in three steps: (1) the transmembrane proteins of the exosomal bodies directly interact with the signal receptors of the target cells, (2) the plasma membranes of the exosomes fuse with the receptor cells and transfer their contents to the cytoplasm, and finally (3) the exosomes are directly endocytosed by the receptor cells or ingested by the receptor cells through phagocytosis. 5,6 In recent years, exosomes gained increasing attention as an important biomarker of many diseases and a medium of intercellular communication in the progression, invasion, metastasis and recurrence of many cancers.…”

Section: Introductionmentioning

confidence: 99%

“…5,6 In recent years, exosomes gained increasing attention as an important biomarker of many diseases and a medium of intercellular communication in the progression, invasion, metastasis and recurrence of many cancers. 4 Several features of exosomes have emerged, especially in cancer biology. First, exosomes contain a various biological information and functions.…”

Section: Introductionmentioning

confidence: 99%