One Health Approach Reveals the Absence of Methicillin-Resistant Staphylococcus aureus in Autochthonous Cattle and Their Environments

Correia, Susana; Silva, Vanessa; García‐Díez, Juan; Teixeira, Paula; Pimenta, Kevin; Pereira, José Eduardo; Oliveira, Soraia; Rocha, Jaqueline; Manaia, Célia M.; Igrejas, Gilberto; Poeta, Patrícia

doi:10.3389/fmicb.2019.02735

Cited by 14 publications

(15 citation statements)

References 18 publications

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“…However, contamination routes within pig production environments still remain obscure considering that clones classified as community-acquired MRSA (CA-MRSA), such as ST1/t127 and ST30/t318, were also found among the MRSA isolated ( Table 3 ), which is in agreement with previously reported data [ 64 ]. Regarding ST30, is important to remark that despite being commonly categorised as methicillin-sensitive S. aureus (MSSA) [ 65 ], MRSA variants have been isolated from pigs in Australia [ 66 ] and Portugal [ 67 ] that indicate not only a worldwide dissemination of this specific ST among pig herds, but tangible evidence of the dissemination of methicillin resistance via integration of the staphylococcal cassette chromosome mec (SCC mec ) among sensitive strains [ 68 ]. Additionally, ST30 showed a remarkably high level of MDR ( Table S2 ), harboured several antibiotic resistance and enterotoxin genes, and was identified as a strong biofilm producer ( Table 2 ), which is highly relevant in the food production chain due to its potential harmfulness and environmental recalcitrance.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Rodríguez-López

Filipello

Ciccio

et al. 2020

Foods

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The main aim of the present study was to evaluate the level of antibiotic resistance, prevalence and virulence features of methicillin-resistant Staphylococcus aureus (MRSA) isolated from heavy swine at abattoir level and farming environments in Lombardy (Northern Italy). With this scope, 88 different heavy swine farms were surveyed, obtaining a total of n = 440 animal swabs and n = 150 environmental swabs. A total of n = 87 MRSA isolates were obtained, with an overall MRSA incidence of 17.50% (n = 77) among animal samples and a 6.67% (n = 10) among environmental. Molecular characterisation using multilocus sequence typing (MLST) plus spa-typing showed that sequence type ST398/t899 and ST398/t011 were the most commonly isolated genotypes, although other relevant sequence types such as ST1 or ST97 were also found. A lack of susceptibility to penicillins, tetracycline and ceftiofur was detected in >91.95, 85.05 and 48.28% of the isolates, respectively. Resistance to doxycycline (32.18%), enrofloxacin (27.59%) and gentamicin (25.29%) was also observed. Additionally, a remarkable level of antibiotic multiresistance (AMR) was observed representing a 77.01% (n = 67) of the obtained isolates. Genetic analysis revealed that 97.70% and 77.01% of the isolates harboured at least one antibiotic resistance or enterotoxin gene, respectively, pointing out a high isolate virulence potential. Lastly, 55.17% (n = 48) were able to produce measurable amounts of biofilm after 24 h. In spite of the current programmes for antibiotic reduction in intensively farming, a still on-going high level of AMR and virulence potential in MRSA was demonstrated, making this pathogen a serious risk in swine production chain, highlighting once more the need to develop efficient, pathogen-specific control strategies.

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Section: Discussionmentioning

confidence: 99%

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Rodríguez-López

Filipello

Ciccio

et al. 2020

Foods

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“…In fact, studies have shown that CC97 subclades for human infection originated in bovine-to-human host dissemination, which indicates that animals may act as S. aureus reservoirs that can spread to humans [ 27 , 30 ]. The autochthonous Maronesa cattle is a traditional Portuguese breed used for meat production commercialized with PDO—Protected Designation of Origin [ 31 , 32 ]. Maronesa cattle is considered a threatened breed that has been used for centuries in agricultural work [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…The autochthonous Maronesa cattle is a traditional Portuguese breed used for meat production commercialized with PDO—Protected Designation of Origin [ 31 , 32 ]. Maronesa cattle is considered a threatened breed that has been used for centuries in agricultural work [ 32 ]. Therefore, in this study, we isolated S. aureus from farm workers, cows, and their environments and aimed to find evidence of bacterial transmission and spread investigating the antimicrobial resistance and genetic lineages of the isolates.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective

et al. 2022

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Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.

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“…A standardized suspension (0.5 McFarland) of S. aureus isolates were prepared and inoculated onto ORSAB medium pre-supplemented with 6 μg/ml oxacillin and 4% sodium chloride and incubated at 35 0 C overnight. The emergent bluish colonies from the overnight cultures were considered methicillin resistant (25).…”

Section: Oxacillin Resistance Screening Agar Base (Orsab) Testmentioning

confidence: 99%

Constellation of methicillin-resistant genomic islands (SCCmec) among nasal meticillin-resistant Staphylococcus aureus isolates

Suleiman¹,

Ademola²,

Olalekan³

2021

Acta fac medic Naissensis

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The apprehensiveness for the knowledge vacuum on existential threat of nasal carriage of pvl+ healthcare-acquired meticillin-resistant Staphylococcus aureus (HA-MRSA) strains amongst subjects in hospitals have led us to pursue a grasp on the constellation of staphylococcal cassette chromosome mec (SCCmec) types and pvl gene among mecA positive MRSA nasal strains. This was accomplished by phenotypic (catalase, coagulase, Microgen staph ID, ORSAB) and genotypic (polymerase chain reaction) biotyping techniques. All the mecA+ strains harboured the SCCmec gene; SCCmec type I prevailed in 43.75% and pvl was found in 42.1% of the isolates. Dual carriage of mecA and pvl genes occurred in six (37.5%, n = 6/16) strains. Overall, majority of the mecA+ MRSA strains documented in this study carried SCCmec elements of the HA genotype with a hint of community-acquired (CA)genotype suggesting a possible coexistence of both HA-MRSA and community-acquiredhealthcare-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) strains. Consequently, the implementation of methodical surveillance is needed for the evaluation of potential shifts in directionality of (HA-MRSA/CA-MRSA) pvl+ MRSA clones in our hospitals for effective and prudent antimicrobial stewardship.

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One Health Approach Reveals the Absence of Methicillin-Resistant Staphylococcus aureus in Autochthonous Cattle and Their Environments

Cited by 14 publications

References 18 publications

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective

Constellation of methicillin-resistant genomic islands (SCCmec) among nasal meticillin-resistant Staphylococcus aureus isolates

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