One‐pot green synthesis and structural characterisation of silver nanoparticles using aqueous leaves extract of Carissa carandas : antioxidant, anticancer and antibacterial activities

Abstract

Facile green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of () leaves was studied. Fabrication of AgNPs was confirmed by the UV-visible spectroscopy which gives absorption maxima at 420 nm. C. carandas leaves are the rich source of the bioactive molecules, acts as a reducing and stabilising agent in AgNPs, confirmed by Fourier transforms infrared spectroscopy. The field emission scanning electron microscope revealed the spherical shape of biosynthesised AgNPs. A distinctive peak of silver at 3 keV was determined by energy dispersive X-ray spectroscopy. X-ray diffraction showed the facecentred cubic structure of biosynthesised AgNPs and thermal stability was confirmed by the thermogravimetric analysis. Total flavonoid and total phenolic contents were evaluated in biosynthesised AgNPs. Biosynthesised AgNPs showed free radical scavenging activities against 2, 2-diphenyl-1-picrylhydrazyl test and ferric reducing antioxidant power assay. cytotoxicity against hepatic cell lines (HUH-7) and renal cell lines (HEK-293) were also assessed. Finally, biosynthesised AgNPs were scrutinised for their antibacterial activity against methicillin-resistant, , and . This study demonstrated the biofabrication of AgNPs by using leaves extract and a potential in vitro biological application as antioxidant, anticancer and antibacterial agents.

Environmentally friendly green approach for the fabrication of silver oxide nanoparticles: Characterization and diverse biomedical applications

“…The mortality at different doses and IC 50 value are illustrated in Figure 6a. The results of our study are consistent with the previous studies of green AgO 2 NPs from Talaromyces purpurogenus and Dolichos lablab using HepG2 cell line and Carissa carandas using HUH‐7 cancer cells (Bhatnagar, Kobori, Ganesh, Ogawa, & Aoyagi, 2019; Kahsay et al, 2018; Singh et al, 2018).…”

“…The HepG2 and HUH-7 cells were treated with various concentrations of AgO 2 NPs ranging from 7.31 to 900 μg/ml for 24 h. Dose-dependent inhibition potentials were observed against HepG2 and HUH-7 cells. The results of our study are consistent with the previous studies of green AgO 2 NPs from Talaromyces purpurogenus and Dolichos lablab using HepG2 cell line and Carissa carandas using HUH-7 cancer cells (Bhatnagar, Kobori, Ganesh, Ogawa, & Aoyagi, 2019;Kahsay et al, 2018;Singh et al, 2018).…”

The potential antimalarial efficacy of hemocompatible silver nanoparticles from Artemisia species against P. falciparum parasite

“…Thus, the preparation of eco-friendly metal nanoparticles using biological fluids represents a field of technology for nanomedicine applications which is growing with impressive speed [22]. Generally, extracts from plants, bacteria and algae, plant-based phytochemicals, and other biological sources are used as the reducing agents in the synthesis of metal nanoparticles with high stability and low toxicity [23][24][25][26][27].…”

Prospective Application of Nanoparticles Green Synthesized Using Medicinal Plant Extracts as Novel Nanomedicines

“… Antitussive, antiulcer, antioxidant, antibacterial and anticancer ZnO Anticancer and antibacterial [ 97 ] Calotropis gigantea (L.) Dryand. Purgative, anthelmintic, anticonvulsant, sedative and antipyretic CdS Antimicrobial [ 98 ] MgO None tested [ 70 ] Carissa carandas L. Used in the treatment of scabies, intestinal worms, pruritus, antiscorbutic, anthelmintic, pain relieving, cancer and hepatoprotective Ag Antioxidant, anticancer and antibacterial [ 99 ] Catharanthus roseus (L.) G. Don Anticancer, astringent, antibacterial, antidiabetic, antifungal, and antimalarial Ag, ZnO Antimicrobial [ 100 , 101 ] Catunaregam spinosa (Thunb.) Tirveng.…”