One-pot synthesis of a DNA-anchored SERS nanoprobe with simultaneous nanostructural tuning and Raman reporter encoding

Abstract

We report a new simple one-pot synthesis method for a DNA-anchored SERS nanoprobe encoded with Raman reporters.

Gold Nanobones Enhanced Ultrasensitive Surface-Enhanced Raman Scattering Aptasensor for Detecting Escherichia coli O157:H7

“…Not only were more Raman dyes embedded, but the GNR Apt-1+RhB generated more local electromagnetic field "hotpots". 44 Comediation of the GNRs by Apt-1 and RhB formed GNR Apt-1+RhB with a NB structure, which provided a "lightning-rod" effect at each end of the NB. Meanwhile, simulation diagrams and electromagnetic field strength distributions of the original GNRs and different assembly modes (i.e., separated, end-to-end, side-byside, and end-to-side) of the GNR Apt-1+RhB were generated using the Maya and FDTD programs, respectively (Figure 2F).…”

“…The dominant SERS enhancement was attributed to the enhanced electromagnetic behavior of the geometrically defined SPRs of the metal nanostructures. Not only were more Raman dyes embedded, but the GNR Apt‑1+RhB generated more local electromagnetic field “hotpots” . Comediation of the GNRs by Apt-1 and RhB formed GNR Apt‑1+RhB with a NB structure, which provided a “lightning-rod” effect at each end of the NB.…”

Printed Nanochain‐Based Colorimetric Assay for Quantitative Virus Detection

“…In contrast, the SERS offers higher detection sensitivity that enables optical tracking of individual biological particles [14] . However, they require precise adjustment of the incident angle of probing radiation [15] . Whispering gallery resonators also are capable of remarkably improving the detection limit on basis of the strong resonance of nanostructures [16] .…”

Printed Nanochain‐Based Colorimetric Assay for Quantitative Virus Detection

“…[14] However,t hey require precise adjustment of the incident angle of probing radiation. [15] Whispering gallery resonators also are capable of remarkably improving the detection limit on basis of the strong resonance of nanostructures. [16] It allows for detecting and analyzing single viral particles,but lacks the ability to sense the load of analytes in aconcentrated solution.…”